陈聃;时浩杰;吴慧明;徐志宏;张传清;

• 1:浙江农林大学农业与食品科学学院植物保护系

摘要(Abstract)：

【目的】了解浙江地区葡萄炭疽病菌(Colletotrichum gloeosporioides)对苯并咪唑类杀菌剂和甾醇脱甲基抑制剂(DMI)类杀菌剂的抗药性现状,为其防治提供依据和指导。【方法】采用区分剂量法测定了浙江省临安市、诸暨市和金华市的3个葡萄重要产区的108个葡萄炭疽病菌株对甲基硫菌灵和戊唑醇的抗药性,并对甲基硫菌灵敏感和抗药性菌株中的β-tubulin基因进行了扩增、测序和比较。【结果】所采集菌株群体对甲基硫菌灵的抗药性频率为37.04%,且均为高水平抗药性菌株(Ben HR),Ben HR对多菌灵和乙霉威表现双重抗药性,其β-tubulin基因的第200位密码子从TTC突变为TAC,导致第200位氨基酸从苯丙氨酸(Phe)突变为络氨酸(Tyr)。所采集菌株群体共检测到33个(30.56%)对戊唑醇表现低水平抗药性的菌株。【结论】浙江地区葡萄炭疽病菌对甲基硫菌灵、乙霉威产生了严重的抗药性,对DMIs类杀菌剂戊唑醇的抗药性发展迅速。

关键词(KeyWords)： 葡萄炭疽病菌;甲基硫菌灵;乙霉威;戊唑醇;抗药性;β-tubulin基因

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项(编号:201303023);; 浙江省三农六方“避雨栽培葡萄主要病虫防控技术研究及应用”;; 浙江省公益技术应用研究项目(2010C32083)

作者(Author): 陈聃;时浩杰;吴慧明;徐志宏;张传清;

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