杜梨2个MYB转录因子基因的克隆及序列分析Cloning and sequence analysis of two MYB transcription factors in Pyrus betulifolia
冉昆;王少敏;董放;魏树伟;张勇;王宏伟;
摘要(Abstract)：
【目的】克隆杜梨(Pyrus betulifolia Bunge)MYB家族的2个基因(Pb3RMYB和Pb4RMYB),分析其序列特征,为研究其调控的分子机制奠定基础。【方法】利用RT-PCR和RACE技术克隆杜梨Pb3RMYB和Pb4RMYB的c DNA序列全长;利用BLAST和ORF Finder对核酸序列进行分析,利用CDD、Prot Param、TMHMM、Signal P、Plant-m Ploc、SPOMA、DANMAN、MEGA6等软件对氨基酸序列进行分析。【结果】克隆得到Pb3RMYB和Pb4RMYB基因全长,Gen Bank登录号分别为KX272614和KX272615。2者均含有MYB保守结构域,二级结构均以无规则卷曲、α-螺旋、延伸链和β-折叠为主,亚细胞定位分析表明2者均定位于细胞核中。其中,Pb3RMYB属于3R-MYB亚家族,全长2 355 bp,ORF长1 710bp,编码569个氨基酸,蛋白分子质量约为62 077.3 Da,等电点为7.08;BLAST分析表明Pb3RMYB与已报道的其他物种3RMYB具有较高的相似度。Pb4RMYB属于4R-MYB亚家族,全长3 625 bp,ORF长2 913 bp,编码970个氨基酸,蛋白分子质量约为109 600.6 Da,等电点为7.41。【结论】获得了2个杜梨MYB基因Pb3RMYB和Pb4RMYB,分别属于3RMYB和4R-MYB亚家族。
关键词(KeyWords)： 杜梨;MYB转录因子;基因克隆;序列分析
基金项目(Foundation): 山东省自然科学基金(ZR2015YL075);; 山东省农业科学院青年科研基金(2015YQN41);; 山东省良种工程项目(梨优质矮化、多抗功能基因挖掘与种质创新利用2014);; 现代农业产业技术体系建设专项(CARS-29-31)
作者(Author): 冉昆;王少敏;董放;魏树伟;张勇;王宏伟;
Email:
DOI: 10.13925/j.cnki.gsxb.2016.S.06
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