黎维丽;余乃通;李俊成;王健华;李宾宾;刘志昕;

• 1:海南大学热带农林学院
• 2:中国热带农业科学院热带生物技术研究所·海南省微生物学重点实验室·农业农村部热带作物生物学与遗传资源利用重点实验室
• 3:广东省农业科学院果树研究所·广东省热带亚热带果树研究重点实验室

摘要(Abstract)：

【目的】探明杆状DNA病毒属病毒的ORFⅠ基因功能。【方法】本研究从香蕉条斑病毒云南分离株(BSV-YN)中克隆了BSV ORFⅠ基因,通过核苷酸和氨基酸序列分析和同源关系分析,确定了BSV-YN的分类地位;对其编码蛋白及同源物的理化性质、氨基酸序列组成、亲疏水性、跨膜区域、信号肽、亚细胞定位、磷酸化位点、同源结构域、蛋白二级和三级结构等进行预测和分析。【结果】BSV ORFⅠ基因片段大小为528 bp,编码175个氨基酸,通过核苷酸和氨基酸序列分析和同源关系分析,确定了BSV-YN属于BSGFV种。生物信息学分析表明,BSV-YN ORFⅠ及同源蛋白都是不稳定的、亲水性蛋白,以丝氨酸和苏氨酸磷酸化为主。这些蛋白二级结构主要由α-螺旋和无规则卷曲组成,无信号肽或跨膜区域。亚细胞定位预测表明,BSV-YN ORFⅠ及同源蛋白主要在细胞核分布,推测它们在病毒感染的宿主细胞核中发挥重要作用。【结论】推测BSV-YN ORFⅠ在病毒感染的宿主细胞核中参与病毒复制、转录等相关过程。本研究为进一步开展该蛋白的功能研究提供科学线索。

关键词(KeyWords)： 香蕉条斑病毒;ORFⅠ;同源蛋白;生物信息学分析

Abstract:

Keywords:

基金项目(Foundation): 广东省热带亚热带果树研究重点实验室开放基金(2017B030314113);; 中国热带作物学会青年人才托举工程(CSTCQN201704);; 中国热带农业科学院基本科研业务费专项(No.19CXTD-33)

作者(Author): 黎维丽;余乃通;李俊成;王健华;李宾宾;刘志昕;

Email:

DOI: 10.13925/j.cnki.gsxb.20180490

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