刘有春;鄂辉邦;刘成;王兴东;杨艳敏;孙斌;张舵;袁兴福;魏永祥;

• 1:辽宁省果树科学研究所
• 2:青海省互助县边滩良种场
• 3:辽宁省农业科学院

摘要(Abstract)：

【目的】探讨越橘果实糖酸性状在后代中的变异和遗传倾向,为越橘品质育种中亲本的选配提供科学依据。【方法】以越橘半同胞系杂交组合为试材,连续2 a利用高效液相色谱技术对亲本及杂交组合后代成熟果实中可溶性糖及有机酸的组分和含量进行检测和分析。【结果】1)糖、酸组分和总糖、总酸平均含量在绝大多数组合中低于或接近于亲中值,存在一定程度的衰退变异;总酸变异系数大于总糖,各组合均分离出一定比例的超高亲单株。2)杂种后代的糖含量呈连续变异,趋近于正态分布,总糖的广义遗传力(H~2)和遗传传递力(Ta)平均值分别是0.74和0.93,主要表现为多基因控制并以加性效应为主的数量遗传;杂种后代枸橼酸和总酸呈偏正态或多峰分布,可能为由主效基因和多基因共同控制的数量性状,H~2平均值分别为0.69和0.72,变异主要来自遗传效应,Ta分别为0.94和0.86,表现为受加性效应影响大,而酒石酸H~2和Ta平均值分别是0.37和0.74,表明酒石酸的遗传效应小,受环境和非加性效应影响大,不能稳定遗传。【结论】越橘果实糖酸含量在杂交后代中均存在衰退变异,糖含量是以加性遗传效应为主且由多基因控制的数量性状,后代糖含量受低糖亲本影响大;越橘果实酸含量受父本影响,其中枸橼酸是主要有机酸组分,能较稳定遗传,而酒石酸受非加性效应影响大,不能稳定遗传。

关键词(KeyWords)： 越橘;半同胞系杂交组合;糖;酸;广义遗传力;遗传传递力

Abstract:

Keywords:

基金项目(Foundation): 辽宁省博士启动基金(201501118);; 国家科技支撑计划(2013BAD02B04-05)

作者(Author): 刘有春;鄂辉邦;刘成;王兴东;杨艳敏;孙斌;张舵;袁兴福;魏永祥;

Email:

DOI:

