何晓薇;王彩虹;田义轲;王辉;

• 1:青岛农业大学园艺学院

摘要(Abstract)：

以24个苹果品种以及来自3个不同杂交组合的60个F1代结果单株为试材,对与苹果果皮颜色性状相关的DNA分子标记进行了研究。通过采用集群分类法(Bulked Segregant Analysis,BSA)对当前定位于苹果基因组图谱LG9上的所有SSR引物的分析,筛选到了一个与苹果果皮红色/非红色性状相关的SSR标记CN444542-156,该标记在红色表型上表现为隐性,在非红色表型上表现为显性。进一步分析表明,该标记对F1代单株性状预测的正确率为93.3%。

关键词(KeyWords)： 苹果;果皮颜色;SSR标记

Abstract:

Keywords:

基金项目(Foundation): 国家“863”计划(2006AA100108-3-5);; 山东省良种工程项目

作者(Author): 何晓薇;王彩虹;田义轲;王辉;

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DOI:

参考文献(References)：

• [1]CHENG F S,WEEDEN N F,BROWN S K.Identification of co-dominant RAPD markers tightly linked to fruit skin color in apple[J].Theor Appl Genet,1996,93:222-227.
• [2]ZHAO Jing,TIAN Yi-ke,WANG Cai-hong,DAI Hong-yi,WANGDong.Identification of RAPD marker linked to the red skin traits ofapples[J].Journal of Fruit Science,2006,23(2):165-168.赵静,田义轲,王彩虹,戴洪义,王东.与苹果果皮红色性状相关的RAPD分子标记的筛选[J].果树学报,2006,23(2):165-168.
• [3]HEMMAT M,WEEDEN N F,MANGANARIS A G,LAWSON D M.Molecular marker linkage map for apple[J].Journal of Heredity,1994,85(1):4-11.
• [4]CONNER P J,BROWN S K,WEEDEN N F.Randomly amplifiedpolymorphic DNA-based genetic linkage maps of three apple culti-vars[J].Am Soc Hortic Sci 1997,122:350-359.
• [5]MALIEPAARD C,ALSTON F H,VAN ARKEL G,BROWN L M,CHEVREAU E,DUNEMANN F,EVANS K M,GARDINER S,GUILFORD P,VAN HEUSDEN A W,JANSE J,LAURENS F,LYNN J R,MANGANARIS A G,DEN NIJS A P M,PERIAM N,RIKKERINK E,ROCHE P,RYDER C,SANSAVINI S,SCHMIDTH,TARTARINI S,VERHAEGH J J,VRIELINK-VAN GINKELM,KING G J.Aligning male and female linkage maps of apple(Malus pumila Mill.)usingmulti-allelicmarkers[J].TheorApplGenet,1998,97:60-73.
• [6]LIEBHARD R,KOLLER B,GIANFRANCESCHI L,GESSLER C.Creating a saturated reference map for the apple(Malus×domesticaBorkh.)genome[J].Theor Appl Genet,2003,106:1497-1508.
• [7]KENIS K,KEULEMANS J.Genetic linkage maps of two apple culti-vars(Malus×domestica Borkh.)based on AFLP and microsatellitemarkers[J].Molecular Breeding,2005,15:205-219.
• [8]SILFVERBERG-DILWORTH E,MATASCI C L,VAN DE WEG WE,VAN KAAUWEN M P W,WALSER M,KODDE L P,SOGLIOV,GIANFRANCESCHI L,DUREL C E,COSTA F,YAMAMOTOT,KOLLER B,GESSLER C,PATOCCHI A.Microsatellite markersspanning the apple(Malus×domestica Borkh.)genome[J].Tree Ge-netics&Genomes,2006,2:202-224.
• [9]TIAN Yi-ke,WANG Cai-hong,ZHANG Ji-shu,DAI Hong-yi,CHUQing-gang.A RAPD marker of apple columnar gene(Co)[J].ActaBot Boreal Occident Sin,2003,23(12):2176-2179.田义轲,王彩虹,张继澍,戴洪义,初庆刚.一个与苹果柱型基因(Co)连锁的RAPD标记[J].西北植物学报,2003,23(12):2176-2179.