李秋利;高登涛;魏志峰;王志强;刘军伟;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】探究桃叶片在不同SO_2浓度梯度胁迫下的叶绿素荧光参数及抗氧化酶活性的变化。【方法】以1 a(年)生桃盆栽嫁接苗为试材,测定分析不同SO_2浓度梯度胁迫下叶片叶绿素荧光动力学参数、抗氧化酶活性、丙二醛(MDA)含量的变化。【结果】(1) Fm、Fv/Fm、Fv/F0均随SO_2浓度的升高而降低。随处理时间的延长,0.14 mg·m~(-3)处理和对照相比差异不显著,1.43 mg·m~(-3)和14.3 mg·m~(-3)处理显著下降。(2)随着SO_2浓度升高和处理时间的延长,qP、Y(Ⅱ)逐渐下降,而qN、Y(NPQ)逐渐上升,Y(NO)稳定在较低水平。(3)在O-J-I-P曲线中,中、高浓度SO_2处理使JIP点荧光强度出现不同程度的降低,并降低了FPo、FEo、ψo和光合机构性能指数(PIABS、PICSo和PICSm);不同SO_2浓度处理对F0、Fp、VJ、Mo的影响不显著。(4) 0.14 mg·m~(-3)SO_2浓度处理的叶片抗氧化酶活性和MDA含量与对照相比差异不显著,1.43 mg·m~(-3)和14.3 mg·m~(-3)SO_2浓度处理的叶片抗氧化酶活性和MDA含量高于对照,14.3 mg·m~(-3)处理的SOD、POD和CAT活性先升高后降低,MDA含量则逐渐升高。(5)用荧光诱导动力学曲线可以快速检测桃叶片在二氧化硫胁迫下的生理响应。【结论】0.14 mg·m~(-3)SO_2处理对叶片的光合机构无显著影响,1.43 mg·m~(-3)和14.3 mg·m~(-3)SO_2胁迫下,叶片PSⅡ反应中心关闭或不可逆失活,表现出光抑制,14.3 mg·m~(-3)SO_2胁迫影响更显著。

关键词(KeyWords)： 桃;SO2胁迫;叶绿素荧光特性;抗氧化酶活性

Abstract:

Keywords:

基金项目(Foundation): 中国农业科学院基本科研业务费专项(1613112017205);中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2018-ZFRI)

作者(Author): 李秋利;高登涛;魏志峰;王志强;刘军伟;

Email:

DOI:

