郭金丽;刘欢;梁爽;朱冠宇;白杨;李连国;

• 1:内蒙古农业大学农学院

摘要(Abstract)：

【目的】探讨草莓果实衰老过程中超微弱发光(ultraweak luminescence,UWL)与活性氧的关系。【方法】以‘红颜’草莓果实为试材,采用H_2O_2处理和茶多酚处理草莓果实,对比分析促进和清除活性氧条件下草莓果实衰老过程中UWL和活性氧的变化及2者之间的关系。【结果】在草莓果实衰老过程中,H_2O_2处理、茶多酚处理和对照的脂氧合酶(LOX)活性和过氧化氢(H_2O_2)含量均先上升后下降,整体上升;超氧阴离子(O_2^)产生速率、丙二醛(MDA)含量和相对电导率均持续上升;H_2O_2处理和对照的UWL均下降、茶多酚处理的UWL上升。在整个果实衰老过程中,H_2O_2处理的O_2)产生速率、丙二醛(MDA)含量和相对电导率均持续上升;H_2O_2处理和对照的UWL均下降、茶多酚处理的UWL上升。在整个果实衰老过程中,H_2O_2处理的O_2^产生速率、MDA含量和相对电导率均高于对照,茶多酚处理后3者均低于对照;H_2O_2处理的UWL强度均低于对照,茶多酚处理的UWL强度均高于对照。【结论】在草莓果实衰老过程中,UWL强度随着活性氧水平的积累而下降;促进活性氧情况下加剧了草莓果实活性氧上升积累,同时加剧了UWL强度下降;清除活性氧则缓减了活性氧上升积累,同时缓减了UWL强度下降。以上结果暗示活性氧并不是引发UWL的直接来源,而应是通过影响其他细胞结构或物质代谢从而间接影响UWL。

关键词(KeyWords)： 草莓果实;衰老;活性氧调控;超微弱发光;活性氧

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31260455)

作者(Authors): 郭金丽;刘欢;梁爽;朱冠宇;白杨;李连国;