参考文献(References)：

• [1]HOU D X.Potential mechanisms of cancer chemoprevention by anthocyanins[J].Current Molecular Medicine,2003,3(2):149-159.
• [2]PRIOR R L,CAO G H,MARTIN A,SOFIC E,MCEWEN J,O'BRIEN C,LISCHNER N,EHLENFELDT M,KALT W,KREWER G.Antioxidant capacity as influenced by total phenolic and anthocyanin content,maturity,and variety of Vaccinium species[J].Journal of Agricultural and Food Chemistry,1998,46(7):2686-2693.
• [3]ROWLAND L J,ALKHAROUF N,DARWISH O,OGDEN E L,POLASHOCK J J,BASSIL N V,MAIN D.Generation and analysis of blueberry transcriptome sequences from leaves,developing fruit,and flower buds from cold acclimation through deacclimation[J].BMC Plant Biology,2012,12:46.
• [4]李亚东,刘海广,张志东,吴林.我国蓝莓产业现状和发展趋势[J].中国果树,2008(6):67-71.LI Yadong,LIU Haiguang,ZHANG Zhidong,WU Lin.Current situation and development trend of blueberry industry in China[J].China Fruits,2008(6):67-71.
• [5]李亚东.中国蓝莓产业发展报告(种植篇)[OL].蓝莓中国微信公共平台,2015.LI Yadong.A report on blueberry industry development of China planting chapter[OL].Blueberry China We Chat Public Platform,2015.
• [6]LIU Y C,LIU S,LIU D C,WEI Y X,LIU C,YANG Y M,TAO C G,LIU W S.Exploiting EST databases for the development and characterization of EST-SSR markers in blueberry(Vaccinium)and their cross-species transferability in Vaccinium spp[J].Scientia Horticulturae,2014,176:319-329.
• [7]ESTI M,MESSIA M C,SINESIO F,NIOTRA A,CONTE L,LANOTTE E,PALLESCHI G.Quality evaluation of peaches and nectarines by electrochemical and multivariate analyses:relationships between analytical measurements and sensory attributes[J].Food Chemistry,1997,60(4):659-666.
• [8]陈昌文,张佳卉,曹珂,朱更瑞,方伟超,王新卫,王力荣.桃地方品种果实糖酸主要组分的遗传关联分析[J].果树学报,2015,32(6):1036-1046.CHEN Changwen,ZHANG Jiahui,CAO Ke,ZHU Gengrui,FANG Weichao,WANG Xinwei,WANG Lirong.Genetic association of sugar and acid main constituents of China landrace peach(Prunus persica L.)[J].Journal of Fruit Science,2015,32(6):1036-1046.
• [9]EHLENFELDT M K,MEREDITH F I,BALLINGTON J R.Unique organic acid profile of rabbiteye vs.highbush blueberries[J].Hort Science,1994,29(4):321-323.
• [10]AYAZ F A,KADIOGUL A,BERTOFT E,ACAR C,TURNA I.Effect of fruit maturation on sugar and organic acid composition in two blueberries(Vaccinium arctostaphylos and V.myrtillus)native to Turkey[J].New Zealand Journal of Crop and Horticultural Science,2001,29(2):137-141.
• [11]张春雨,李亚东,刘海广,张志东,林吴,王晶莹.越橘糖酸组分及含量分析[J].园艺学报,2010,37(4):619-624.ZHANG Chunyu,LI Yadong,LIU Haiguang,ZHANG Zhidong,LIN Wu,WANG Jingying.Analysisfor components and contents of sugars and organic acids in blueberryfruits[J].Acta Horticulturae Sinica,2010,37(4):619-624.
• [12]刘有春,陶承光,魏永祥,刘成,王兴东,刘威生,杨艳敏.越橘果实糖酸含量和不同发育阶段的变化及其与叶片中可溶性糖含量的相关关系[J].中国农业科学,2013,46(19):4110-4118.LIU Youchun,TAO Chengguang,WEI Yongxiang,LIU Cheng,WANG Xingdong,LIU Weisheng,YANG Yanmin.Fruit sugar and acid content,variation at different fruit development stages and their relationship with leaf soluble sugar content of blueberry[J].Scientia Agricultura Sinica,2013,46(19):4110-4118.
