关鹏;曾斌;李疆;罗淑萍;王建友;李伟阳;田嘉;李鹏;

• 1:新疆农业大学农学院
• 2:新疆农业大学林学与园艺学院·新疆农业大学果树学新疆特色果树研究中心
• 3:中国林业科学院新疆分院

摘要(Abstract)：

【目的】克隆新疆野扁桃(Amygdalus ledebouriana Schlecht.)自交不亲和性花柱特异性决定因子编码基因SRNase全长序列,为自交不亲和性的分子调控奠定基础。【方法】以新疆野扁桃花柱为试材,利用RT-PCR和RACE技术克隆S-RNase基因全长,采用BLAST和ORF Finder对核酸序列进行分析,利用CDD、Prot Param、Tmpred、Signal P、Target P、SOPMA、DANMAN、MEGA6和Prot Fun对推导的氨基酸序列进行分析。【结果】克隆到Pt S16-RNase基因和Pt S17-RNase基因,2者均属于RNase T2基因家族,与其他多种植物的S-RNase基因的序列相似度为83%⁹8%,序列均具有S-RNas蛋白典型结构。Pt S16-RNase基因ORF长690 bp,编码229个氨基酸,Pt S17-RNase基因ORF长678 bp,编码225个氨基酸。预测2个S-RNase蛋白均为亲水性、不稳定的分泌蛋白,二级结构均以α-螺旋、延伸链和无规卷曲为主,在蔷薇科李属植物中具有较高的系统进化一致性,可能的主要功能为水解酶和激素。【结论】获得2个新疆野扁桃自交不亲和性花柱特异性决定因子编码基因S-RNase全长序列。

关键词(KeyWords)： 野扁桃;自交不亲和性;S-RNase基因;生物信息学;RACE

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31260465);; 国家林业公益性行业科研专项(201304701-1,201304701-5);; 新疆维吾尔自治区科技计划项目(201130102-1-5);; 新疆维吾尔自治区果树学重点学科(201007)

作者(Authors): 关鹏;曾斌;李疆;罗淑萍;王建友;李伟阳;田嘉;李鹏;

DOI: 10.13925/j.cnki.gsxb.20150520

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