牛姣,张玉成,郭荣荣,王西平

• 1:西北农林科技大学园艺学院·农业部西北地区园艺作物生物学与种质创制重点开放实验室
• 2:旱区作物逆境生物学国家重点实验室
• 3:西北农林科技大学葡萄酒学院
• 4:陕西省葡萄与葡萄酒工程技术研究中心

摘要(Abstract)：

【目的】为了验证SAP基因在葡萄抗病防御机制中的作用,【方法】在前期通过抑制消减杂交技术(SSH)获得中国野生葡萄毛葡萄株系‘商-24’衰老相关蛋白(SAP)基因EST序列的基础上,采用RT-PCR从毛葡萄‘商-24’中分离到SAP基因cDNA序列,并通过实时定量PCR和半定量PCR分析了SAP基因的表达特异性。【结果】序列分析表明该基因具有完整的的开放阅读框,序列长度为819 bp,编码272个氨基酸残基,相对分子质量为30.56 ku,等电点为8.78,将其命名为VqSAP。多重比较结果显示VqSAP基因与油棕、玉米、萱草、拟南芥、水稻等植物SAP基因的同源性为65%~74%。实时定量结果表明,在白粉菌诱导初期,抗病的毛葡萄株系‘商-24’和感病的华东葡萄株系‘湖南-1’VqSAP基因表达量均升高,这可能是因为它参与了病原菌诱导的细胞程序性死亡过程。水杨酸(SA)、茉莉酸甲酯(MeJA)、乙烯(Eth)处理表明衰老相关蛋白基因表达受激素分子调节,但在抗病和感病株系中基因的表达量有所差异。在嫩叶、嫩茎、花、果皮、卷须等不同葡萄组织中的表达表明VqSAP基因的表达具有空间差异性。【结论】这些结果暗示着VqSAP的表达响应病原菌侵染并受生物胁迫相关激素的调控,很有可能在葡萄防御病原菌侵染的机制中发挥重要作用。

关键词(KeyWords)： 毛葡萄;衰老相关蛋白;VqSAP基因;克隆;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30871701,31071782)

作者(Author): 牛姣,张玉成,郭荣荣,王西平

DOI: 10.13925/j.cnki.gsxb.2012.04.015

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