• [13]刘有春,魏永祥,王兴东,刘成,蒋明三,张舵,袁兴福,陶承光.南高丛越橘品种‘Sapphire’和北高丛品种‘Berkeley’正反交后代果实糖酸组分含量的遗传倾向[J].中国农业科学,2014(24):4878-4885.LIU Youchun,WEI Yongxiang,WANG Xingdong,LIU Cheng,JIANG Mingsan,ZHANG Duo,YUAN Xingfu,TAO Chengguang.Inheritance tendency of sugar and acid contents in the reciprocal cross progenies’fruits of southern×northernhigh bush blueberry(Vaccinium)[J].Scientia Agricultura Sinica,2014(24):4878-4885.
• [14]魏鑫,刘成,王兴东,杨玉春,孙斌,魏永祥.6个高丛越橘品种低温半致死温度的测定[J].果树学报,2013,30(5):798-802.WEI Xin,LIU Cheng,WANG Xingdong,YANG Yuchun,SUN Bin,WEI Yongxiang.Study on semi-lethal temperatures of six highbush blueberry cultivars[J].Journal of Fruit Science,2013,30(5):798-802.
• [15]张立彬,肖啸,王学东,戴刚.大久保桃自交后代若干性状的变异和遗传倾向[J].果树学报,2004,21(4):308-310.ZHANG Libin,XIAO Xiao,WANG Xuedong,DAI Gang.Variability and inheritance of some characters in self-crossed progeny of Okubo peach cultivar[J].Journal of Fruit Science,2004,21(4):308-310.
• [16]CHEN X S,WU Y,CHEN M X,HE T M,FENG J R,LIANG Q,LIU W,YANG H H,ZHANG L J.Inheritance and correlation of self-compatibility and other yield components in the apricot hybrid F1 populations[J].Euphytica,2006,150(1-2):69-74.
• [17]沈德绪.果树遗传学[M].上海:上海科技出版社,1986.SHEN Dexu.Genetics of fruit tree[M].Shanghai:Shanghai Science and Technology Press,1986.
• [18]崔艳波,陈慧,乐文全,张树军,伍涛,陶书田,张绍铃.‘京白梨’与‘鸭梨’正反交后代果实性状遗传倾向研究[J].园艺学报,2011,28(2):215-224.CUI Yanbo,CHEN Hui,LE Wenquan,ZHANG Shujun,WU Tao,TAO Shutian,ZHANG Shaoling.Studies on genetic tendency of fruit characters in reciprocal crosses generation between‘Jingbaili’and‘Yali’pear cultivars[J].Journal of Fruit Science,2011,28(2):215-224.
• [19]LIANG Z C,YANG C X,YANG J,WU B H,WANG L J,CHENG J S,LI S H.Inheritance of anthocyanins in berries of Vitis vinifera grapes[J].Euphytica,2009,167(1):113-125.
• [20]LIU H F,WU B H,FAN P G,XU H Y,LI S H.Inheritance of sugars and acids in berries of grape(Vitis vinifera L.)[J].Euphytica,2007,153(1-2):99-107.
• [21]WU B H,ZHAO J B,CHEN J,XI H F,JIANG Q,LI S H.Maternal inheritance of sugars and acids in peach(P.persica(L.)Batsch)fruit[J].Euphytica,2012,188(3):333-345.
• [22]许家辉,余东,魏秀清,许玲,蒋际谋,黄金松:枇杷正反交杂种后代果实形状遗传分析[J].云南农业大学学报,2008,23(5):663-667.XU Jiahui,YU Dong,WEI Xiuqing,XU Ling,JIANG Jimou,HUANG Jinsong.Genetic analysis of fruit agronomic haraters in reciprocal hybrid progeny of loquat[J].Journal of Yunnan Agricultural University,2008,23(5):663-667.
• [23]景士西,吴禄平,李宝江.果树遗传变异的遗传特质初探[J].遗传,1995,17(1):40-44.JING Shixi,WU Luping,LI Baojiang.Preiminary study on genetic characteristics of fruitplants[J].Hereditas,1995,17(1):40-44.
• [24]ETIENNE C,ROTHAN C,MOING A,PLOMION C,BODéNèS C,SVANELLA-DUMAS L,COSSON P,PRONIER V,MONET R,DIRLEWANGER E.Candidate genes and QTLs for sugar and organic acid content in peach[Prunus persica(L.)Batsch][J].Theoretical and Applied Genetics,2002,105(1):145-159.
