刘义存;黄天启;林顺权;

• 1:仲恺农业工程学院园艺园林学院
• 2:华南农业大学园艺学院

摘要(Abstract)：

【目的】建立野生种枇杷茎段愈伤组织诱导植株再生的离体培养技术体系。【方法】以枇杷属植物的2个野生种、1个属间杂交后代为试材,采用茎段为外植体,在不同植物生长调节剂的组合配比培养条件下,开展野生枇杷茎段诱导愈伤组织以及愈伤组织诱导出不定芽的植株再生研究。【结果】最适合野生种枇杷茎段愈伤组织诱导植株再生的培养基配方为:MS+6-BA 1.0 mg·L~(-1)+TDZ 0.1 mg·L~(-1)+NAA 0.1 mg·L~(-1),如栎叶枇杷茎段愈伤组织诱导植株再生,其愈伤率、丛芽率和成苗率均达到100%,不定芽数最多可达38.75个;但对于属间杂交后代的石斑木×台湾枇杷最适合茎段愈伤组织诱导植株再生的培养基配方为:MS+6-BA 1.0 mg·L~(-1)+NAA 0.1 mg·L~(-1),其丛芽率和芽苗数量分别为100%和48个,成苗率也为100%;在众多的生长调节剂中,TDZ对野生枇杷的愈伤组织诱导再分化或脱分化是比较敏感的,高浓度的TDZ虽有利于愈伤组织诱导再分化不定芽,但不利于成苗。【结论】研究得出枇杷属植物以及属间杂交后代茎段愈伤诱导植株再生的共性规律和种间的差异性,建立起枇杷属植物野生种离体再生系统,简化植株再生诱导的步骤和缩短植株再生诱导的时间,能在短时间内产生一定数量的组培苗,为枇杷属种质资源研究积累了新的资料。

关键词(KeyWords)： 枇杷属植物;野生种;茎段;离体培养;愈伤组织;植株再生

Abstract:

Keywords:

基金项目(Foundation): 广东省科技厅科技基础条件建设项目(2014A030304057,2015A030303015);; 广州市科技创新委资助华南农业大学(亚热带农业生物资源保护与利用)国家重点实验室项目(201504010028)

作者(Author): 刘义存;黄天启;林顺权;

Email:

