树莓炭疽病病原菌鉴定Identification of the pathogen causing anthracnose of raspberry
戴启东;李广旭;杨华;张广仁;张秉宇;
摘要(Abstract)：
【目的】为了明确引起树莓炭疽病的病原,【方法】从30份标样中采用组织分离法分离得到了18个代表性菌株,并随机选取一个菌株LN-14,对该菌株的形态学特征、菌落特性以及病菌的致病性进行了研究。采用真菌rDNA-ITS区域通用引物ITS1和ITS4对菌株进行PCR扩增,运用DNAMAN软件构建该菌株与其他近缘种类菌株的系统发育树。【结果】该菌在PDA培养基上菌落呈灰白色,产生黑色分生孢子盘,分生孢子无色、单孢子呈椭圆形12～19μm×4～5μm。PCR扩增得到534 bp的特异性片段,测序、比对结果显示该菌与Colletotrichum gloeosporioides[HQ264183]、C.gloeosporioides[HQ264182]的相似性高达100%,与引起杧果炭疽病的C.gloeosporioides[JX560098]亲缘关系较近聚类为同一个分支,而与草莓炭疽病菌C.fragariae[JN943084]亲缘关系较远。【结论】形态学和分子鉴定表明辽宁地区树莓炭疽病的病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides)。
关键词(KeyWords)： 树莓;炭疽病;病原鉴定;胶孢炭疽菌
基金项目(Foundation):
作者(Authors): 戴启东;李广旭;杨华;张广仁;张秉宇;
DOI: 10.13925/j.cnki.gsxb.2013.04.008
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