戚霄晨;简在海;张琦;宋尚伟;

• 1:河南农业大学园艺学院·河南省果树瓜类生物学重点实验室

摘要(Abstract)：

【目的】探讨叶面施用外源硒对甜樱桃叶片、果实中的硒及有害重金属含量、部分生化指标、果实品质的影响,以期为富硒甜樱桃的生产提供理论依据。【方法】以‘美早’甜樱桃为试材,选取亚硒酸钠(Na2SeO3)溶液为硒源,设置0、5、10、20和40 mg·L-1等5个质量浓度水平,于落花后喷施叶片,1周后再喷施1次。【结果】甜樱桃果实和叶片中的硒含量随施硒浓度的增加而递增,重金属镉和铅的含量均有不同程度的降低。叶面喷硒可显著提高甜樱桃叶片和果实中超氧化物岐化酶(SOD)的酶活性,降低丙二醛(MDA)的活性,减少脯氨酸(Pro)的积累。果实维生素C含量增加、可滴定酸含量降低、果实品质改善。【结论】叶面施硒可以提高甜樱桃果实品质,降低部分重金属的含量。

关键词(KeyWords)： 甜樱桃;硒;叶面喷施;果实品质;重金属

Abstract:

Keywords:

基金项目(Foundation): 河南省科技厅基础与前沿项目“甜樱桃内参基因的筛选与MADS-Box基因的克隆及表达分析”(152300410074);; 河南省果树瓜类生物学重点实验室研究基金

作者(Author): 戚霄晨;简在海;张琦;宋尚伟;

Email:

