中国野生葡萄抗霜霉病基因RAPD标记的筛选Screening the RAPD markers linked to the gene resistant to downy mildew in Chinese wild species of Vitis
张艳艳;张剑侠;王跃进;
摘要(Abstract):
以抗病的中国野生华东葡萄(Vitis pseudoreticulata)白河-35-1(Baihe-35-1)与感病的欧洲葡萄佳利酿(Carignane)杂交F2代为试材,从520条随机引物中筛选出21条可以扩增多态性DNA片断的随机引物,从中获得了6个抗霜霉病基因的RAPD标记,对这些标记回收、克隆、测序后,将得到的6个标记分别记录为:S294-369,S202-527,S382-615,S221-677,S416-1224和S390-725。通过分析这6个RAPD标记在F2代植株中的表现,证明这些标记都与霜霉病抗性的基因有连锁关系。这将为葡萄抗霜霉病育种的分子标记辅助选择提供分子依据。
关键词(KeyWords): 中国野生葡萄;霜霉病;抗病基因;RAPD标记
基金项目(Foundation): 国家自然科学基金(No.30571280)
作者(Author): 张艳艳;张剑侠;王跃进;
Email:
DOI:
参考文献(References):
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