张颖;樊秀彩;姜建福;李民;刘崇怀;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】在前期的种质资源评价过程中,发掘一株中国野生刺葡萄0943,该株系高抗葡萄白腐病。基于mi-croRNA(miRNA)在植物抗病中的重要作用,拟从miRNA水平探讨刺葡萄在受到病原菌侵染后的表达调控机制。【方法】以抗病的中国野生刺葡萄为试材,对比感病的欧亚种'美人指’,分别以病原菌诱导,在0 hpi(hours post inoculation)和病菌诱导后的12 hpi、36 hpi处理后采样,进行二代测序,并对数据进行KEGG及表达量的差异分析。【结果】对比感病葡萄'美人指’,分析了抗病刺葡萄在基础代谢和抗病途径中的差异,结合miRNA的表达量,获得了150个表达量发生变化的miRNA,其中44个miRNA的表达在刺葡萄和'美人指’之间存在差异。5个miRNA在刺葡萄中特异表达,但是在'美人指’中不表达,实施定量验证了这一结果。靶基因预测显示,其靶基因包括与抗病的紧密相关的WRKY、SPL、EFR等转录因子,还包括与抗病直接相关的LRR类的抗病基因。【结论】筛选出5个在刺葡萄上特异表达候选miRNA(miR172a、miR172b、miR845a、novel_81和miR159a),可作为刺葡萄抗白腐病研究的目标。

关键词(KeyWords)： 葡萄白腐病;microRNA;抗病;测序

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(31872057);; 中国农业科学院科技创新工程(CAAS-ASTIP-2018-ZFRI);; 现代农业产业技术体系建设专项资金(CARS-29-yz-1);; 中央级科研院所基本科研业务费;; 农业部作物种质资源保护项目(NB2130135-34)

作者(Author): 张颖;樊秀彩;姜建福;李民;刘崇怀;

Email:

DOI: 10.13925/j.cnki.gsxb.20180165

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