参考文献(References)：

• [1]杜丰玉,陈钧,宣寒,任晓峰,郭海燕.RP-HPLC测定枇杷叶中熊果酸和齐墩果酸的含量[J].中成药,2006,28(12):1790-1793.DU Fengyu,CHEN Jun,XUAN Han,REN Xiaofeng,GUO Hai-yan.RP-HPLC determination of content of ursolic acid and olea-nolic acid from loquat leaf[J].Chinese Traditional Patent Medi-cine,2006,28(12):1790-1793.
• [2]郭宇,吴松吉,朴惠善.枇杷叶的化学成分及药理活性研究进展[J].时珍国医国药,2006,17(6):928-930.GUO Yu,Wu Songji,PIAO Huishan.Research progress on chemi-cal constituents and pharmacological activities of loquat leaf[J].Lishizhen Medicine and Materia Medica Research,2006,17(6):928-930.
• [3]叶广亿,李书渊,陈艳芬,韩秀奇,梁钻芬,徐结昕,代旻,梁婷.枇杷叶不同提取物的止咳化痰平喘作用比较研究[J].中药药理与临床,2013,29(2):100-102.YE Guangyi,LI Shuyuan,CHEN Yanfen,HAN Xiuqi,LIANGZuanfen,XU Jiexin,DAI min,LIANG Ting.Comparative study ofdifferent extracts of loquat leaf in antitussive and expectorant andantiasthmatic effects[J].Pharmacology and Clinics of Chinese Ma-teria Medica,2013,29(2):100-102.
• [4]林顺权,杨向晖,刘成明,胡又厘,何业华,胡桂兵,张海岚,何小龙,刘月学,刘宗莉.中国枇杷属植物的自然地理分布[J].园艺学报,2004,31(5):569-573.LIN Shunquan,YANG Xianghui,LIU Chengming,HU Youli,HEYehua,HU Guibing,ZHANG Hailan,HE Xiaolong,LIU Yuexue,LIU Zongli.Natural geographical distribution of genus Eriobotrya plants in China[J].Acta Horticulturae Sinica,2004,31(5):101-104.
• [5]杨向晖,格拉贝,林顺权,胡又厘,何业华,阮氏锦绒,刘月学,胡桂兵,刘成明.枇杷属植物种类数及东南亚原产枇杷种类[J].果树学报,2005,22(1):55-59.YANG Xianghui,Glakpe Kodjo,LIN Shunquan,HU Youli,HEYehua,Nguyen Thi Cam Nhung,LIU Yuexue,HU Guibing,LIUChengming.Taxa of plants of genus Eriobotrya around the worldand native to South-eastern Asia[J].Journal of Fruit Science,2005,22(1):55-59.
• [6]王云生,胡又厘,何小龙,杨向晖,田宗伟,林顺权.中国部分野生普通枇杷资源调查分析[J].福建果树,2012(4):11-15.WANG Yunsheng,HU Youli,HE Xiaolong,YANG Xianghui,TIAN Zhongwei,LIN Shunquan.Investigation report on wild andsemi-wild germplasm of loquatin China[J].Fujian Fruits,2012(4):11-15.
• [7]黄爱萍,郑少泉,胡文舜,张立杰,谢丽雪,陈秀妹,张小艳.生物技术在枇杷种质资源保存与利用的研究进展[J].福建农业学报,2008,23(4):457-461.HUANG Aiping,ZHENG Shaoquan,HU Wenshun,ZHANG Li-jie,XIE Lixue,CHEN Xiumei,ZHANG Xiaoyan.Recent advanc-es in biotechnology research of loquat(Eriobotrya Joponica Lindl.)germplasm.[J].Fujian Journal of Agricultural Sciences,2008,23(4):457-461.
• [8]杨永青,陈光禄,唐道一.枇杷实生苗的茎尖培养[J].植物生理学通讯,1982(2):39-40.YANG Yongqing,CHEN Guanglu,TANG Daoyi.Shoot tip cultureof loquat seedling[J].Plant Physiology Communications,1982(2):39-40.
• [9]杨永青,陈光禄,唐道一.枇杷茎尖培养与增殖的研究[J].园艺学报,1983,10(2):79-86.YANG Yongqing,CHEN Guanglu,TANG Daoyi.A study on tis-sue culture and inducing proliferation from shoot apexes of loquattree[J].Acta Horticulturae Sinica,1983,10(2):79-82.
• [10]陈振光,林顺权,林庆良.枇杷胚乳培养获得植株试验初报[J].福建农学院学报,1983,12(4):343-344.CHEN Zhenguang,LIN Shunquan,LIN Qinggang.A preliminaryreport on the experiment of the endosperm culture of loquat[J].Journal of Fujian Agriculture and Forestry University,1983,12(4):343-344.
• [11]林顺权.枇杷胚乳离体培养获得植株的研究[J].福建农学院学报(自然科学版),1985,14(2):117-125.LIN Shunquan.Studies on the plant of loquat endosperm in vitro culture[J].Journal of Fujian Agriculture and Forestry University(Natrual Science Edition),1985,14(2):117-125.
• [12]林顺权,陈振光.枇杷原生质体植株再生[J].园艺学报,1996,23(4):313-318.LIN Shunquan,CHEN Zhenguang.Plant regeneration from proto-plasts of loquat[J].Acta Horticulturae Sinica,1996,23(4):313-318.
• [13]林顺权.枇杷胚胎发生的观察[J].福建农学院学报(自然科学版),1992,21(1):67-71.LIN Shunquan.Some observations on the embryogenesis in loquat[J].Journal of Fujian Agriculture and Forestry University(NatrualScience Edition),1992,21(1):67-71.
• [14]彭晓军,王永清.枇杷胚乳愈伤组织诱导和不定芽发生的研究[J].四川农业大学学报,2002,20(3):228-231.PENG Xiaojun,WNG Yongqing.Callus induction and plant re-generation from endosperm of loquat[J].Journal of Sichuan Agri-cultural University,2002,20(3):228-231.
