白丹凤;李志;齐秀娟;陈锦永;顾红;黄武权;任建杰;钟云鹏;方金豹;

• 1:中国农业科学院郑州果树研究所·中国农业科学院果树生长发育与品质控制重点开放实验室
• 2:浙江省绍兴市上虞区农业技术推广中心

摘要(Abstract)：

【目的】研究淹水胁迫下4种基因型猕猴桃的形态变化及生理响应,以筛选耐涝种质资源,并初步分析猕猴桃耐涝的生理机制。【方法】采用"双套盆"法模拟淹水胁迫,对大籽猕猴桃'KR2’、对萼猕猴桃'KR5’、美味猕猴桃'15-13’及软枣猕猴桃'永丰一号’的两年生组培盆栽苗进行淹水处理,通过统计涝害指数观察淹水对此4种猕猴桃属植物形态的影响,同时通过测定根系活力、叶片光合能力及根系低氧伤害相关指标研究淹水对猕猴桃属4种植物生理代谢的影响,并通过隶属函数法综合评价4份材料的耐涝性。【结果】随着淹水胁迫时间的延长,4种猕猴桃属植物的涝害指数均呈现逐渐上升的趋势,根系活力逐渐下降,光合相关指标呈现先上升后下降的趋势,超氧阴离子产生速率、过氧化氢及丙二醛含量总体呈现逐渐上升的趋势。但在整个淹水胁迫期间',KR2'’KR5’仍能保持较高的根系活力与光合能力,且受到的低氧伤害较小。隶属函数法综合评价结果表明,4种猕猴桃属植物的耐涝性顺序为':KR2’>'KR5’>'15-13’>'永丰一号’。【结论】4种猕猴桃中',KR2’和'KR5’的耐涝性较强',15-13’和'永丰一号’属不耐涝猕猴桃。耐涝猕猴桃在涝害胁迫下可以缓解低氧伤害,维持自身的根系活力,保证地上部的健康生长,进而保证地上部叶片高水平的光合能力,从而表现出较强的耐涝性。

关键词(KeyWords)： 猕猴桃;基因型;耐涝性;生理机制

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金(31801846);; 中国农业科学院科技创新工程(CAAS-ASTIP-2017-ZFRI);; 河南省现代农业产业技术体系(S2014-11)

作者(Author): 白丹凤;李志;齐秀娟;陈锦永;顾红;黄武权;任建杰;钟云鹏;方金豹;

Email:

DOI:

