秦栋;张椿浩;刘庆帅;薛晓晓;霍俊伟;员盎然;

• 1:东北农业大学园艺园林学院·农业部东北地区园艺作物生物学与种质创新重点实验室

摘要(Abstract)：

【目的】分析黑穗醋栗二次萌芽过程中超微结构特征及变化规律,为全面揭示黑穗醋栗二次萌芽的内在机制提供科学依据。【方法】以黑穗醋栗易二次萌芽品种‘亚德’和不易二次萌芽品种‘巴基拉’为试材,以清水为对照,用30mg·L^(-1)GA_3和50 mg·L(-1)GA_3和50 mg·L^(-1)ABA处理,用透射电镜观察芽内细胞超微结构。【结果】GA_3处理促进2品种二次萌芽加快,使细胞内质壁分离现象消失,胞间连丝、内质网提前分化,淀粉粒加速分解,线粒体更加活跃,分散的小液泡最终形成中心大液泡,且液泡中电子致密物质分解;ABA处理抑制2品种的二次萌芽,使细胞保持质壁分离状态,抑制胞间连丝出现,线粒体、内质网、淀粉粒分化均晚于同时期GA_3处理,ABA抑制了‘巴基拉’液泡中电子致密物质分解。【结论】黑穗醋栗二次萌芽本质是细胞破除休眠的过程。外源GA_3处理加速黑穗醋栗解除休眠,而外源ABA则是促进休眠,2者作用相反。GA_3和ABA影响黑穗醋栗二次萌芽最主要的差异在于细胞内是否存在质壁分离、胞间连丝是否分化和电子致密物质是否分解。细胞超微结构的变化反应了二次萌芽的进程。

关键词(KeyWords)： 黑穗醋栗;二次萌芽;赤霉素(GA_3);脱落酸(ABA);超微结构

Abstract:

Keywords:

基金项目(Foundation): 东北农业大学“青年才俊”(16QC07);; 国家自然科学基金青年科学基金(31201584)

作者(Authors): 秦栋;张椿浩;刘庆帅;薛晓晓;霍俊伟;员盎然;

DOI: 10.13925/j.cnki.gsxb.20160449

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