张敏;周彩红;陈焘;戴文圣;张迟;

• 1:浙江农林大学·省部共建亚热带森林培育国家重点实验室
• 2:景宁嘉树农业科技开发有限公司

摘要(Abstract)：

【目的】基于‘细榧’(Torreya grandis‘Xifei’)和‘圆榧’(T.grandis‘Dielsii’)种子转录组数据,开发SSR分子标记,为榧属植物遗传多样性研究奠定基础。【方法】利用MISA软件对筛选得到的1 kb以上的Unigene做SSR分析,利用Primer 3.0设计引物,随机选择49对引物进行多态性扩增。【结果】在22 976条评估数目中,包含SSR位点5 458个,共鉴定出6种类型的SSR。单核苷酸重复、三核苷酸重复和二核苷酸重复分别占SSR总数的64.9%、18.56%和14.77%。对5 458个SSR位点设计出4 633对SSR引物,随机选择49对在10份榧属植物中进行多态性扩增,其中16对引物表现出多态性,各个位点的等位基因数为2⁶个,平均等位基因数3个,多态信息含量PIC、观测杂合度Ho、期望杂合度He的平均值分别是0.464 4、0.283 7和0.500 6。【结论】榧树转录组测序数据能够提供丰富的SSR位点,用于快速高效地开发SSR引物。所开发的引物能为榧树遗传多样性研究、品种指纹图谱构建和分子标记辅助选择育种提供标记选择。

关键词(KeyWords)： 榧树;转录组;SSR

Abstract:

Keywords:

基金项目(Foundation): 林业公益性行业科研专项(201504708);; 浙江省团队科技特派员服务计划项目(浙科发农[2013]215-122);; 浙江省科技计划项目(2012C22095);; 浙江省农业新品种选育重大科技专项(2016C02052-12)

作者(Authors): 张敏;周彩红;陈焘;戴文圣;张迟;

DOI: 10.13925/j.cnki.gsxb.20170034

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