焦朝霞;邵建柱;孙建设;

• 1:河北农业大学园艺学院

摘要(Abstract)：

针对田间苹果成熟组织富含多酚、多糖和大量次生代谢产物的特点,建立了适合田间苹果不同组织的高效RNA提取方法,以不同季节田间生长的苹果叶片和皮层为材料,用此方法均能在2 h左右完成超过10个样品的总RNA提取,质量高、完整性好的总RNA,可用于田间苹果病毒RT-PCR检测,获得良好效果。经反复大量试验证实,该方法适合田间苹果不同组织的RNA快速提取,操作简便、快捷、高效,且使其不受季节限制,有效地提高了田间果树病毒检测以及其他分子生物学研究的效率。

关键词(KeyWords)： 田间苹果;成熟组织;RNA;高效提取

Abstract:

Keywords:

基金项目(Foundation): 农业部公益性行业科研专项(nyhyzx07-024);农业部国家现代苹果产业技术体系功能岗位专家项目(08-01-02-03)

作者(Author): 焦朝霞;邵建柱;孙建设;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.01.025

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