邓云,王志伟,孙德玺,芦金生,马双武,朱迎春,刘君璞

• 1:中国农业科学院郑州果树研究所
• 2:四川农业大学园艺学院
• 3:北京市大兴区农业科学研究所

摘要(Abstract)：

【目的】为了研究准确鉴定评价西瓜种质资源抗南方根结线虫侵染能力的方法,筛选抗性材料,【方法】以35份西瓜种质资源为试材,采用盆栽幼苗人工接种方法,测定了南方根结线虫侵染60 d时西瓜幼苗各器官相对生长量及相关抗病指标,并进行了聚类分析和隶属函数分析。【结果】结果表明,南方根结线虫侵染后不同西瓜种质资源幼苗各器官的相对生长量、抗病指标及其变异系数的影响显著不同;采用变异系数最大的根结指数进行聚类分析结果与4个抗病指标综合分析结果存在微小差异,但与5个相对生长量指标以及相对茎鲜质量的聚类分析结果存在较大差异。根据综合指标隶属函数分析,确定了西瓜种质资源抗南方根结线虫的顺序。【结论】采用多指标综合聚类分析与单一指标聚类分析相结合更能准确划分西瓜种质资源抗根结线虫的类群,通过综合鉴定评价,可将供试的35份西瓜种质资源分为高感、感病、低抗3个类群,无中高抗材料,其中抗性最强的是红籽瓜,抗性最弱的是中科6号。

关键词(KeyWords)： 西瓜种质资源;南方根结线虫;相对生长量;抗性指标;聚类分析;隶属函数分析

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项(项目编号:CARS-26-14);; 中国农业科学院基本科研业务费(项目编号:0032012024)

