高晶晶;冯新新;段春慧;李健花;师忠轩;高付永;汪良驹;

• 1:南京农业大学园艺学院
• 2:徐州市果树研究所

摘要(Abstract)：

【目的】为了探讨5-氨基乙酰丙酸(ALA)在苹果生产上应用的可能性,【方法】以‘红星’和‘富士’苹果为材料,研究了生长季节喷施低质量浓度(0.05~5 mg·L-1)外源ALA对苹果叶片叶绿素相对含量(SPAD)、气体交换参数、叶绿素荧光快速诱导曲线(OJIP)、820 nm光吸收曲线以及果实品质的影响。【结果】0.05~5 mg·L-1ALA均能不同程度地提高苹果叶片SPAD,增加叶片净光合速率(P n)、瞬时羧化效率(P n/Ci)。M-PEA测定结果表明,ALA处理叶片单位面积吸收(ABS/CS)、捕获(TR0/CS)和传递(ET0/CS)的能量以及光合性能指数(PI ABS和PI CS)均有不同程度升高,PSI反应中心的氧化和再还原速率上升,同时以热形式耗散的能量(DI o/CS和DI o/RC)下降,表明低浓度ALA可以显著促进苹果叶片光合电子传递和能量转换。另外,果实成熟时,ALA处理果实的硬度、花青素、可溶性固形物、可溶性糖、可溶性蛋白和维生素C含量显著高于对照,而可滴定酸含量则低于对照。【结论】生长季节喷施低浓度ALA可以提高苹果叶片光合性能,改善果实内外品质。

关键词(KeyWords)： 苹果;5-氨基乙酰丙酸;净光合速率;叶绿素快速荧光;果实品质

Abstract:

Keywords:

基金项目(Foundation): 江苏省农业自主创新基金[CX(10)111]

作者(Author): 高晶晶;冯新新;段春慧;李健花;师忠轩;高付永;汪良驹;

Email:

DOI:

参考文献(References)：

• [1]WANG Liang-ju,JIANG Wei-bing,ZHANG Zhen,YAO Quanhong,MATSUI Hiroyuki,OHARA Hitoshi.Biosynthesis and physiological activities of 5-aminolevulinic acid(ALA)and its potential application in agriculture[J].Plant Physiol Comm,2003,39(3):185-192.汪良驹,姜卫兵,章镇,姚全红,松井弘之,小原均.5-氨基乙酰丙酸生物合成和生理活性及其在农业生产中的潜在应用[J].植物生理学通讯,2003,39(3):185-192.
• [2]WANG Liang-ju,JIANG Wei-bing,HUANG Bao-jian.Promotion of photosynthesis by 5-aminolevulinic acid(ALA)during and after chilling stress in melon seedlings grown under low light condition[J].Acta Hort Sin,2004,31(3):321-326.汪良驹,姜卫兵,黄保健.5-氨基乙酰丙酸对弱光下甜瓜幼苗光合作用和抗冷性的促进效应[J].园艺学报,2004,31(3):321-326.
• [3]WANG Liang-ju,SHI Wei,LIU Hui,LIU Wei-qin,JIANG Weibing,HOU Xi-lin.Effects of exogenous 5-aminolevulinic acid treatment on leaf photosynthesis of pak-choi[J].J Nanjing Agri Univ,2004,27(2):34-38.汪良驹,石伟,刘晖,刘卫琴,姜卫兵,侯喜林.外源5-氨基乙酰丙酸处理对小白菜叶片的光合作用效应[J].南京农业大学学报,2004,27(2):34-38.
• [4]WANG Liang-ju,LIU Wei-qin,SUN Guo-rong,WANG Jian-bo,JIANG Wei-bing,LIU Hui,LI Zhi-qiang,ZHUANG Meng.Effects of 5-aminolevulinic acid on photosynthesis and chlorophyll fluorescence of radish seedlings[J].Acta Bot Boreal-Occident Sin,2005,25(3):488-496.汪良驹,刘卫琴,孙国荣,王建波,姜卫兵,刘晖,李志强,庄猛.ALA对萝卜不同叶位叶片光合作用与叶绿素荧光特性的影响[J].西北植物学报,2005,25(3):488-496.
• [5]KANG Lang,CHENG Yun,WANG Liang-ju.Effects of 5-aminolevulinic acid(ALA)on the photosynthesis and anti-oxidative enzymes activities of the leaves of greenhouse watermelon in summer and winter[J].Acta Bot Boreal-Occident Sin,2006,26(11):2297-2301.康琅,程云,汪良驹.5-氨基乙酰丙酸对秋冬季大棚西瓜叶片光合作用及抗氧化酶活性的影响[J].西北植物学报,2006,26(11):2297-2301.
• [6]SUN Yong-ping,ZHANG Zhi-ping,XU Cheng-xiang,SHEN Chun-miao,GAO Chong,WANG Liang-ju.Effect of ALA on fast chlorophyll fluorescence induction dynamics of watermelon leaves under chilling stress[J].Acta Hort Sin,2009,36(5):671-678.孙永平,张治平,徐呈祥,申春苗,高充,汪良驹.5-氨基乙酰丙酸处理对低温下西瓜叶片快速叶绿素荧光诱导曲线的影响[J].园艺学报,2009,36(5):671-678.
• [7]SU Chang-hong,ZHONG Xiu-li,WANG Dao-long,LI Mao-song.Effects of different chemicals on resistance of tomato seedling to chilling under low light[J].J Natural Disasters,2006,15(6):312-317.苏常红,钟秀丽,王道龙,李茂松.几种化学物质对番茄幼苗耐低温弱光能力的影响[J].自然灾害学报,2006,15(6):312-317.
• [8]WANG Ting,RAO Jing-ping,SONG Yong-ling,ZHANG Hai-yan.ZOU Zhi-rong.Effects of foliar application 5-aminolevulinic acid on post harvest physiological of tomato during cold storage period[J].Acta Bot Boreali-Occidental Sin,2009,18(1):243-247.王婷,饶景萍,宋永令,张海燕,邹志荣.叶面喷施5-氨基乙酰丙酸对番茄果实采后生理指标的影响[J].西北农业学报,2009,18(1):243-247.
• [9]XU Ming,XU Fu-li.Effects of exogenous 5-aminolevulinic acid on the growth and the yield of the tomato in sunlight greenhouse[J].J Northwest A&F Univ:Natural Sci Ed,2008,36(9):126-130.徐铭,徐福利.5-氨基乙酰丙酸对日光温室番茄生长发育和产量品质的影响[J].西北农林科技大学学报:自然科学版,2008,36(9):126-130.
• [10]YIN Lu-lu,YU Xian-chang,WANG Ying-hua,XU Zhen-hang,LI Kun,HAN Dao-jie.Effect of 5-aminolevulinic acid on chillingtolerance in cucumber seedlings[J].Acta Bot Boreal-Occidental Sin,2007,16(4):166-169.尹璐璐,于贤昌,王英华,徐贞杭,李坤,韩道杰.5-氨基乙酰丙酸对黄瓜幼苗抗冷性的影响[J].西北农业学报,2007,16(4):166-169.
• [11]FENG Zhi-wei,GUO Shang,MAO Li-ping,ZHAO Cheng-feng.Effect of ALA treatment to quality and yield in spinach[J].J ShanxiAgri Sci,2009,37(5):42-43.冯志威,郭尚,毛丽萍,赵成风.ALA处理对菠菜品质和产量的影响[J].山西农业科学,2009,37(5):42-43.
• [12]QI Xiang-ling,ZOU Zhi-rong,YANG Rui.Mitigative effect of 5-aminolevulinic acid on lettuce under NaCl stress[J].Acta Bot Boreal-Occidental Sin,2008,17(2):202-206.祁向玲,邹志荣,杨蕊.外源ALA对NaCl胁迫下生菜植株的缓解效应[J].西北农业学报,2008,17(2):202-206.
• [13]LIU Wei-qin,KANG Lang,WANG Liang-ju.Effects on strawberry photosynthesis and relations to anti-oxidant enzymes of ALA[J].Acta Bot Boreal-Occidental Sin,2006,26(1):57-62.刘卫琴,康琅,汪良驹.ALA对草莓光合作用的影响及其与抗氧化酶的关系[J].西北植物学报,2006,26(1):57-62.
• [14]SHEN Ming,DUAN Chun-hui,ZHANG Zhi-ping,CHENG Yun,WANG Liang-ju,LI Bai-jian.Effects of exogenous ALA on thinning and fruit quality in’Hosui’pear(Pyrus pyrifolia)[J].Acta Hort Sin,2011,38(8):1515-1522.申明,段春慧,张治平,程云,汪良驹,李百健.外源ALA处理对‘丰水’梨疏花与果实品质的影响[J].园艺学报,2011,38(8):1515-1522.