参考文献(References)：

• [1]张星.淹水胁迫对不同土壤类型理化特性及玉米养分吸收分配的影响[D].郑州:河南农业大学,2017.ZHANG Xing. Effects of waterlogging stress on physicochemi-cal properties and maize nutrient absorption and distribution indifferent soil types[D]. Zhengzhou:Henan Agricultural Universi-ty,2017.
• [2] JACKSON M B,ARMSTRONG W. Formation of aerenchymaand the processes of plant ventilation in relation to soil floodingand submergence[J]. Plant Biology,1999,1(3):274-287.
• [3]张建华.水稻生长过程对涝害的响复模型研究[D].福州:福建农林大学,2013.ZHANG Jianhua. A study on the response and recovery of mod-els of rice growth processes to waterlogging disaster[D]. Fu-zhou:Fujian Agriculture and Forestry University,2013.
• [4]向镜,陈惠哲,张玉屏,张义凯,朱德峰.淹涝条件下水温对水稻幼苗形态和生理的影响[J].中国水稻科学,2016,30(5):525-531.XIANG Jing,CHEN Huizhe,ZHANG Yuping,ZHANG Yikai,ZHU Defeng. Morphological and physiological response of riceseedlings to water temperature under complete submergence[J].China Journal Rice Science,2016,30(5):525-531.
• [5]尹冬梅.菊花近缘种属植物涝性评价及耐涝机理研究[D].南京:南京农业大学,2011.YIN Dongmei. Evaluation on waterlogging tolerance and itsmechanisms in chrysanthemum morifolium and its related spe-cies[D]. Nanjing:Nanjing Agricultural University,2011.
• [6]徐子棋,饶良懿,朱金兆,赵洋.水淹胁迫下饲料桑苗的生长及光合响应[J].农业工程学报,2015,31(22):105-114.XU Ziqi,RAO Liangyi,ZHU Jinzhao,ZHAO Yang. Growth andphotosynthesis characteristics of mulberry under flooding stress[J]. Transactions of the Chinese Society of Agricultural Engi-neering,2015,31(22):105-114.
• [7]齐琳,马娜,吴雯雯,安玉艳,徐君成,秦祥宏,汪良驹.无花果品种幼苗淹水胁迫的生理响应与耐涝性评估[J].园艺学报,2015,42(7):1273–1284.QI Lin,MA Na,WU Wenwen,AN Yuyan,XU Juncheng,QINXianghong,WANG Liangju. Physiological responses and toler-ance evaluation of fig cultivars to waterlogging[J]. Acta Horti-culturae Sinica,2015,42(7):1273–1284.
• [8]王阳,汪李平,杨静,王润东.甜瓜幼苗对淹水胁迫响应的形态差异及根系生理指标的比较[J].现代园艺,2017,1(2):7-8.WANG Yang,WANG Liping,YANG Jing,WANG Rundong.Morphological differences in response of muskmelon seedlingsto flooding stress and comparison of Root physiological Indexes[J]. Xiandai Horticulture,2017,1(2):7-8.
• [9]李艳,付艳东,马艳春,杜远鹏,翟衡.淹水对不同葡萄砧木生长及光合特性的影响[J].中国农业科学,2013,46(5):995-1004.LI Yan,FU Yandong,MA Yanchun,DU Yuanpeng,ZHAI Heng.Effects of waterlogging on characteristics of growth and photo-synthesis in different grape rootstocks[J]. Scientia AgriculturaSinica,2013,46(5):995-1004.
• [10] NISHUICHI S,YAMAUCHI T,TAKAHASHI H,KOTULA L,NAKAZONO M. Mechanisms for coping with submergenceand waterlogging in rice[J]. Rice,2012,5(1):1-14.
• [11] ABIKO T,KOTULA L,SHIONO K,MALIK A I,COLMER TD,NAKAZONO M. Enhanced formationof aerenchyma and in-duction of a barrier to radial oxygen loss in adventitious roots ofZea nicaraguensis contribute to its waterlogging tolerance ascompared with maize(Zea mays ssp. mays)[J]. Plant Cell&En-vironment,2012,35(9):1618-1630.
• [12] YAVAS I,UNAY A,AVDIN M. The waterlogging tolerance ofwheat varieties in western of Turkey[J]. The Scientific WorldJournal,2012(12):1-7.
• [13]黄宏文.猕猴桃研究进展Ⅱ[M].北京:科学出版社,2003.HUANG Hongwen. Research progress of kiwifruitⅡ[M]. Bei-jing:Science Press,2003.
• [14]王莉,王圣梅,黄宏文.猕猴桃属种间嫁接亲和性试验研究及抗根结线虫砧木的初步筛选[J].武汉植物学研究,2001,19(1):47-51.WANG Li,WANG Shengmei,HUANG Hongwen. Graft com-patibility among Actinidia species and screening rootstocks resis-tant to root-knot nematodes[J]. Journal of Wuhan Botanical Re-search,2001,19(1):47-51.
• [15] SHARMA S,SHARMA N. Effect of rootstocks on leaf water po-tential,water relations,antioxidant activities and drought toler-ant in flemish beauty pear under water stress conditions[J]. Indi-an Journal of Plant Physiology,2008,13(3):266-271.
• [16]李艳,杜远鹏,付艳东,翟衡.不同砧木嫁接的赤霞珠葡萄对淹水的生理响应[J].园艺学报,2013,40(11):2105-2114.LI Yan,DU Yuanpeng,FU Yandong,ZHAI Heng. Physiologicalresponses of waterlogging on different rootstock combinationsof Cabernet Sauvignon grape[J]. Acta Horticulturae Sinica,2013,40(11):2105-2114.
• [17]郭洪,赵密珍,周建涛.若干桃砧木的抗涝性[J].中国南方果树,1999,28(2):47.GUO Hong,ZHAO Mizhen,ZHOU Jiantao. Waterlogging toler-ance of peach rootstocks[J]. South China Fruits,1999,28(2):47.
• [18]高俊凤.植物生理学试验技术[M].西安:世界图书出版社,2000:159-198.GAO Junfeng. Plant physiology test technique[M]. Xi’an:World Book Publishing Press,2000:159-198.
