屈田田;张剑侠;骆强伟;王跃进;

• 1:西北农林科技大学园艺学院·旱区作物逆境生物学国家重点试验室·农业部西北地区园艺作物生物学与种质创制重点试验室
• 2:新疆维吾尔自治区葡萄瓜果研究所

摘要(Abstract)：

【目的】探究亲本基因型对胚挽救的影响,从中选出无核葡萄新单株,为今后无核葡萄新品系的选育提供新材料。【方法】以8个无核葡萄品种为母本,以抗寒抗病的中国野生葡萄等做父本,围绕无核抗性的育种目标设计9个杂交组合,对杂种进行胚珠培养,成苗后进行温室移栽炼苗,并对后代进行杂种鉴定和辅助选择,最后移栽至大田。【结果】通过胚挽救技术获得32个胚挽救株系,394株幼苗。利用无核基因探针GLSP1、分子标记SCF27和SCC8对杂种株系进行分子标记检测,杂种株系中检测出无核特异性条带的分别为11、18、21个株系。其中有17个株系在SCF27和SCC8标记扩增下都出现了无核性状的特异性条带。综合3种标记对杂交子代株系的检测结果,有28个株系被初步确认为携带标记的无核株系。【结论】以‘火焰无核’‘爱神玫瑰’和‘无核白鸡心’为母本的组合成苗率相对较高,以‘底莱特’和‘红无籽露’为母本的组合具有较高的胚萌发率,较适宜做母本。在杂交育种时母本的选择起关键作用,父本对胚挽救的效果也会有一定影响。

关键词(KeyWords)： 无核葡萄;抗病性;抗寒性;胚挽救育种;分子标记辅助选择

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业体系建设专项基金(CARS-30-yz-7)

作者(Author): 屈田田;张剑侠;骆强伟;王跃进;

Email:

DOI: 10.13925/j.cnki.gsxb.20160250

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