参考文献(References)：

• [1]焦中高,刘杰超,王思新.甜樱桃采后生理与贮藏保鲜[J].果树学报,2003,20(6):498-502.JIAO Zhonggao,LIU Jiechao,WANG Sixin.Postharvest physiology and storage of sweet cherry[J].Journal of Fruit Science,2003,20(6):498-502.
• [2]郑海涛,邓正春,杨宇,彭永胜,彭杰,孙元学.富硒樱桃优质高产栽培技术[J].湖南农业科学,2011(12):38-39.ZHENG Haitao,DENG Zhengchun,YANG Yu,PENG Yongsheng,PENG Jie,SUN Yuanxue.High-quality and high-yield cultivation techniques of selenium-enriched cherry[J].Hunan Agricultural Sciences,2011(12):38-39.
• [3]倪静安.微量元素硒、自由基与健康的关系[J].无锡轻工业学院学报,1992,11(3):27.NI Jing’an.Relationship between trace elements selenium,free radicals and health[J].Journal of Wuxi Institute of Light Industry,1992,11(3):27.
• [4]史丽英,罗世炜.微量元素硒的研究进展[J].襄樊职业技术学院学报,2005,4(1):108-110.SHI Liying,LUO Shiwei.Research advance of trace element selenium[J].Journal of Xiangfan Vocational and Technical College,2005,4(1):108-110.
• [5]彭耀湘,陈正法.硒的生理功能及富硒水果的开发利用[J].农业现代化研究,2007,28(3):381-384.PENG Yaoxiang,CHEN Zhengfa.Physiological function of selenium and development and utilization of selenium-rich fruits[J].Agricultural Modernization Research,2007,28(3):381-384.
• [6]BROADLEY M R,WHITE P J,BRYSON R J,MEACHAM MC,BOWEN H C,JOHNSON S E,HAWKESFORD M J,MC-GRATH S P,ZHAO F J,BREWARD NET A L.Biofortification of UK food crops with selenium[J].Proceeding of the Nutrition Society,2006,65(2):169-181.
• [7]TSAI C F,OU B R,LIANG Y C,YEH J Y.Growth inhibition and antioxidative status induced by selenium-enriched broccoli extract and selenocompounds in DNA mismatch repair-deficient human colon cancer cells[J].Food Chemisty,2013,139(1-4):267-273.
• [8]邓小芳,吕臣浩,黄立强,张海清,赵竹青,刘新伟.喷施时期和硒源对‘金桃’猕猴桃硒吸收累积及主要品质指标的影响[J].果树学报,2018,35(11):1385-1392.DENG Xiaofang,LüChenhao,HUANG Liqiang,ZHANG Haiqing,ZHAO Zhuqing,LIU Xinwei.Effects of spraying time and selenium source on selenium absorption and accumulation and main quality indexes of Kiwifruit‘Jintao’[J].Journal of Fruit Science,2018,35(11):1385-1392.
• [9]余俊,赵玉华,白描,杨国顺.钙处理对葡萄果实花青素含量及品质的影响[J].果树学报,2018,35(6):741-746.YU Jun,ZHAO Yuhua,BAI Miao,YANG Guoshun.Effects of calcium treatment on anthocyanin content and quality of grape fruits[J].Journal of Fruit Science,2018,35(6):741-746.
• [10]赵春梅,曹启民,唐群锋,李晓波.植物富硒规律的研究进展[J].热带农业科学,2010,30(7):82-86.ZHAO Chunmei,CAO Qimin,TANG Qunfeng,LI Xiaobo.Research advances on selenium enrichment laws of plants[J].Tropical Agricultural Sciences,2010,30(7):82-86.
• [11]周勋波,吴海燕,洪延生,谢甫绨.作物施硒研究进展[J].中国农业科技导报,2002,4(6):45-48.ZHOU Xunbo,WU Haiyan,HONG Yansheng,XIE Futi.Research progress in crop selenium application[J].China Agricultural Science and Technology Guide,2002,4(6):45-48.
• [12]王庆华,黄伟,李前勇,贺永建,张德志,胥清芳.中国富硒食品的生产现状及趋势[J].广东微量元素科学,2008,15(3):7-10.WANG Qinghua,HUANG Wei,LI Qianyong,HE Yongjian,ZHANG Dezhi,XU Qingfang.Production status and trend of selenium-enriched food in China[J].Guangdong Microelement Science,2008,15(3):7-10.
• [13]吴永尧,彭振坤,罗泽民.硒的多重生物学功能及对人和动物健康的影响[J].湖南农业大学学报,1997,23(3):294-299.WU Yongyao,PENG Zhenkun,LUO Zemin.Multiple biological functions of selenium and its effects on human and animal health[J].Journal of Hunan Agricultural University,1997,23(3):294-299.
• [14]王权,李秀霞,李伦,田金徽,杨克虎,吴泰相,廖永健.硒与大骨节病相关性的Meta分析[J].中国循证医学杂志,2013,13(12):1421-1430.WANG Quan,LI Xiuxia,LI Lun,TIAN Jinhui,YANG Kehu,WU Taixiang,LIAO Yongjian.Meta-analysis of correlation between selenium and kashin-deck disease[J].Chinese Journal of Evidence-Based Medicine,2013,13(12):1421-1430.
• [15]李丽辉,林亲录.我国富硒食品的研究进展[J].中国食物与营养,2007(2):23-25.LI Lihui,LIN Qinlu.Research progress of selenium-enriched food in China[J].Food and Nutrition in China,2007(2):23-25.
