8个中国梨品种S基因型的分子鉴定Molecular identification of S-genotypes of Chinese pear cultivars
梁文杰;谭晓风;张琳;曾艳玲;姜傲芳;曹玉芬;李秀根;
摘要(Abstract)：
为鉴定8个中国梨品种的S基因型,使用梨自交不亲和基因(S-RNase)特异引物"FTQQYQ"和"anti-ⅡWP-NV",对8个梨品种的基因组DNA进行特异扩增,并对扩增片段进行回收、克隆、测序。使用生物信息学软件对各序列分析和经同源性搜索分析后,确定了各品种的S基因型。结果分别是:兰州花长把为S19S22,青面为S1S18,黄面为S1S12,早蜜为S19S29,大面黄为S1S19,无子黄为S28S16,大青皮为S34S19及金锤子为S16S19。
关键词(KeyWords)： 梨;自交不亲和性;S基因型;鉴定
基金项目(Foundation): 国家林业局重点科学技术研究计划林业新技术开发与储备专项(2006-12);; 湖南省科技三项基金(编号:00JZYZ115)
作者(Author): 梁文杰;谭晓风;张琳;曾艳玲;姜傲芳;曹玉芬;李秀根;
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