参考文献(References)：

• [1]高登涛,李秋利,魏志峰,王志强,贺保国,司鹏.植物对二氧化硫胁迫反应与应答机制研究进展[J].广东农业科学,2016,43(11):27-35.GAO Dengtao,LI Qiuli,WEI Zhifeng,WANG Zhiqiang,HE Baoguo,SI Peng. Research progress on damage reaction and response mechanism of plants to sulfur dioxide stress[J]. Guangdong Agricultural Sciences,2016,43(11):27-35.
• [2]李利红,仪慧兰,王磊,李秀芬.二氧化硫暴露对拟南芥叶片形态和生理生化指标的影响[J].农业环境科学学报,2008,27(2):525-529.LI Lihong,YI Huilan,WANG Lei,LI Xiufen. Effects of sulfur dioxide on the morphological and physiological biochemical parameters in Arabidopsis thaliana plants[J]. Journal of Agro-Environment Science,2008,27(2):525-529.
• [3]李向应.SO2对四种李属彩叶树木生理特性及叶片色素的影响研究[D].呼和浩特:内蒙古农业大学,2007.LI Xiangying. Effect of sulfur dioxide stress on physiological characteristics and pigment of four species of colored-leaf trees of prunus[D]. Hohhot:Inner Mongolia Agricultural University,2007.
• [4]刘清丽.植物对SO2的伤害反应及生化抗性研究[D].太谷:山西农业大学,2003.LIU Qingli. Study on injury response and biochemical resistantance to SO2of plants[D]. Taigu:Shanxi Agricultural University,2003.
• [5] LIU N,PENG C L,LIN Z F,LIN G Z,ZHANG L L,PAN X P.Changes in photosystemⅡactivity and leaf reflectance features of several subtropical woody plants under simulated SO2treatment[J]. Journal of Integrative Plant Biology,2006,48(11):1274-1286.
• [6]李钦夫,李征明,纪建伟,邹秋滢,于辉.叶绿素荧光动力学及在植物抗逆生理研究中的应用[J].湖北农业科学,2013,52(22):5399-5402.LI Qinfu,LI Zhengming,JI Jianwei,ZOU Qiuying,YU Hui. Applications of chlorophyll fluorescence kinetics in the physiological resistance studies of plant[J]. Hubei Agricultural Sciences,2013,52(22):5399-5402.
• [7] KAUTSKY H,HIRSCH A. Neue versuche zur kohlens?ureassimilation[J]. Naturwissenschaften,1931,19(48):964.
• [8]钱永强,周晓星,韩蕾,孙振元,巨关升. Cd2+胁迫对银芽柳PSⅡ叶绿素荧光光响应曲线的影响[J].生态学报,2011,31(20):6134-6142.QIAN Yongqiang,ZHOU Xiaoxing,HAN Lei,SUN Zhenyuan,JU Guansheng. Rapid light-response curves of PSⅡchlorophyll fluorescence parameters in leaves of Salixleucopithecia subjected to cadmium-ion stress[J]. Acta Ecologica Sinica,2011,31(20):6134-6142.
• [9]赵丽英,邓西平,山仑.渗透胁迫对小麦幼苗叶绿素荧光参数的影响[J].应用生态学报,2005,16(7):1261-1264.ZHAO Liying,DENG Xiping,SHAN Lun. Effects of osmotic stress on chlorophyll fluorescence parameters of wheat seedling[J]. Chinese Journal of Applied Ecology,2005,16(7):1261-1264.
• [10] GUIDI L,LANDI M,PENELLA C,CALATAYUD A. Application of modulated chlorophyll fluorescence and modulated chlorophyll fluorescence imaging in studying environmental stresses effect[J]. Annali di Botanica,2016,6:5-22.
• [11]全绍文,曹福军,牛建新.新疆早实核桃和晚实核桃叶绿素荧光特征差异比较[J].果树学报,2016,33(7):832-840.QUAN Shaowen,CAO Fujun,NIU Jianxin. A comparative study of chlorophyll fluorescence characteristics between early fruiting and late fruiting walnut cultivars in Xinjiang[J]. Journal of Fruit Science,2016,33(7):832-840.
• [12]马宏,孟红志,李世军,李佳,李忠勇,张学英,徐继忠.不同光质对‘春美’桃光合特性和果实品质的影响[J].果树学报,2017,34(7):835-842.MA Hong,MENG Hongzhi,LI Shijun,LI Jia,LI Zhongyong,ZHANG Xueying,XU Jizhong. Effects of light quality on photosynthetic characteristics and fruit quality of‘Chunmei’peach[J]. Journal of Fruit Science,2017,34(7):835-842.
• [13]牛良,王志强,刘淑娥,宋银花,鲁振华,宗学普.早熟桃新品种‘春蜜’[J].园艺学报,2010,37(12):2029-2030.NIU Liang,WANG Zhiqiang,LIU Shu’e,SONG Yinhua,LU Zhenhua,ZONG Xuepu. A new early maturing peach cultivar‘Chunmi’[J]. Acta Horticulturae Sinica,2010,37(12):2029-2030.
• [14]刘子琪. SO2胁迫下园林植物叶片含硫量及光谱特征研究[D].武汉:华中农业大学,2014.LIU Ziqi. Research on sulfur content and spectral characteristics of garden leaves under SO2stress[D]. Wuhan:Huazhong Agricultural University,2014.
• [15] GENTY B,BRIANTAIS J M,BAKER N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J]. Biochimica et Biophysica Acta,1989,990(1):87-92.
• [16] STIRBET A,GOVINDJEE. On the relation between the Kautsky effect(chlorophyll a fluorescence induction)and photosystemⅡ:basics and applications of the OJIP fluorescence transient[J]. Journal of Photochemistry and Photobiology B:Biology,2011,104(1):236-257.
