李秋利;高登涛;魏志峰;王志强;刘军伟;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】探究桃叶片在不同SO_2浓度梯度胁迫下的叶绿素荧光参数及抗氧化酶活性的变化。【方法】以1 a(年)生桃盆栽嫁接苗为试材,测定分析不同SO_2浓度梯度胁迫下叶片叶绿素荧光动力学参数、抗氧化酶活性、丙二醛(MDA)含量的变化。【结果】(1) Fm、Fv/Fm、Fv/F0均随SO_2浓度的升高而降低。随处理时间的延长,0.14 mg·m~(-3)处理和对照相比差异不显著,1.43 mg·m~(-3)和14.3 mg·m~(-3)处理显著下降。(2)随着SO_2浓度升高和处理时间的延长,qP、Y(Ⅱ)逐渐下降,而qN、Y(NPQ)逐渐上升,Y(NO)稳定在较低水平。(3)在O-J-I-P曲线中,中、高浓度SO_2处理使JIP点荧光强度出现不同程度的降低,并降低了FPo、FEo、ψo和光合机构性能指数(PIABS、PICSo和PICSm);不同SO_2浓度处理对F0、Fp、VJ、Mo的影响不显著。(4) 0.14 mg·m~(-3)SO_2浓度处理的叶片抗氧化酶活性和MDA含量与对照相比差异不显著,1.43 mg·m~(-3)和14.3 mg·m~(-3)SO_2浓度处理的叶片抗氧化酶活性和MDA含量高于对照,14.3 mg·m~(-3)处理的SOD、POD和CAT活性先升高后降低,MDA含量则逐渐升高。(5)用荧光诱导动力学曲线可以快速检测桃叶片在二氧化硫胁迫下的生理响应。【结论】0.14 mg·m~(-3)SO_2处理对叶片的光合机构无显著影响,1.43 mg·m~(-3)和14.3 mg·m~(-3)SO_2胁迫下,叶片PSⅡ反应中心关闭或不可逆失活,表现出光抑制,14.3 mg·m~(-3)SO_2胁迫影响更显著。

关键词(KeyWords)： 桃;SO2胁迫;叶绿素荧光特性;抗氧化酶活性

Abstract:

Keywords:

基金项目(Foundation): 中国农业科学院基本科研业务费专项(1613112017205);中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2018-ZFRI)

作者(Author): 李秋利;高登涛;魏志峰;王志强;刘军伟;

Email:

DOI: 10.13925/j.cnki.gsxb.20170501

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