• [16]王晋民,赵之重,段冰.叶面施硒对不同蔬菜硒富集和产量的影响[J].西北农林科技大学学报,2007,35(7):108-114.WANG Jinmin,ZHAO Zhizhong,DUAN Bing.Effects of foliar selenium application on selenium enrichment and yield of different vegetables[J].Journal of Northwest Agriculture and Forestry University,2007,35(7):108-114.
• [17]宁婵娟,丁宁,吴国良,冀爱青,刘晓华,杨燕君.喷硒时期与浓度对红富士苹果果实品质及各部位全硒和有机态硒含量的影响[J].植物营养与肥料学报,2013,19(5):1109-1117.NING Chanjuan,DING Ning,WU Guoliang,JI Aiqing,LIUXiaohua,YANG Yanjun.Effects of selenium spraying time and concentration on fruit quality and content of total selenium and organic selenium in various parts of Fuji apple[J].Journal of Plant Nutrition and Fertilizer,2013,19(5):1109-1117.
• [18]罗盛国,徐宁彤,刘元英.叶面喷硒提高粮食中的硒含量[J].东北农业大学学报,1999,30(1):18-22.LUO Shengguo,XU Ningtong,LIU Yuanying.Increase of selenium content in grain with foliar spraying selenium[J].Journal of Northeast Agricultural University,1999,30(1):18-22.
• [19]张东威.中国土壤中硒及其土壤环境质量标准研究[J].水土保持研究,1994(增刊):112.ZHANG Dongwei.Study on selenium in Chinese soil and its soil environmental quality standards[J].Soil and Water Conservation Research,1994(Suppl.):112.
• [20]孙瑞莲,周启星.高等植物重金属耐性与超积累特性及其分子机理研究[J].植物生态学报,2005,29(3):497-504.SUN Ruilian,ZHOU Qixing.Research on heavy metal tolerance and hyperaccumulation characteristics in higher plants and their molecular mechanism[J].Journal of Plant Ecology,2005,29(3):497-504.
• [21]方勇,陈曦,陈悦,罗佩竹,杨文建,马宁,辛志宏,赵立艳,胡秋辉.外源硒对水稻籽粒营养品质和重金属含量的影响[J].江苏农业学报,2013,29(4):760-765.FANG Yong,CHEN Xi,CHEN Yue,LUO Peizhu,YANG Wenjian,MA Ning,XIN Zhihong,ZHAO Liyan,HU Qiuhui.Effects of exogenous selenium on nutritional quality and heavy metal content of rice grain[J].Jiangsu Journal of Agricultural Sciences,2013,29(4):760-765.
• [22]张海英,韩涛,田磊,王有年.草莓叶面施硒对其重金属镉和铅积累的影响[J].园艺学报,2011,38(3):409-416.ZHANG Haiying,HAN Tao,TIAN Lei,WANG Younian.Effects of selenium application on cadmium and lead accumulation in strawberry leaves[J].Acta Horticultuare Sinica,2011,38(3):409-416.
• [23]荀黎红,吴丛雅.高硒与疾病关系的研究进展[J].国外医(学医学地理分册),2005,26(1):106-108.XUN Lihong,WU Congya.Research advances on high selenium and diseases[J].Foreign Medical Sciences(Section of Medgeography),2005,26(1):106-108.
• [24]窦俊辉,喻树迅,范术丽,庞朝友,宋美珍.SOD与植物胁迫抗性[J].分子植物育种,2010,8(2):359-364.DOU Junhui,YU Shuxun,FAN Shuli,PANG Chaoyou,SONGMeizhen.SOD and plant stress resistance[J].Molecular plan Breeding,2010,8(2):359-364.
• [25]李彦,史衍玺,张英鹏,董晓霞,孙明.盐胁迫条件下硒对小白菜抗氧化活性及膜脂过氧化作用的影响[J].植物营养与肥料学报,2008,14(4):749-753.LI Yan,SHI Yanxi,ZHANG Yingpeng,DONG Xiaoxia,SUNMing.Effects of selenium on antioxidant activity and membrane lipid peroxidation of pakchoi under salt stress[J].Journal of Plant Nutrition and Fertilizer,2008,14(4):749-753.
• [26]周大寨,唐巧玉,张驰,吴永尧.硒对花椰菜幼苗叶绿素、脯氨酸及丙二醛含量的影响[J].湖北农业科学,2005(2):28.ZHOU Dazhai,TANG Qiaoyu,ZHANG Chi,WU Yongyao.Effects of selenium on chlorophyll,proline and malondialdehyde contents in cauliflower seedlings[J].Hubei Agricultural Sciences,2005(2):28.
• [27]张弛,吴永尧,彭振坤,周大寨.硒对油菜苗期脯氨酸和丙二醛含量的影响[J].湖北农业科学,2003(2):16.ZHANG Chi,WU Yongyao,PENG Zhenkun,ZHOU Dazhai.Effects of selenium on proline and malondialdehyde content in rape seedling[J].Hubei Agricultural Sciences,2003(2):16.
• [28]刘仁道,黄仁华,吴世权,何仕松.外源Na2SeO3对红阳猕猴桃果实品质、不同组织硒含量及形态的影响[J].食品工业科技,2012,15(33):23-35.LIU Rendao,HUANG Renhua,WU Shiquan,HE Shisong.Effects of exogenous Na2SeO3on fruit quality,selenium content and morphology in different tissues of actinidia chinensis[J].Food Industry Science and Technology,2012,15(33):23-35.