易龙;卢占军;钟八莲;赖晓桦;陈慈相;

• 1:赣南师范学院生命与环境科学学院
• 2:江西省脐橙工程技术研究中心

摘要(Abstract)：

运用PCR技术以柑橘黄龙病病原亚洲种、非洲种和美洲种特异性引物对赣南脐橙上7个代表性柑橘黄龙病样品进行扩增检测,结合限制性内切酶XbaⅠ对赣南脐橙上的黄龙病病原16S rDNA基因RFLP分析和测定的16SrDNA基因序列同GenBank登录的亚洲种、非洲种和美洲种的相应基因序列进行比对分析。结果表明,在赣南脐橙上检测到黄龙病病原亚洲种,未检测到非洲种和美洲种;赣南脐橙上黄龙病病原16S rDNA基因XbaⅠ酶切具有亚洲种图谱;16S rDNA基因序列比对分析表明7个赣南脐橙上的黄龙病病原间同源性为99.8%~100%,与亚洲种具有98.4%~99.9%的序列同源性,高于与非洲种/美洲种的序列同源性;与亚洲种间遗传距离小于与非洲种/美洲种间的遗传距离;系统聚类分析表明7个赣南脐橙上的黄龙病病原同亚洲种聚类在同一组群,具有较高的同源性和较近的亲缘关系,而与非洲种和美洲种在系统发育树中分属不同的组群,试验结果表明赣南脐橙上感染的黄龙病病原为亚洲种。

关键词(KeyWords)： 赣南脐橙;柑橘黄龙病;16S rDNA;RFLP分析;序列比对

Abstract:

Keywords:

基金项目(Foundation): 江西省青年科学家培养计划(2010DQ02300);; 江西省科技支撑计划项目(2010BNB01402);; 江西省教育厅科技项目(GJJ11585);; 公益性行业(农业)科研专项经费(201003067-02-6)

作者(Author): 易龙;卢占军;钟八莲;赖晓桦;陈慈相;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.06.005

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