李琳玲;程华;许锋;王燕;常杰;程水源;

• 1:河北农业大学园艺学院
• 2:黄冈师范学院生命科学与工程学院
• 3:长江大学园艺园林学院

摘要(Abstract)：

利用RACE技术从银杏中克隆到叶绿体铁型超氧化物歧化酶(GbFeSOD)基因cDNA全长。GbFeSOD的cDNA全长1106bp(GenBank:FJ555019),含有一个720bp的最大读码框。三维结构预测显示,GbFeSOD含有11个α螺旋和3个β折叠构成的篮子状活性中心。GbFeSOD氨基酸序列与其他植物的FeSOD具有很高的相似性。进化树分析表明GbFeSOD和其他物种起源相同。Southern杂交显示,GbFeSOD属于一个小的多基因家族。Northern杂交表明GbFeSOD在银杏的茎、叶中的表达量最高,果中表达量最小,在根中没有表达。ABA、渗透物质处理、低温和高温均能诱导GbFeSOD表达量增加,其中4℃低温诱导增加缓慢,40℃高温诱导迅速而稳定,而44℃培养条件下,GbFeSOD表达量呈先增后降的现象。

关键词(KeyWords)： 银杏;FeSOD基因;表达分析

Abstract:

Keywords:

基金项目(Foundation): 教育部新世纪优秀人才支持计划(NCET-04-0746);; 湖北省教育厅重大科技项目(Z200627002);; 湖北省青年杰出人才基金(2003AB014)

作者(Author): 李琳玲;程华;许锋;王燕;常杰;程水源;

Email:

DOI:

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