• [17] CEPPI M G,OUKARROUM A,CICEK N,STRASSER R J,SCHANSKER G. The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves:a study on plants exposed to magnesium and sulfate deficiencies,drought stress and salt stress[J]. Physiologia Plantarum,2012,144(3):277-288.
• [18] DHINDSA R S,PLUMB-DHINDSA P,THORPE T A. Leaf senescence:correlated with increased levels of membrane permeability and lipid peroxidation,and decreased levels of superoxide dismutase and catalase[J]. Journal of Experimental Botany,1981,32(1):93-101.
• [19] CHANCE B,MAEHLY A C. Assay of catalases and peroxidases[J]. Methods in Enzymology,1955,2:764-775.
• [20] AEBI H. Catalase in vitro[J]. Methods in Enzymology,1984,105:121-126.
• [21] ZHANG J,KIRKHAM M B. Drought-stress-induced changes in activities of superoxide dismutase,catalase,and peroxidase in wheat species[J]. Plant and Cell Physiology,1994,35(5):785-791.
• [22]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.ZHANG Shouren. A discussion on chlorophyll fluorescence kinetics parameters and their significance[J]. Chinese Bulletin of Botany,1999,16(4):444-448.
• [23]吴甘霖,段仁燕,王志高,张中信,吴礼凤.干旱和复水对草毒叶片叶绿素荧光特性的影响[J].生态学报,2010,30(14):3941-3946.WU Ganlin,DUAN Renyan,WANG Zhigao,ZHANG Zhongxin,WU Lifeng. Effects of drought stress and rehydration on chlorophyll fluorescence characteristics in Fragaria×ananassa Duch.[J]. Acta Ecologica Sinica,2010,30(14):3941-3946.
• [24]耿东梅,单立山,李毅,ЖигуновАнатолийВасильевич.土壤水分胁迫对红砂幼苗叶绿素荧光和抗氧化酶活性的影响[J].植物学报,2014,49(3):282-291.GENG Dongmei,SHAN Lishan,LI Yi,ЖигуновАнатолийВасильевич. Effect of soil water stress on chlorophyll fluorescence and antioxidant enzyme activity in Reaumuria soongorica seedlings[J]. Chinese Bulletin of Botany,2014,49(3):282-291.
• [25]李彦慧.李属彩叶树种耐SO2机制研究[D].保定:河北农业大学,2008.LI Yanhui. Study on mechanism of SO2tolerance of colores-leaf tree species in Prunus[D]. Baoding:Agricultural University of Hebei,2008.
• [26]周蕴薇,刘艳萍,戴思兰.用叶绿素荧光分析技术鉴定植物抗寒性的剖析[J].植物生理学通讯,2006,42(5):945-950.ZHOU Yunwei,LIU Yanping,DAI Silan. Identification of cold resistant plants by chlorophyll fluorescence analysis technique[J]. Plant Physiology Communications,2006,42(5):945-950.
• [27]张国良,陈文军. 1,2,4-三氯苯胁迫对水稻苗期叶绿素荧光特性的影响[J].植物生理学报,2016,52(5):628-634.ZHANG Guoliang,CHEN Wenjun. Effects of 1,2,4-trichlorobenzene on chlorophyll fluorescence characteristics of rice seedlings[J]. Plant Physiology Journal,2016,52(5):628-634.
• [28] KRAUSE G H,WEIS E. Chlorophyll fluorescence and photosynthesis:the basics[J]. Annual Review of Plant Biology,1991,42(1):313-349.
• [29]侯利钦. SO2胁迫对红花檵木生理特性的影响[D].成都:四川农业大学,2015.HOU Liqin. The effects of SO2stress on physiological characteristics of Loropetalum chinense var. rubrum[D]. Chengdu:Sichuan Agricultural University,2015.
• [30] KRAMER D M,JOHNSON G,KIIRATS O,EDWARDS G E.New fluorescence parameters for the determination of QA redox state and excitation energy fluxes[J]. Photosynthesis Research,2004,79(2):209-218.
• [31]李鹏民.快速叶绿素荧光诱导动力学在植物逆境生理研究中的应用[D].泰安:山东农业大学,2007.LI Pengmin. Application of chlorophyll a fluorescence transient in study of plant physiology under stress conditions[D]. Taian:Shandong Agricultural University,2007.
• [32] STRASSERF R J,SRIVASTAVA A. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria[J]. Photochemistry and Photobiology,1995,61(1):32-42.
• [33] STRASSER R J,SRIVASTAVA A,TSIMILLI-MICHAEL M.The fluorescence transient as a tool to characterize and screen photosynthetic samples[M]//YUNUS M,PATHRE U,MOHANTY P. Probing photosynthesis:mechanisms,regulation and adaptation. Taylor and Francis,London,Probing Photosynthesis:mechanisms,regulation and adaptation,2000:445-483.
• [34] STRASSER R J,TSIMILLI-MICHAEL M,SRIVASTAVA A.Analysis of the chlorophyll a fluorescence transient[J]. PAPAGEORGIOU G,GOVINDJEE. Advances in Photosynthesis and Respiration. Netherlands:KAP Press,2004:1-42.
