吴元立,易干军,周碧容,曾继吾

• 1:广东省农业科学院果树研究所
• 2:广东省农业科学院果树研究所
• 3:广东省农业科学院果树研究所
• 4:广东省农业科学院果树研究所 广东 广州 510640

摘要(Abstract)：

目前香蕉种质的离体保存主要有缓慢生长保存和超低温保存两种方法。综合有关文献综述和讨论了香蕉种质离体保存过程涉及的遗传多样性、遗传稳定性和完整性、适应等遗传学问题。在开展香蕉种质离体保存的过程中,对离体保存所带来的遗传学方面的影响加以重视并设法减少其影响十分重要:(1)香蕉的生物学特性决定了香蕉种质的离体保存主要以始于吸芽茎尖的培养物作为保存材料;为减少取样环节样本容量较小带来的影响,建议保存数量较大的种质份数。(2)离体保存尤其是缓慢生长保存会引起香蕉种质的遗传不稳定性,因此有必要进行定期的检测。目前香蕉种质遗传稳定性的检测方法主要有形态鉴别、超微结构的观察、同工酶分析和分子标记等。(3)与缓慢生长保存相比较,超低温保存除了在保存过程中香蕉种质遗传稳定性较高,在实现对香蕉种质的长期保存方面也具有明显的优越性。

关键词(KeyWords)： 香蕉;种质;离体保存;遗传多样性;遗传稳定性和完整性;适应

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 吴元立,易干军,周碧容,曾继吾

DOI: 10.13925/j.cnki.gsxb.2004.04.020

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