猕猴桃雌雄性别的AFLP鉴别中DNA模板的制备Making DNA Template in the Study of Sex Determination in Actinidia by AFLP
张潞生,李传友,贾建航,李绍华,王斌,肖兴国
摘要(Abstract):
基因组DNA的成功制备和避免降解是AFLP成功的关键。描述了猕猴桃AFLP研究中的DNA模板制备方法,尤其是DNA的提取和纯化,以及基因组DNA的酶切中常见的问题及其相应的解决措施。应用这些方法得到了猕猴桃清晰的AFLP指纹图谱。
关键词(KeyWords): 猕猴桃;AFLP;DNA模板
基金项目(Foundation): 欧盟、国家教委资助
作者(Author): 张潞生,李传友,贾建航,李绍华,王斌,肖兴国
Email:
DOI: 10.13925/j.cnki.gsxb.1999.03.003
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