陈瑶,王树雪,魏艳敏,赵晓燕,尚巧霞,刘正坪

• 1:农业应用新技术北京市重点实验室·植物科学技术学院北京农学院

摘要(Abstract)：

【目的】针对近年来设施草莓根腐病普遍发生并逐年加重的现状,明确北京地区引起设施草莓根腐病的主要致病真菌的种类。【方法】以草莓根腐病(Strawberry root rot)为研究对象,采用组织分离法,从病株上共分离纯化得到5个菌株,对主要致病菌C16-4进行了鉴定和生物学特性研究。【结果】结果表明,最利于C16-4生长和产孢的培养基为PDA;最佳氮源为蛋白胨,最佳碳源为麦芽糖和淀粉。菌丝在10~35℃均可以生长,在本试验所设温度范围内,最适生长和产孢的温度分别为25℃和30℃。菌丝在pH值为5~7时均可以生长,最适pH值为7,中性条件下利于产孢。不同光照条件对菌丝生长影响不明显,在全黑暗情况下产孢量最大。【结论】通过培养性状、形态特征、致病性测定结果和ITS序列分析,将C16-4鉴定为尖孢镰刀菌(Fusarium oxysporium Schlecht)。

关键词(KeyWords)： 草莓根腐病菌;鉴定;致病性测定;分子鉴定;生物学特性

Abstract:

Keywords:

基金项目(Foundation): 北京市属高等学校人才强教计划(编号:PHR201107135);; 北京市科技新星计划(编号:2007-B-032)

作者(Author): 陈瑶,王树雪,魏艳敏,赵晓燕,尚巧霞,刘正坪

DOI: 10.13925/j.cnki.gsxb.2012.04.005

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