• [15]WANG Liang-ju,WANG Zhong-hua,LI Zhi-qiang,LIU Hui,LIU Wei-qin,CHEN Zong-yuan,YAN Pu,SUN Dun-qian.Effect of 5-aminolevulinic acid on enhancing apple fruit coloration[J].J Fruit Sci,2004,21(6):512-515.汪良驹,王中华,李志强,刘晖,刘卫琴,陈宗元,颜璞,孙敦乾.5-氨基乙酰丙酸促进苹果果实着色的效应[J].果树学报,2004,21(6):512-515.
• [16]WANG Zhong-hua,TANG Guo-hui,LI Zhi-qiang,WANG Liangju.Promotion of 5-aminolevulinic acid and genistein on anthocyanin accumulation in apples[J].Acta Hort Sin,2006,33(5):1055-1058.王中华,汤国辉,李志强,汪良驹.5-氨基乙酰丙酸和金雀异黄素促进苹果果皮花青素形成的效应[J].园艺学报,2006,33(5):1055-1058.
• [17]XIE L,WANG Z H,CHENG X H,GAO J J,ZHANG Z P,WANG L J.5-Aminolevulinic acid promotes anthocyanin accumulation in Fuji apples[J].Plant Growth Regulation,2013,69(3):295-303.
• [18]APPENROTH K J,STCKEL J,SRIVASTAVA A,STRASSER R J.Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements[J].Environ Poll,2001,115:49-64.
• [19]KRGER G H J,TSIMILLI-MICHAEL M,STRASSER R J.Light stress provokes plastic and elastic modifications in structure and function of photosystem II in camellia leaves[J].Physiol Plant,1997,101:265-277.
• [20]THACH L B,SHAPCOTT A,SCHMIDT S,CRITCHLEY C.The OJIP fast fluorescence rise characterizes Graptophyllum species and their stress responses[J].Photosynth Res,2007,94:423-436.
• [21]LI Peng-min,GAO Hui-yuan,STRASSER R J.Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study[J].Acta Photophysiol Sin,2005,31(6):559-566.李鹏民,高辉远,Strasser R J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.
• [22]HONDA C,KOTODA N,WADA M,KONDO S,KOBAYASHI S,SOEJIMA J,ZHANG Z,TSUDA T,MORIGUCHI T.Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin[J].Plant Physiol Biochem,2002,40:955-962.
• [23]LI He-sheng.Techniques of plant physiological biochemical experiment[M].Beijing:Higher Education Press,2003:195-197.李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2003:195-197.
• [24]BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding[J].Anal Biochem,1976,72:248-254.
• [25]WANG S Y,BUNCE J A,MAAS J L.Elevated carbon dioxide increases contents of antioxidant compounds in field-grown strawberries[J].J Agri Food Chem,2003,51(15):4315-4320.
• [26]LI Can-ying,CHANG Yong-yi,SHANG Jia-yin,GE Yong-hong.Effect of bagging on pigment of berry skin and fruit quality in Red Globe grape[J].Sino-Overseas Grapevine Wine,2006(2):9-12.李灿婴,常永义,商佳胤,葛永红.套袋对红地球葡萄果皮色素和果实品质的影响[J].中外葡萄与葡萄酒,2006(2):9-12.
• [27]ZHANG Zhi-ping.Changes of physiological characteristics of the obtained trangenic YHem1 plants with ability to over-produce 5-aminolevulinic acid[D].Nanjing:Nanjing Agriculture University,2010.张治平.四种转ALA合酶基因(YHem1)植物的获得及其生理特性变化的研究[D].南京:南京农业大学,2010.
• [28]ESPINAS N A,KOBAYASHI K,TAKAHASHI S,MOCHIZUKI N,MASUDA T.Evaluation of unbound free heme in plant cells by differential acetone extraction[J].Plant Cell Physiol,2012,53(7):1344-1354.
• [29]PELLETIER H,KRAUT J.Crystal structure of a complex between electron transfer partners,cytochrome c peroxidase and cytochromec[J].Sci,1992,258(5089):1748-1755.
• [30]SHEN M,ZHANG Z P,WANG L J.Effect of 5-aminolevulinic acid(ALA)on leaf diurnal photosynthetic characteristics and antioxidant activity in pear(Pyrus pyrifolia Nakai)[M]//In:Artificial Photosynthesis.NAJAFPOUR M M.Published by InTech,2011:239-265.
• [31]TANAKA Y,TANAKA A,TSUJI H.Effect of 5-aminolevulinic acid on the accumulation of chlorophyll b and apportions of the light harvesting chlorophyll a/b-protein complex of photosystem II[J].Plant Cell Physiol,1993,34:465-472.
• [32]ZHANG Z P,YAO Q H,WANG L J.Expression of yeast Hem1 controlled by Arabidopsis HemA1 promoter enhances leaf photosynthesis in transgenic tobacco[J].Mol Biol Rep,2011,38:4369-4379.
• [33]LIN Xiao-hui,ZHANG Zhi-ping,HAN Li-xiao,WU Zhen,WANG Liang-ju.Effect of transformed recombinant ALA synthase gene(YHem1)on leaf photosynthetic energy conversion in strawberry[J].J Fruit Sci,2011,28(6):1038-1044.林晓慧,张治平,韩丽晓,吴震,汪良驹.重组ALA合酶基因(YHem1)转化草莓提高叶片光合能量转化能力的效应[J].果树学报,2011,28(6):1038-1044.