• [35]李鹏民,高辉远.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.LI Pengmin,GAO Huiyuan. Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study[J]. Journal of Plant Physiology and Molecular Biology,2005,31(6):559-566.
• [36] APPENROTH K J,ST?CKEL J,SRIVASTAVA A,STRASSER R J. Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements[J]. Environmental Pollution,2001,115(1):49-64.
• [37] VAN HEERDEN P D R,STRASSER R J,KRüGER G H J. Reduction of dark chilling stress in N2-fixing soybean by nitrate as indicated by chlorophyll a fluorescence kinetics[J]. Physiologia Plantarum,2004,121(2):239-249.
• [38] VAN HEERDEN P D R,TSIMILLI-MICHAEL M,KRüGER G H J,STRASSER R J. Dark chilling effects on soybean genotypes during vegetative development:parallel studies of CO2assimilation,chlorophyll a fluorescence kinetics O-J-I-P and nitrogen fixation[J]. Physiologia Plantarum,2003,117(4):476-491.
• [39]李国防,王荣花,王志博,刘颖娇,张雯,梁俊,赵政阳,郭延平.NaHSO3对苹果光合作用和叶绿素荧光的影响[J].西北农业学报,2015,24(5):75-80.LI Guofang,WANG Ronghua,WANG Zhibo,LIU Yingjiao,ZHANG Wen,LIANG Jun,ZHAO Zhengyang,GUO Yanping.Effect of NaHSO3on photosynthesis and chlorophyll fluorescence in apple[J]. Acta Agriculturae Boreali-occidentalis Sinica,2015,24(5):75-80.
• [40] WANG H W,SHEN Y G. How bisulfite enhances photosynthesis[J]. Journal of Plant Physiology and Molecular Biology,2002,28(4):247-252.
• [41]陈功亮,罗学刚.铀胁迫对酸模叶绿素荧光特性和酶活性的影响[J].环境科学与技术,2015,38(3):38-43.CHEN Gongliang,LUO Xuegang. Effect of uranium stress on chlorophyll fluorescence characteristics and enzyme activity of Rumex acetosa L.[J]. Environmental Science&Technology,2015,38(3):38-43.
• [42] FOYER C H,NOCTOR G. Oxidant and antioxidant signalling in plants:a re-evaluation of the concept of oxidative stress in a physiological context[J]. Plant,Cell&Environment,2005,28(8):1056-1071.
• [43]裴斌,张光灿,张淑勇,吴芹,徐志强,徐萍.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报,2013,33(5):1386-1396.PEI Bin,ZHANG Guangcan,ZHANG Shuyong,WU Qin,XU Zhiqiang,XU Ping. Effects of soil drought stress on photosynthetic characteristics and antioxidant enzyme activities in Hippophae rhamnoides Linn. seedings[J]. Acta Ecologica Sinica,2013,33(5):1386-1396.
• [44]袁远爽,肖娟,胡艳.模拟酸雨对白筋叶片抗氧化酶活性及叶绿素荧光参数的影响[J].植物生理学报,2014,50(6):758-764.YUAN Yuanshuang,XIAO Juan,HU Yan. Effects of simulated acid rain on antioxidant enzyme activities and chlorophyll fluorescence paramenters in leaves of Acanthopanax trifoliatus[J].Plant Physiology Journal,2014,50(6):758-764.
• [45]查倩,奚晓军,蒋爱丽,田益华,黄健.高温胁迫对葡萄幼树叶绿素荧光特性和抗氧化酶活性的影响[J].植物生理学报,2016,52(4):525-532.ZHA Qian,XI Xiaojun,JIANG Aili,TIAN Yihua,HUANG Jian.Effects of heat stress on chlorophyll fluorescence characteristics and antioxidant activity in grapevines(Vitis vinifera L. cv. Xiahei)[J]. Plant Physiology Journal,2016,52(4):525-532.
• [46] KACHARAVA N,CHKHUBIANISHVILI E,BADRIDZE G,CHANISHVILI S,MAZANISHVILI L. Antioxidant response of some Georgian wheat species to simulated acid rain[J]. Australian Journal of Crop Science,2013,7(6):770-776.
• [47]郭慧媛,马元丹,王丹,左照江,高岩,张汝民,王玉魁.模拟酸雨对毛竹叶片抗氧化酶活性及释放绿叶挥发物的影响[J].植物生态学报,2014,38(8):897-904.GUO Huiyuan,MA Yuandan,WANG Dan,ZUO Zhaojiang,GAO Yan,ZHANG Rumin,WANG Yukui. Effects of simulated acid rain on the activity of antioxidant enzyme and the emission of induced green leaf volatiles in Phyllostachys pubescens[J]. Chinese Journal of Plant Ecology,2014,38(8):897-904.
• [48] CHEN J,WANG W H,LIU T W,WU F H,ZHENG H L. Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain[J]. Plant Physiology and Biochemistry,2013,64(5):41-51.
• [49] VELIKOVA V,YORDANOV I,EDREVA A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants:protective role of exogenous polyamines[J]. Plant Science,2000,151(1):59-66.
• [50] YU J Q,YE S F,HUANG L F. Effects of simulated acid precipitation on photosynthesis,chlorophyll fluorescence,and antioxidative enzymes in Cucumis sativus L.[J]. Photosynthetica,2002,40(3):331-335.