刘菲菲;李赤;刘勇勤;于莉;

• 1:广东海洋大学农学院

摘要(Abstract)：

以健康香蕉植株和感染香蕉枯萎病菌Fusarium oxysporum f.sp.cubense(FOC)后的香蕉植株的根茎叶组织为材料,提取样品中的总DNA,扩增细菌的16S rDNA的V3可变区,对扩增产物进行DGGE电泳分析,并对其中18条优势条带进行切胶回收、克隆测序和系统发育分析。结果表明,香蕉健株与病株各组织中所含内生细菌的种群丰富度为根部>假茎>叶片;感病植株组织内生细菌种类比健康植株丰富;BLAST结果为大部分克隆序列与已知细菌16SrDNA基因序列的同源性较高(94%~100%),分别归属于蓝细菌门(Cyanobacteria)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteria)、变形杆菌门(Proteobacterium)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和绿菌门(Chlorobi)7大类群;其中一个序列同源性较低(91%),可能代表新的分类单元。

关键词(KeyWords)： 香蕉;内生细菌;DGGE;群落结构;多样性

Abstract:

Keywords:

基金项目(Foundation): 湛江市科技局项目:抗枯萎病香蕉品种的引进与应用

作者(Author): 刘菲菲;李赤;刘勇勤;于莉;

Email:

DOI:

参考文献(References)：

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