支玉玺;张剑侠;王跃进;

• 1:农业部西北园艺植物种质利用重点开放实验室
• 2:陕西省农业分子生物学实验室
• 3:西北农林科技大学园艺学院

摘要(Abstract)：

为了提供葡萄转抗病基因功能验证及遗传转化研究的再生技术体系,以中国野生华东葡萄(V.pseudoretic-ulata)感白粉病株系广西-2的花药为试材,进行了体细胞胚诱导与再生体系的建立,并探讨了次生胚的诱导与畸形萌发胚状体正常成苗的方法。结果表明,广西-2花药在B5+6-BA4.0mg·L-1+2,4-D0.7mg·L-1+CH1.0g·L-1+PVP1.0g·L-1+蔗糖30.0g·L-1培养基上继代3个月,可诱导胚性愈伤组织产生;B5+6-BA4.0mg·L-1+CH1.0g·L-1+PVP1.0g·L-1+蔗糖15.0g·L-1培养基有利于胚状体形成;胚状体在1/2B5+CH1.0g·L-1+PVP1.0g·L-1+蔗糖15.0g·L-1的液体培养基比相同成分固体培养基中早萌发14d左右;已萌发胚状体在WPM+IBA0.15mg·L-1+AC0.5g·L-1+蔗糖15.0g·L-1培养基上可以生根成苗;MS+6-BA1.5mg·L-1+IBA0.2mg·L-1+蔗糖30.0g·L-1培养基有利于畸形萌发胚状体正常成苗。发育早期胚状体在B5+6-BA1.0mg·L-1+2,4-D0.5mg·L-1+CH1.0g·L-1+PVP1.0g·L-1+蔗糖30.0g·L-1培养基上可形成次生胚。

关键词(KeyWords)： 中国野生葡萄;华东葡萄;花药;胚状体诱导;再生植株

Abstract:

Keywords:

基金项目(Foundation): 国家863计划项目(2007AA10Z182);; 国家自然科学基金(30771493);; 农业部948项目(2005-Z40);; 现代农业产业技术体系专项资金资助

作者(Author): 支玉玺;张剑侠;王跃进;

Email:

DOI: 10.13925/j.cnki.gsxb.2010.01.006

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