• [19]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990,31(6):55-57.WANG Aiguo,LUO Guanghua. Quantitative relation betweenthe reaction of hydroxylamine and superoxide anion radicals inplants[J]. Plant Physiology Communication,1990,31(6):55-57.
• [20]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-194.LI Hesheng. Principles and techniques of plant physiology andbiochemistry experiments[M]. Beijing:Higher Education Press,2000:164-194.
• [21]曾宪海,安锋,蔡明道,陈俊明,林位夫.高渗胁迫后橡胶树萌发籽苗抗旱性主成分及隶属函数析[J].中国农学通报,2010,26(1):260-264.ZENG Xianhai,AN Feng,CAI Mingdao,CHEN Junming,LINWeifu. Analysis of the principal component and the subordinatefunction on rubber seedling drought resistance under hypertonicsolution stresses[J]. Chinese Agricultural Science Bulletin,2010,26(1):260-264.
• [22]郑佳秋,郭军,梅燚,吴永成,祖艳侠,王薇薇.辣椒幼苗形态及生理特性对涝害胁迫的响应[J].西南农业学报,2016,29(3):536-540.ZHENG Jiaqiu,GUO Jun,MEI Yi,WU Yongcheng,ZU Yanxia,WANG Weiwei. Response of morphology and physiologicalcharacteristics of hot pepper seedling to waterlogging sterss[J].Southwest China Journal of Agricultural Sciences,2016,29(3):536-540.
• [23]郁万文,蔡金峰,高长忠.不同桃砧类型对淹水胁迫的生理响应及耐涝性评价[J].中国果树,2016(3):1-6.YU Wanwen,CAI Jinfeng,GAO Changzhong. Physiological re-sponse and waterlogging tolerance of different peach rootstocksto flooding stress[J]. China Fruits,2016(3):1-6.
• [24]张琛.三个类型猕猴桃品种实生苗对淹水胁迫的生理反应及其耐涝性比较[D].杭州:浙江师范大学,2013.ZHANG Chen. Tolerance compasision and physiology responseof three kiwifruit cultivars to waterlogging[D]. Hangzhou:Zheji-ang Normal University,2013.
• [25] AHMED S,NAWATA E,HOSOKAWA M,DOMAE Y,SAKU-RATANI T. Alterations in photosynthesis and some antioxidantenzymatic activities of mungbean subjected to waterlogging[J].Plant Science 2002,163(1):117-123.
• [26]刘凤礼.淹水对不同砧木猕猴桃生理生化特性的影响[D].杭州:浙江农林大学,2015.LIU Fengli. Effects of the physiological and biochemical charac-ters of kiwifruit growed on different stocks with waterloggingtreatment[D]. Hangzhou:Zhejiang A&F University,2015.
• [27]张琛,张慧琴,肖金平,马常念,谢鸣.三个品种猕猴桃实生苗的耐涝性比较[J].浙江农业学报,2013,25(5):1007-1012.ZHANG Chen,ZHANG Huiqin,XIAO Jinping,MA Changnian,XIE Ming. Comparison on seedling waterlogging tolerance ofthree cultivars of Actinidia chinensis Planch[J]. Acta Agricul-turae Zhejiangensis,2013,25(5):1007-1012.
• [28]马瑞娟,张斌斌,蔡志翔,沈志军,俞明亮.不同桃砧木品种对淹水的光合响应及其耐涝性评价[J].园艺学报,2013,40(3):409-416.MA Ruijuan,ZHANG Binbin,CAI Zhixiang,SHEN Zhijun,YUMingliang. Evaluation of peach rootstock waterlogging toler-ance based on the responses of the photosynthetic indexes tocontinuous submergence stress[J]. Acta Horticulturae Sinica,2013,40(3):409-416.
• [29]杜秀敏,殷文璇,赵彦修,张慧.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121-125.DU Xiumin,YIN Wenxuan,ZHAO Yanxiu,ZHANG Hui. Pro-duction and scavenging mechanism of reactive oxygen speciesin plants[J]. Chinese Journal of Biotechnology,2001,17(2):121-125.
• [30]陈由强,朱锦懋,叶冰莹.水分胁迫对芒果(Mangifera indicaL.)幼叶细胞活性氧伤害的影响[J].生命科学研究,2000,4(1):60-64.CHEN Youqiang,ZHU Jinmao,YE Bingying. Effects of droughtstress on active oxygen damage and membrane lipid peroxida-tion of leaves in mango(Mangifera indica L.)[J]. Life ScienceResearch,2000,4(1):60-64.
• [31]米银法,马锋旺,马小卫.根际低氧对不同抗性猕猴桃幼苗抗氧化系统的影响[J].中国农业科学,2008,41(12):4328-4335.MI Yinfa,MA Fengwang,MA Xiaowei. Effect of root-zone hy-poxia stress on anti-oxidative system of Chinese gooseberryseedlings with different resistances[J]. Scientia Agricultura Sini-ca,2008,41(12):4328-4335.
• [32]陈杰,高青海,马横宇,曹笑.西瓜砧木苗期耐涝性鉴定及指标选择[J].北方园艺,2018(8):7-13.CHEN Jie,GAO Qinghai,MA Hengyu,CAO Xiao. Identifica-tion for flooding stress tolerance and selection for indexes of wa-termelon root stock at seedling stage[J]. Northern Horticulture,2018(8):7-13.
• [33]陈春芳.四种石楠属苗木对土壤水分胁迫的生理响应[D].南京:南京林业大学,2009.CHEN Chunfang. Physiological responses of four photinia seed-lings to water stress[D]. Nanjing:Nanjing Forestry University,2009.
• [34]熊英,欧阳杰,何永歆,黄乾龙,王静.芽期耐低温淹水的水稻种质的评价与筛选[J].杂交水稻,2015,30(4):54-58.XIONG Ying,OUYANG Jie,HE Yongxin,HUANG Qianlong,WANG Jing. Evaluation and screening of rice genetic resourceswith tolerance to low temperature and submergence in germinat-ing stage[J]. Hybrid Rice,2015,30(4):54-58.