DOI: 10.13925/j.cnki.gsxb.20160175

参考文献(References)：

• [1]POPP F A,LI K H,MEI W P,GALLE M,NEUROHR R.Physical aspects of biophotons[J].Experientia,1988:44(7):576-85.
• [2]POPP F A,LI K H,GU Q.Recent advances in biophoton research and its applications[M].World Scientific Pub Co Inc.,1992:1-46.
• [3]袁佐清.生物超微弱发光研究进展[J].安徽农业科学,2008,36(8):3092-3094.YUAN Zuoqing.Research progress on ultraweak bioluminescence[J].Journal of Anhui Agricultural Science,2008,36(8):3092-3094.
• [4]GURWITSCH A.Die natur des spezifischen erregers der zellteilung[J].Development Genes and Evolution,1923,100(1):11-40.
• [5]王畅,蒋礼林,王乐新,朱文霞.奶牛血清微弱发光的分析与应用研究[J].江苏农业科学,2013,41(2):187-189.WANG Chang,JIANG Lilin,WANG Lexin,ZHU Wenxia.Analysis and application of ultraweak bioluminescence of serum in dairy cow[J].Jiangsu Aricultural Science,2013,41(2):187-189.
• [6]岳霞丽,刘永红,胡先文,董元彦.水华鱼腥藻的超弱发光研究[J].光谱实验室,2008,25(4):673-676.YUE Xiali,LIU Yonghong,HU Xianwen,DONG Yuanyan.The study of ultraweak luminescence of Anabaena Flos-Aquae[J].Chinese Journal of Spectroscopy Laboratory,2008,25(4):673-676.
• [7]胡献明,潘建伟,陈虹,朱睦元.铝胁迫诱导大麦根尖细胞超微弱发光的变化[J].浙江大学学报,2002,28(4):383-386.HU Xianming,PAN Jianwei,CHEN Hong,ZHU Muyuan.Aluminum-induced ultraweak luminescence changes in root-tip cells of barley[J].Journal of Zhejiang University,2002,28(4):383-386.
• [8]侯仙慧,廖祥儒,李颖,张晓晴,卜文娟,贾燕,李光.苋菜种子萌发过程的超微弱发光及其机理研究[J].种子,2004,23(7):23-27.HOU Xianhui,LIAO Xiangru,LI Ying,Zh ANG Xiaoqing,BU Wenjuan,JIA Yan,LI Guang.Ultraweak biophoton emission and its mechanism during seed germination of Amaranthus hypochondriacus[J].Seed,2004,23(7):23-27.
• [9]刘红梅,廖祥儒,吴立峰,蒋继志.热休克对小麦未成熟种子萌发、生物发光和抗氧化酶活性的影响[J].食品与生物技术学报,2006,25(3):75-78.LIU Hongmei,LIAO Xiangru,WU Lifeng,JIANG Jizhi.Effect of heat shock on biophoton and activities of antioxidant enzymes in immature wheat grains[J].Journal of Food Science and Biotechnology,2006,25(3):75-78.
• [10]林桂玉,黄在范,张翠华,郑成淑.菊花花芽分化期超微弱发光及生理代谢的变化[J].园艺学报,2008,35(12):1819-1824.LIN Guiyu,HUANG Zaifan,ZHANG Cuihua,ZHENG Chengshu.Changes in ultraweak luminescence intensity and physiological metabolism of chrysanthemum during floral differentiation[J].Acta Horticulturae Sinica,2008,35(12):1819-1824.
• [11]接玉玲,赵海洲,张伟,杨洪强,李德全,束怀瑞.甜菜碱对干旱胁迫下湖北海棠超微弱发光及抗氧化能力的影响[J].应用生态学报,2006,17(12):2394-2398.JIE Yuling,ZHAO Haizhou,ZHANG Wei,YANG Hongqiang,LI Dequan,SHU Huairui.Effects of glycinebeta ine on ultraweak luminescence and anti-oxidative capability of Malus hupehensis under drought stress[J].Chinese Journal of Applied Ecology,2006,17(12):2394-2398.
• [12]赵丹莹,生吉萍,丁洋,申琳,范蓓,刘灿.超微弱发光用于番茄果实冷害发生程度的无损监测[J].光谱学与光谱分析,2010,30(9):2493-2495.ZHAO Danying,SHENG Jiping,DING Yang,SHEN Lin,FAN Bei,LIU Can.Nondestructive examination of tomato chilling injury by ultraweak luminescence[J].Spectroscopy and Spectral Analysis,2010,30(9):2493-2495.
• [13]张菊平,张兴志,巩振辉.超微弱发光在蔬菜研究中的应用[J].中国农学通报,2006,22(1):220-222.ZHANG Juping,ZHANG Xingzhi,GONG Zhenhui.Application of ultraweak bioluminescence in vegetable research[J].Chinese Agricultural Science Bulletin,2006,22(1):220-222.
• [14]樊秀彩,关军锋,张继澍,李广敏.草莓采后微粒体膜Ca2+-ATPase活性与膜脂过氧化水平[J].园艺学报,2003,30(1):15-18.FAN Xiucai,GUAN Junfeng,ZHANG Jishu,LI Guangmin.Microsomal membrane Ca2+-ATPase activity and lipid peroxidation level during senescence of postharvest strawberry fruit[J].Acta Horticulturae Sinica,2003,30(1):15-18.
• [15]陈学红,郑永华,杨震峰,马素娟,冯磊,王薛修.高氧处理对草莓果实采后活性氧代谢和腐烂的影响[J].南京农业大学学报,2005,28(1):99-102.CHEN Xuehong,ZHENG Yonghua,YANG Zhenfeng,MA Sujuan,FENG Lei,WANG Xuexiu.Effects of high oxygen treatments on active oxygen metabolism and fruit decay in postharvest strawberry fruit[J].Journal of Nanjing Agricultural University,2005,28(1):99-102.
• [16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2004:261-262.LI Hesheng.The physiological experimental principle and technology of plant[M].Beijing:Advanced Education Press,2004:261-262.
• [17]李玲.植物生理学模块实验指导[M].北京:科学出版社,2009:83.LI Ling.The physiological module experimental guide of plant[M].Beijing:Science Press,2009:83.
• [18]顾采琴,朱冬雪,李棋.草莓果实采后NAD激酶活性与NAD(H)、NADP(H)含量及活性氧代谢的关系[J].中国农业科学,2007,40(2):352-357.GU Caiqin,ZHU Dongxue,LI Qi.Relationship between NAD kinase and NAD(H),NADP(H)and active oxygen during ripening and senescence of postharvested strawberry fruit[J].Scientia Agricultura Sinica,2007,40(2):352-357.
• [19]张新华,杨洪强.植物的超微弱发光[J].山东农业大学学报,2003,34(4):605-608.ZHANG Xinhua,YANG Hongqiang.Ultraweak bioluminescence in plant[J].Journal of Shandong Aricultural University,2003,34(4):605-608.
• [20]刘高峰,杨洪强,张伟,马方放.水杨酸对湖北海棠活性氧代谢及超微弱发光的影响[J].园艺学报,2006,33(1):118-121.LIU Gaofeng,YANG Hongqiang,ZHANG Wei,MA Fangfang.Effects of salicylic acid on the active oxygen metabolism and ultraweak luminescence of Malus hupehensis Rehd.[J].Acta Horticulturae Sinica,2006,33(1):118-121.
• [21]张新华,杨洪强.植物叶绿体和线粒体的超微弱发光[J].植物生理学通讯,2004,40(1):111-114.ZHANG Xinhua,YANG Hongqiang.Ultraweak bioluminescence of chloroplast and mitochondria in plants[J].Plant Physiology Communication,2004,40(1):111-114.
• [22]郭金丽,朱冠宇,刘艳,陈贵华,刘欢,李连国.草莓果实衰老过程中超微弱发光的变化及与活性氧的关系[J].北方园艺,2016,38(11):1-4.GUO Jinli,ZHU Guanyu,LIU Yan,CHEN Guihua,LIU Huan,LI Lianguo.The dynamic variation of UWL and reactive oxygen during the ageing process of strawberry fruit[J].Northern Horticulture,2016,38(11):1-4.