林苗苗;职倩倩;陈锦永;顾红;张威远;张洋;方金豹;

• 1:中国农业科学院郑州果树研究所·果树生长发育与品质控制重点开放实验室

摘要(Abstract)：

【目的】探明在高温下甜樱桃胚囊败育的原因。【方法】以甜樱桃品种‘早红宝石’(Prunus avium L.‘Zao Hong Bao Shi’)为试材,利用透射电镜分别对露地和日光温室条件下露白期、开花前1 d和开花后1 d胚珠中淀粉和细胞器进行超微结构观察。【结果】(1)发现淀粉颗粒仅存在于珠被上,在珠心细胞中未观察到淀粉颗粒;(2)从花芽露白期到开花后,露地和日光温室‘早红宝石’的胚珠细胞中淀粉粒在逐渐减少,同时线粒体数量在增多且结构越来越完整,畸形、嵴模糊或消失现象在好转;(3)在同一发育时期,露地‘早红宝石’珠被细胞中淀粉粒数量上少于温室的,珠心细胞线粒体数量及结构完整性不及温室的;(4)在珠被细胞中,部分线粒体嵴腔中存在淀粉微粒。【结论】胚囊发育所需的能量可能由珠被淀粉水解后供给,并且珠被的完整性对于胚囊的发育至关重要。

关键词(KeyWords)： 甜樱桃;胚珠;淀粉;细胞器;超微结构

Abstract:

Keywords:

基金项目(Foundation): 中国农科院果树生长发育与品质控制重点开放实验室项目(PP333KF20090101);; 河南省大宗水果产业技术体系

作者(Author): 林苗苗;职倩倩;陈锦永;顾红;张威远;张洋;方金豹;