• [25]QUILOT B,GéNARD M,KERVELLA J,LESCOURRET F.Analysis of genotypic variation in fruit flesh total sugar content via an ecophysiological model applied to peach[J].Theoretical and Applied Genetics,2004,109(2):440-449.
• [26]宋润刚,郑永春,路文鹏,李昌禹,沈育杰,李晓红,张宝香,林兴桂.山葡萄种内和种间杂交(F1-F4)代果实酸和糖含量的遗传分析[J].园艺学报,2007,34(4):813-822.SONG Rungang,ZHENG Yongchun,LU Wenpeng,LI Changyu,SHEN Yujie,LI Xiaohong,ZHANG Baoxiang,LIN Xinggui.Hereditary analysis of total fruit acid content and sugar content of progenies(F1-F4)of Vitis amurensis Rupr.via intraspecific or interspecific hybridization[J].Acta Horticulturae Sinica,2007,34(4):813-822.
• [27]路文鹏,宋润刚,李晓红,焦培娟,沈育杰,刘洪章.山葡萄种内杂交后代浆果色素单宁和总酸含量的遗传分析[J].吉林农业大学学报,2003,25(6):629-633.LU Wenpeng,SONG Rungang,LI Xiaohong,JIAO Peijuan,SHEN Yujie,LIU Hongzhang.Genetic analysis of fruit pigment,tannin and aid of progenies of Vitis amurensis via intraspeific hybridization[J].Journal of Jilin Agricultural University,2003,25(6):629-633.
• [28]李宝江,景士西,丁玉英,张景娥.苹果糖酸遗传和选择研究[J].遗传学报,1994,21(2):147-154.LI Baojiang,JING Shixi,DING Yuying,ZHANG Jinge.Study of the iheritance and seletion of sweetness and aidity in apple[J].Acta Genetica Sinica,1994,21(2):147-154.
• [29]刘志,伊凯,王冬梅,杨巍,杨锋,张景娥.富士杂交后代果实内在品质性状的遗传[J].果树学报,2004,21(2):95-102.LIU Zhi,YI Kai,WANG Dongmei,YANG wei,YANG Feng,ZHANG Jing’e.Studies on the fruit internal characteristics inheritance trends of Fuji apple variety crossed progenies[J].Journal of Fruit Science,2004,21(2):95-102.
• [30]郭黄萍,杨盛,张晓伟.库尔勒香梨部分果实性状遗传的研究[J].中国农学通报,2011,27(25):173-177.GUO Huangping,YANG Sheng,ZHANG Xiaowei.Study on the inheritance of some fruit characters in‘Kuerexiangli’prar[J].Chinese Agricultural Science Bulletin,2011,27(25):173-177.
• [31]李俊才,伊凯,刘成,隋洪涛,王家珍.梨果实部分性状遗传倾向研究[J].果树学报,2002,19(2):87-93.LI Juncai,YI Kai,LIU Cheng,SUI Hongtao,WANG Jiazhen.Studies on the trend of inheritance of some charaters of pear fruit[J].Journal of Fruit Science,2002,19(2):87-93.
• [32]王宏伟,王成荣,于淼,戴洪义,王然.梨杂交后代果实主要有机酸遗传动态的研究[J].青岛农业大学学报(自然科学版),2008,25(3):231-235.WANG Hongwei,WANG Chengrong,YU Miao,DAI Hongyi,WANG Ran.Genetic tengeny of organic acid in the pear hybrid progency[J].Journal of Qingdao Agricultural University(Natural Science Edition),2008,25(3):231-235.
• [33]MALIEPAARD C,ALSTON F H,VAN ARKEL G,BROWN L M,CHEVREAU E,DUNEMANN F,EVANS K M,GARDINER S,GUILFORD P,VAN HEUSDEN A W.Aligning male and female linkage maps of apple(Malus pumila Mill.)using multi-allelic markers[J].Theoretical and Applied Genetics,1998,97(1-2):60-73.
• [34]王力荣,朱更瑞,方伟超.中国桃遗传资源[M].北京:中国农业出版社,2012.WANG Lirong,ZHU Gengrui,FANG Weichao.Peach germplasm resources in China[M].Beijing:China Agriculture Press,2012.
• [35]LIEBHARD R,KOLLER B,GIANFRANCESCHI L,GESSLER C.Creating a saturated reference map for the apple(Malus×domestica Borkh.)genome[J].Theoretical and Applied Genetics,2003,106(8):1497-1508.
• [36]孔繁玲.植物数量遗传学[M].北京:中国农业大学出版,2006.KONG Fanling.Quantitative genetics of plant[M].Beijing:China Agricultural University Press,2006.