• [15]滕世云,陈惠民.枇杷胚状体的诱导和植株再生[J].园艺学报,1986,13(4):245-249.TENG Shiyun,CHEN Huimin.Induction of somatic embryogene-sis and plant regeneration in loquat[J].Acta Horticulturae Sinica,1986,13(4):245-249.
• [16]沈庆斌,赖钟雄,蔡汉权,陈义挺,赖呈纯.枇杷幼胚培养与体胚诱导植株再生[J].江西农业大学学报,2005,27(3):379-384.SHEN Qingbin,LAI Zhongxiong,CAI Hanquan,CHEN Yiting,LAI Chengchun.In vitro culture of immature embryos and plantregeneration via somatic embryogenesis in loquat[J].Acta Agricul-turae Universitatis Jiangxiensis,2005,27(3):379-384.
• [17]杨凤玲,林顺权.枇杷茎尖组织培养初代培养物的建立[J].中国南方果树,2006,34(6):36-38.YANG Fengling,LIN Shunquan.Establishment of the first genera-tion of in vitro culture from loquat stem apex[J].South ChinaFruits,2006,34(6):36-38.
• [18]王芳,吕洪飞,徐红霞,杨勇,严成其,汪志平.枇杷幼叶组织培养与植株再生研究[J].生物技术通讯,2011,22(1):53-56.WANG Fang,LüHongfei,XU Hongxia,YANG Yong,YAN Chengqi,WANG Zhiping.Plantlet regeneration from leaves of Eriobotrya japonica Lindl.[J].Lettersin Biotechnology,2011,22(1):53-56.
• [19]陶炼,潘翠萍,谢红江,王永清,杨文渊,杨芩,李靖,陈栋,涂美艳,刘伟,江国良.三倍体枇杷茎尖培养与植株再生[J].热带作物学报,2014,35(11):2249-2254TAO Lian,PAN Cuiping,XIE Hongjiang,WANG Yongqing,YANG Wenyuan,YANG Qin,LI Jing,CHEN Dong,TU Meiyan.LIU Wei,WANG Guoliang.Plant regeneration from shoot tips intriploid loquat[J].Chinese Journal of Tropical Crops,2014,35(11):2249-2254.
• [20]万志刚,宋卫平,顾福根,朱明德,汪洋.良种白沙枇杷“冠玉”的组织培养和快繁技术研究[J].苏州大学学报(自然科学版),2000,16(4):89-92.WAN Zhigang,SONG Weiping,GU Fugen,ZHU Mingde,WANGYang.Study on tissue culture and rapid progagation of Eriobotrya japonica Lindl.[J].Journal of Suzhou University(Natural Sci-ence),2000,16(4):89-92.
• [21]林庆良,林顺权,陈振光.栎叶枇杷原生质体的分离与培养[J].福建农学院学报(自然科学版),1994,23(1):26-29.LIN Qingliang,LIN Shunquan,CHEN Zhenguang.Isolation andculture of protoplasts in Eriobotrya prinades[J].Journal of FujianAgriculture and Forestry University(Natural Science Edition),1994,23(1):26-29.
• [22]张志珂,陈彬,杨凤玲,林顺权.枇杷野生种的种质资源离体保存初探[J].福建果树,2008(3):28-32.ZHANG Zhike,CHEN bin,YANG Fengling,LIN Shunquan.Pre-liminary studies on germplasm preservation in vitro of Eriobotrya[J].Fujian Fruits,2008(3):28-32.
• [23]刘义存,王淑群,黄天启,林顺权.应用于枇杷属植物超低温保存中的程序降温仪参数调整研究[J].园艺学报,2015,42(增刊):2646.LIU Yicun,WANG Shuqun,HUANG Tianqi,LIN Shunquan.Study on the parameter adjustment of the temperature pro-grammed instrument used in the cryopreservation of loquat[J].Acta Horticulturae Sinica,2015,42(Suppl.):2646.
• [24]刘义存,黄天启,林顺权.枇杷属植物野生种的常温离体保存研究[J].中国南方果树,2016,45(4):95-98.LIU Yicun,HUANG Tianqi,LIN Shunquan.The study on preser-vation in vitro of loquats germplasm under room temperature[J].South China Fruits,2016,45(4):95-98.
• [25]刘义存,岑伟仪,黄天启,林玉霜,张志珂,高用顺,林顺权.枇杷属植物茎尖离体培养及褐化抑制研究[J].果树学报,2014,31(3):415-422.LIU Yicun,CEN Weiyi,HUANG Tianqi,LIN Yushuang,ZHANGZhike,GAO Yongshun,LIN Shunquan.Study on shoot tip in vitro culture and inhibit-browning of loquats[J].Journal of Fruit Sci-ence,2014,31(3):415-422.
• [26]刘义存,黄天启,林顺权.枇杷属野生植物种胚离体培养和植株再生研究[J].亚热带植物科学,2016,45(2):112-116.LIU Yicun,HUANG Tianqi,LIN Shunquan.Studies on in vitro culture and regeneration of embryos in wild sepieces of Eriobotrya plants[J].Subtropical Plant Science,2016,45(2):112-116.
• [27]刘翠琼,汤浩茹,罗娅.不同基因型梨叶片离体培养和植株再生[J].园艺学报,2005,32(6):1080-1083.LIU Cuiqiong,TANG Haoru,LUO Ya.Leaf culture and plantletregeneration of pears with different genotypes[J].Acta Horticul-turae Sinica,2005,32(6):1080-1083.
• [28]徐芳杰,张学英,施春晖,王晓庆,奚晓军,蒋爽,陈荣.单核枇杷形成的影响因素研究[J].果树学报,2016,33(6):676-685.XU Fangjie,ZHANG Xueying,SHI Chunhui,WANG Xiaoqing,XI Xiaojun,JIANG Shuang,CHEN Rong.Study on the influencefactors of single-core formation of loquat seedlings[J].Journal ofFruit Science,2016,33(6):676-685.