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参考文献(References)：

• [1]LI Yan,LI Ling,LI Shao-xuan,WANG Hui,YE Bao-xing,GAO Dong-sheng.Effect of high temperature on the flower development of sweet cherry in solar greenhouse[J].Scientia Agricultura Sinica,2011,44(10):2101-2108.李燕,李玲,李少旋,王慧,叶宝兴,高东升.高温对设施甜樱桃花器官发育的影响[J].中国农业科学,2011,44(10):2101-2108.
• [2]BEPPU K,IKUO K.Studies on Pistil Doubling and Fruit Set of Sweet Cherry in Warm Climate[J].J Japan Soc Hort Sci,2011,80(1):1-13.
• [3]WANG Xi,GOU Xiao-xia,WANG Jian-jun.Review on molecular mechanism and genetic regulation of female gametophyte development in plant[J].Fen Zi Zhiwu Yuzhong,2012,10:1067-1079.王曦,勾晓霞,王建军.植物雌配子体发育的分子机理与基因调控[J].分子植物育种,2012,10:1067-1079.
• [4]HU Shi-yi,YANG Hong-yuan.Biology of angiosperm fertilization[M].Beijing:Science Press,2002:83-85.胡适宜,杨弘远.被子植物受精生物学[M].北京:科学出版社,2002:83-85.
• [5]TIAN Hui-qiao.The change of starch grain during embryo sac development in Oryza sativa L.[J].Acta Phytophysiologia Sinica,1984,10(1):97-102.田惠桥.水稻胚囊发育过程中淀粉粒的变化[J].植物生理学报,1984,10(1):97-102.
• [6]WEI Cun-xu,ZHANG Jun,ZHOU Wei-dong,CHEN Yi-fang,XU Ru-gen.Development of small starch granule in barley endosperm[J].Acta Agronomica Sinica,2008,34(10):1788-1796.韦存虚,张军,周卫东,陈义芳,许如根.大麦胚乳小淀粉粒的发育[J].作物学报,2008,34(10):1788-1796.
• [7]ZHI Qian-qian,ZHAO Chang-zhu,GU Hong,LIN Miao-miao,CHEN Jin-yong,YANG Xiao-hong,FANG Jin-bao.Observation of the development characteristics of floral bud and localization of polysaccharides in the ovule of sweet cherry in field and green house[J].Journal of Fruit Science,2012,29(3):466-470.职倩倩,赵长竹,顾红,林苗苗,陈锦永,杨晓红,方金豹.露地和日光温室甜樱桃花芽发育特征及胚珠多糖定位观察[J].果树学报,2012,29(3):466-470.
• [8]YOU Rui-lin.An Ultrastructural study of nucellar cells in Wheat(Triticum aestivum L.)[J].Acta Biologiae Experimentalis Sinica,1985,18(4):369-377.尤瑞麟.小麦珠心细胞的超微结构研究[J].实验生物学报,1985,18(4):369-377.
• [9]YANG Shi-jie.Plant biology[M].Beijing:Science Press,2000.杨世杰.植物生物学[M].北京:科学出版社,2000.
• [10]LUO Yu-ying.Ultrastructural study on the division of amyloplast in pollen grain of Polygonatum simizui[J].Journal of Chinese Electron Microscopy Society,1995,14:166-169.罗玉英.玉竹花粉中淀粉质体形成及增殖的超微结构研究[J].电子显微学报,1995,14:166-169.
• [11]BAI Shi-ping,LUO Xu-gang,LLin.Mitochondrial intervention action in apoptosis[J].Chinese Bulletin of Life Sciences,2008,18(4):368-372.白世平,罗绪刚,吕林.线粒体在细胞凋亡中的介导作用[J].生命科学,2008,18(4):368-372.
• [12]BIAN Wei-dong,DENG Yan-hua,ZHU Yu-xian,QIN Rui,CHEN Yu-lin.Study on the relationship between the development of ovule and pollen of sweet cherry and the effect of temperature[J].Journal of Fruit Science,2006,23(4):609-612.边卫东,邓艳华,朱育贤,秦瑞,陈玉林.大樱桃胚珠、花粉发育与温度变化的关系[J].果树学报,2006,23(4):609-612.
• [13]BIAN Wei-dong.Characterization of the flowering and fruit set of sweet cherry in greenhouse[D].Yangling:Northwest A&F University,2008.边卫东.温室甜樱桃开花结实特性研究[D].杨凌:西北农林科技大学,2008.
• [14]LI Rui,LAN Sheng-yin,XU Zhen-xiu.Transformation of mitochondrion to amyloplast and the cytochemical localization of succinic dehydrogenase in rice endosperm[J].Journal of Chinese Electron Microscopy Society,2002,4:118-122.李睿,蓝盛银,徐珍秀.水稻胚乳发育中线粒体向淀粉质体转变及琥珀酸脱氢酶的定位[J].电子显微学报,2002,4:118-122.
• [15]WEI Cun-xu,LAN Sheng-yin,XU Zhen-xiu.Ultrastructural features of nucleus degradation during Programmed cell death of starchy endosperm cells in rice[J].Acta Botanica Sinica,2002,44(12):1396-1402.韦存虚,蓝盛银,徐珍秀.水稻淀粉胚乳细胞编程性死亡中细胞核变化特征[J].植物学报,2002,44(12):1396-1402.
• [16]WEI Cun-xu,LAN Sheng-yin,XU Zhen-xiu.Relation between envelope and proliferation of amyloplast in endosperm cell of rice[J].Journal of Chinese Electron Microscopy Society,2002,12(2):123-128.韦存虚,蓝盛银,徐珍秀.水稻胚乳细胞淀粉质体被膜与其增殖的关系[J].电子显微学报,2002,12(2):123-128.
• [17]LAN Sheng-yin,XU Zhen-xiu.Submicrostructure research of rice endosperm starch grains morphological development[J].Journal of Wuhan University,1997,8:73-74.蓝盛银,徐珍秀.水稻胚乳淀粉粒形态发育的亚显微结构研究[J].武汉大学学报,1997,8:73-74.
• [18]SUN De-lan,SUN Xiu-zhen,WANG Hong.Observation on the Amyloplasts development and Biomacromolecules of plastid in developing cotyledon of cells of Lotus[J].Acta Botanica Boreall-occidentalia Sinica,1998,18(4):570-576.孙德兰,苏秀珍,王红.莲幼胚子叶细胞中淀粉质体的发育及其生物大分子的观察[J].西北植物学报1998,18(4):570-576.