作者(Author): 邓云,王志伟,孙德玺,芦金生,马双武,朱迎春,刘君璞

DOI: 10.13925/j.cnki.gsxb.2012.06.023

参考文献(References)：

• [1]Ministry of Agriculture,PRC.China agricultural statistics report[M].Beijing:China Agriculture Press,2011.中华人民共和国农业部.中国农业统计资料[M].北京:中国农业出版社,2011.
• [2]WANG Zhi-wei,LI Huan-xiu,SUN De-xi,DENG Yun,ZHUYing-chun,LIU Jun-pu.Advance of the research on root-knot ne-matodes in watermelon and melon[J].China Cucurbits and Vegeta-bles,2010(2):31-33.王志伟,李焕秀,孙德玺,邓云,朱迎春,刘君璞.西瓜甜瓜根结线虫病研究进展[J].中国瓜菜,2010(2):31-33.
• [3]ZHAO Bao-xiang,YU Xing-chang,ZHAO Xiao-ling,LIU Zhi-ming.The occurrence of root-knot nematodes on plastic house wa-termelon and comprehensive control measures[J].Agricultural Sci-ence Journal,2001(1):29.赵保祥,于兴昌,赵晓玲,刘之明.大棚西瓜根结线虫病的发生与综合防治[J].农业科技通讯,2001(1):29.
• [4]ZHANG Xue-wei,QIAN Xiao-li,LIU Ji-wei.Evaluation on the re-sistance to root-knot nematode of watermelon germplasm and controlfor it[J].Journal of Fruit Science,1989,8(1):33-38.张学炜,钱笑丽,刘济伟.西瓜种质资源抗根结线虫鉴定及防治研究[J].果树科学,1989,8(1):33-38.
• [5]MONTALVO A E,ESNARD J.Reaction of ten cultivars of water-melon(Citrullus lanatus)to a Puerto Rican population of Meloidog-yne incognita[J].Journal of Nematol,1994,26(suppl):640-643.
• [6]BOYHAN G E,LANGSTON D B,GRANBERRY D M,LEWIS PM,LINTON D O.Resistance to fusarium wilt and root-knot nema-tode in watermelon germplasm[J].Cucurbit Genetics CooperativeReport,2003(26):18-25.
• [7]SHEN DI,LI Xi-xiang,FENG Lan-xiang,WANG Hai-ping,SONG Jiang-ping,YANG Cui-rong,GONG Hui-zhi.Evaluation onresistance of cucurbitaceae gemplasm resources to root-knot nema-tode[J].Journal of Plant Genetic Resources,2007,8(3):340-342.沈镝,李锡香,冯兰香,王海平,宋江萍,杨翠荣,龚会芝.葫芦科蔬菜种质资源对南方根结线虫的抗性评价[J].植物遗传资源学报,2007,8(3):340-342.
• [8]THIES J A,LEVI A.Resistance of watermelon germplasm to thepeanut root-knot nematode[J].Hortscience,2003.38(7):1417-1421.
• [9]THIES J A,LEVI A.Characterization of watermelon(Citrullus lana-tus var.citroides)germplasm for resistance to root-knot nematodes[J].Hortscience,2007,42(7):1530-1533.
• [10]XU Xiao-ming,XU Kun,YU Qin,ZHANG Xiao-yan.Screeningand evaluation of eggplant rootstock for resistance to Meloidogyneincognita[J].Acta Horticulturae Sinica,2008,35(10):1761-1765.徐小明,徐坤,于芹,张晓艳.茄子砧木对南方根结线虫抗性的鉴定与评价[J].园艺学报,2008,35(10):1761-1765.
• [11]JIA Shuang-shuang,GAO Rong-guang,XU Kun.Screening and e-valuation of tomato rootstocks for resistance to Meloidogyne incognita[J].Scientia Agricultura Sinica,2009,42(12):4301-4307.贾双双,高荣广,徐坤.番茄砧木对南方根结线虫抗性鉴定[J].中国农业科学,2009,42(12):4301-4307.
• [12]LIU Wei-zhi.Plant nematology[M].Beijing:China Agriculture Press,2002.刘维志.植物病原线虫学[M].北京:中国农业出版社,2002.
• [13]CHEN Jin-feng,LIN Mao-song,QIAN Chun-tao,ZHUANG Fei-yun,LEWIS S.Identification of Meloidogyne incognita(Kofoid&White)Chitwood resistance in cucumis hystrix Chakr.and the proge-nies of its interspecific hybrid with cucumber(C.Sativus L.)[J].Jour-nal of Nanjing Agricultural University,2001,24(1):21-24.陈劲枫,林茂松,钱春桃,庄飞云,Stephen Lewis.甜瓜属野生种及其与黄瓜种间杂交后代抗根结线虫初步研究[J].南京农业大学学报,2001,24(1):21-24.
• [14]BOITEOX L S,CHARCHAR J M.Genetic resistance to root-knotnematode(Meloidogyne javanica)in eggplant(Solanum melongena)[J].Plant Breeding,1996,115(3):198-200.
• [15]SONG Hong-yuan,LEI Jian-jun,LI Cheng-qiong.Screening andevaluation of plant for reaction and resistance under thermal stress[J].China Vegetable,1998(1):48-50.宋洪元,雷建军,李成琼.植物热胁迫反应及抗性鉴定与评价[J].中国蔬菜,1998(1):48-50.
• [16]LIU Cheng-jing,WANG Chong-qi,JIAO Zi-gao,ZHANG Zhi-zhong,WANG Yan-yan,DONG Yu-mei,XIAO Shou-hua.Effectsof root-knot nematode on growth and quality of watermelon in green-house[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(7):145-148.刘成静,王崇启,焦自高,张志忠,王艳艳,董玉梅,肖守华.根结线虫对日光温室嫁接西瓜生长和品质的影响[J].西北农业学报,2010,19(7):145-148.
• [17]LI Wen-chao,DONG Hui,WANG Xiu-feng.Effects of root-knotnematode on growth,quality and yield of cucumber in greenhouse[J].Journal of Shandong Agricultural University:Natural ScienceEdition,2006,37(1):35-38.李文超,董会,王秀峰.根结线虫对日光温室黄瓜生长、果实品质及产量的影响[J].山东农业大学学报:自然科学版,2006,37(1):35-38.
• [18]GUI Lian-you,MENG Guo-ling,GONG Xin-wen,XIONG San-hao.The cluster analysis of resistantance to Polyphagotarsonemus la-tus(Banks)for eggplant varieties[J].Scientia Agricultura Sinica,2001,34(5):465-468.桂连友,孟国玲,龚信文,熊三浩.茄子品种(系)对侧多食跗线螨抗性聚类分析[J].中国农业科学,2001,34(5):465-468.
• [19]ZHAI Heng,GUAN Xue-qiang,ZHAO Chun-zhi,DU Fang-ling.Screening of chinese grape species resistant to Meloidogyne incogni-ta[J].Acta Horticulturae Sinica,2000,27(1):27-31.翟衡,管雪强,赵春芝,杜方岭.中国葡萄抗南方根结线虫野生资源的筛选[J].园艺学报,2000,27(1):27-31.
• [20]HAO Yu-jin,ZHAI Heng,WANG Shou-hua,PAN Zeng-guang.Resistant screening of apple rootstocks to trichodorus nanjingensis[J].Acta Horticulturae Sinica,1998,25(1):1-5.郝玉金,翟衡,王寿华,潘增光.抗南京毛刺线虫苹果砧木的筛选[J].园艺学报,1998,25(1):1-5.
• [21]ZHOU Guang-sheng,MEI Fang-zhu,ZHOU Zhu-qing,ZHU Xu-tong.Comprehensive evaluation and forecaston physiological indicesof waterlogging resistance of different wheat varieties[J].Scientia A-gricultura Sinica,2003,36(1):1378-1382.周广生,梅方竹,周竹青,朱旭彤.小麦不同品种耐湿性生理指标综合评价及其预测[J].中国农业科学,2003,36(1):1378-1382.
• [22]GAO Qing-hai,XU Kun,GAO Hui-yuan,WU Yan.Screening onchilling tolerance of different eggplant rootstock seedlings[J].Scien-tia Agricultura Sinica,2005,38(5):1005-1010.高青海,徐坤,高辉远,吴燕.不同茄子砧木幼苗抗冷性的筛选[J].中国农业科学,2005,38(5):1005-1010.