尚霄丽,谭彬,李继东,张建鹏,冯建灿

• 1:河南农业大学
• 2:濮阳职业技术学院

摘要(Abstract)：

【目的】为确定中华猕猴桃遗传转化中适宜的抗生素种类和浓度,【方法】以中华猕猴桃‘伏牛95-2’叶片为外植体,研究了抗生素对外植体再生的影响。【结果】抗生素敏感性测定发现,当卡那霉素质量浓度为20 mg.L-1时,愈伤组织及不定芽分化率均为0,因此,20 mg.L-1卡那霉素为适宜的筛选浓度;头孢霉素和羧苄青霉素对中华猕猴桃‘伏牛95-2’叶片分化均有抑制作用,但头孢霉素对叶片分化的抑制作用比羧苄青霉素小,结合抑菌试验,将浓度400 mg.L-1头孢霉素确定为适宜于中华猕猴桃‘伏牛95-2’叶片遗传转化的抑菌抗生素。【结论】卡那霉素20 mg.L-1和头孢霉素400 mg.L-1为中华猕猴桃‘伏牛95-2’遗传转化适宜的抗生素及质量浓度。

关键词(KeyWords)： 中华猕猴桃‘伏牛95-2’;抗生素;外植体

Abstract:

Keywords:

基金项目(Foundation): 河南省杰出青年基金项目(084100410021)

作者(Author): 尚霄丽,谭彬,李继东,张建鹏,冯建灿

DOI: 10.13925/j.cnki.gsxb.2012.04.023

参考文献(References)：

• [1]FAN Jun-feng,LI Jia-rui,HAN Yi-fan,LI Ling,PENG Xue-xian.Studies on transformation of mtID/gutD salt-resistant gene to ki-wifruit(Qin mei)[J].Journal of Northwest Sci-Tech University of A-griculture and Forestry:Natural Science Edition,2002,30(3):53-58.樊军锋,李嘉瑞,韩一凡,李玲,彭学贤.mtID/gutD双价耐盐基因转化秦美猕猴桃的研究[J].西北农林科技大学学报:自然科学版,2002,30(3):53-58.
• [2]WANG Shun-cai.Study on transformation of Kiwifruit(ActinidiaDeliciosa)with Mn-SOD Gene[D].Yangling:Norhwest A&F Uni-versity,2004:55-56.王顺才.Mn-SOD基因转化猕猴桃(Actinidia deliciose)的研究[D].杨凌:西北农林科技大学,2004:55-56.
• [3]BI Jing-hua,GAO Yue,LIU Yong-li,YE Qing-fu.Studies on an-tibiotics sensitivity and genetic transformation of Actinidia latifolia[J].Journal of Nuclear Agricultural Sciences,2006,20(4):287-291.毕静华,高月,刘永立,叶庆富.阔叶猕猴桃抗生素敏感性及遗传转化的研究[J].核农学报,2006,20(4):287-291.
• [4]HUANG Ping,SHEN Xiao-shan,MA Chao-hong.Effect of antibi-otics on callus induction and differentiation in Actinidia chinensis[J].Guizhou Agricultural Sciences,2002,30(6):6-7.黄萍,沈孝善,马朝红.抗生素对猕猴桃叶柄愈伤组织诱导及分化的影响[J].贵州农业科学,2002,30(6):6-7.
• [5]HU Gui-bing,CHEN Da-cheng,ZHENG Qi-fa,HUANG Zi-ran.Effect of antibiotics on growth of stem segments and callus of Ber(Ziziphus mauritiana Lam.)[J].Journal of South China AgriculturalUniversity,2001,22(2):21-23.胡桂兵,陈大成,郑启发,黄自然.抗生素对台湾青枣茎段和愈伤组织生长的影响[J].华南农业大学学报,2001,22(2):21-23.
• [6]LIU Shu-fang,TANG Hao-ru,WANG Mei,LIU Xiang-lin.Effectsof several antibiotics on callus induction and shoot regeneration fromin vitro leaves of pears[J].Journal of Fruit Science,2005,22(5):18-21.刘淑芳,汤浩茹,王梅,刘祥林.几种抗生素对梨叶片愈伤组织和不定梢诱导的影响[J].果树学报,2005,22(5):18-21.
• [7]LIU Jing,ZHANG Yuan-hu,LIU Qing-zhong,GAO Yu-jiao.Ef-fects of antibiotics on leaf regeneration of apple cultivar White winterpearmain[J].Shangdong Agricultural Sciences,2009,7:53-55.刘静,张元湖,刘庆忠,高玉娇.抗生素对青香蕉苹果叶片再生的影响[J].山东农业科学,2009,7:53-55.
• [8]GAO Xi-ye,LI Xiao-yan,LI Ming,ZHANG Xiang-wen.Effects ofseveral antibiotics on adventitious bud and root regeneration from invitro leaves of prunus cerasus[J].Journal of Inner Mongolia Agricul-tural University:Natural Science Edition,2007,28(3):242.高喜叶,李晓燕,李明,张相文.几种抗生素对酸樱桃离体培养产生不定芽与再生根影响[J].内蒙古农业大学学报:自然科学版,2007,28(3):242.
• [9]WANG Mei,TANG Hao-ru,LIU Shu-fang,LIU Xiang-lin,LIJing.Inhibition of four kinds of antibiotics bacteria in‘Zoji’straw-berry and their effects on the growth of its in vitro shoots[J].Journalof Sichuan Agricultural University,2005,23(2):189-191.王梅,汤浩茹,刘淑芳,刘祥林,李靖.4种抗生素对草莓‘章姬’内生菌的抑制及对试管苗生长的影响[J].四川农业大学学报,2005,23(2):189-191.
• [10]WANG Yan-li,HE Zhu,SHI Xiao-xin,DU Guo-qiang.The selec-tion of antibiotics and their concentration during gene transformationvia leaf disc regeneration system in grapes[J].Chinese AgriculturalScience Bulletin,2009,25(21):59-63.王彦立,贺柱,师校欣,杜国强.葡萄叶盘法基因转化中抗生素种类和浓度的筛选[J].中国农学通报,2009,25(21):59-63.
• [11]ZHENG Jin,KANG Wei,WANG Hui,LIU Kai-yu,HONG Hua-zhu,PENG Jian-xin.Effects of kanamycin on the growth and deter-mination of popla and blacust[J].Journal of Nuclear Agricultural Sci-ence,2006(2):37-38.郑进,康薇,王慧,刘凯于,洪华珠,彭建新.卡那霉素对杨树和刺槐外植体生长的影响[J].核农学报,2006(2):37-38.
• [12]NIU Zheng-tian,ZHANG Xiu-hai,LUO Xiao-fang.Determinationfor the sensitivity of Black Locust to three antibiotics[J].Plant Physi-ology Communications,2004(1):100-101.牛正田,张秀海,罗晓芳.刺槐对3种抗生素敏感性的测定[J].植物生理学通讯,2004(1):100-101.
• [13]LIN J J,NACYRA A G,JONATHON K.Plant hormone effect of an-tibioticson transformation efficient of plant tissue by Agrobacteriumtumefaciens cells[J].Plant Science,1995,109:171-177.
• [14]LI M,HUANG Z,HAN L,ZHAO G,LI Y.Gene transfer strategyfor kiwifruit to obtain pure transgenic plant through inducing adven-titious roots in leaf explants with Agrobacterium tumefaciens[J].ActaHorticulturae(ISHS),2003,610:495-499.