王立新;梁文杰;谭晓风;李秀根;张琳;

• 1:温州科技职业学院
• 2:中南林业科技大学经济林育种与栽培国家林业局重点实验室
• 3:中国农业科学院郑州果树研究所

摘要(Abstract)：

梨是典型的配子体自交不亲和果树,生产上需要配置授粉树才能获得理想的产量。鉴定梨品种的S基因型能够为合理配置授粉树提供理论依据。为鉴定S基因型,设计特异性引物对6个中国原产梨品种的基因组DNA进行扩增,片段回收、克隆、测序。使用生物信息学软件对各序列进行分析,确定6个梨品种S基因型分别为:宝珠S22S42,半斤酥S5S21,张掖长把S19S22,贵德长把S19S22,满顶雪S4S15,水冬瓜S15S45。其中S45为新分离鉴定的梨S-RNase基因,GenBank登录号为:EF643634。S45和梨S26的相似性最高,但高变区有5个氨基酸差异。

关键词(KeyWords)： 梨;自交不亲和性;S基因型;鉴定

Abstract:

Keywords:

基金项目(Foundation): 国家林业局重点科学技术研究计划林业新技术开发与储备专项(2006-12);国家林业局重点项目;; 湖南省科技计划(编号:00JZYZ115);;

作者(Author): 王立新;梁文杰;谭晓风;李秀根;张琳;

Email:

DOI: 10.13925/j.cnki.gsxb.2010.05.005

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