孙计平;张玉星;李英丽;张江红;张召;

• 1:河北农业大学园艺学院
• 2:河南省农业科学院烟草研究所

摘要(Abstract)：

【目的】探讨生草对梨园土壤生物学特性的影响。【方法】以清耕为对照,研究冀中生草4 a和生草8 a梨园,在0~80 cm土层内分层取样,测定土壤微生物、酶活性和腐殖质含量,研究生草对梨园土壤生物特性的影响。【结果】梨园微生物以细菌为主,与清耕相比较,生草可以显著增加0~20 cm土层细菌数量,且随着生草年限增加,效果显著,生草8 a可以显著提高0~30 cm土层细菌、0~50 cm土层真菌和0~20 cm土层放线菌数量;生草8 a 0~10 cm土层脲酶和过氧化氢酶活性显著提高;生草可以显著提高0~10 cm土层胡敏酸含量,且随着生草年限增加,胡敏酸含量升高,生草8 a 0~20 cm土层胡敏酸与富里酸比值显著高于清耕,土壤稳定性好。【结论】长期生草可以有效增加表层土壤微生物数量,提高土壤脲酶活性,增加土壤腐殖酸含量,使土壤腐殖质品质朝好的方向转化,有利于梨园土壤可持续发展。

关键词(KeyWords)： 梨园;生草;土壤微生物;酶活性;腐殖酸

Abstract:

Keywords:

基金项目(Foundation): 国家梨产业技术体系资助项目(CARS-29-13)

作者(Author): 孙计平;张玉星;李英丽;张江红;张召;

Email:

DOI: 10.13925/j.cnki.gsxb.2016.S.18

参考文献(References)：

• [1]谢凯,宋晓晖,董彩霞,徐阳春.不同有机肥处理对黄冠梨生长及果园土壤性状的影响[J].植物营养与肥料学报,2013,19(1):214-222.XIE Kai,SONG Xiaohui,DONG Caixia,XU Yangchun.Effects of different organic fertilizers on tree growth and soil property in Huangguan pear orchard[J].Plant Nutrition and Fertilizer Science,2013,19(1):214-222.
• [2]周少燕,赵慧峰,张存彦,吕润航,刘杏访.河北省不同栽培模式的梨果生产劳动力成本分析[J].湖北农业科学,2012,51(10):2157-2160.ZHOU Shaoyan,ZHAO Huifeng,ZHANG Cunyan,LU Runhang,LIU Xingfang.Analysis on pear production labor costs with different cultivation patterns in Hubei province[J].Hubei Agricultural Sciences,2012,51(10):2157-2160.
• [3]刘建新,王鑫,杨建霞.覆草对果园土壤腐殖质组成和生物学特性的影响[J].水土保持学报,2005,19(4):93-95.LIU Jianxin,WANG Xin,YANG Jianxia.Effects of covering straw in orchard on humus composition and biological characteristics[J].Journal of Soil and Water Conservation,2005,19(4):93-95.
• [4]曹艳峰,李彦,李晨华,吕光辉.荒漠灌木梭梭土壤微生物的空间分布[J].生态学报,2016,36(6):1-8.CAO Yanfeng,LI Yan,LI Chenhua,LU Guanghui.The spatial distribution of soil microbes around a desert shrub of Haloxylon ammodendron[J].Acta Ecologica Sinica,2016,36(6):1-8.
• [5]刘富庭,张林森,李雪薇,李丙智,韩明玉,谷洁,王晓琳.生草对渭北旱地苹果园土壤有机碳组分及微生物的影响[J].植物营养与肥料学报,2014,20(2):355-363.LIU Futing,ZHANG Linsen,LI Xuewei,LI Bingzhi,HAN Mingyu,GU Jie,WANG Xiaolin.Effects of inter-row planting grasses on soil organic carbon fractions and soil microbial community of apple orchard in Weibei dryland[J].Plant Nutrition and Fertilizer Science,2014,20(2):355-363.
• [6]李会科.渭北旱地苹果园生草的生态环境效应及综合技术体系构建[D].杨凌:西北农林科技大学,2008:62-67.LI Huike.Eco-environmental effect and integrated technical system of green cover in apple orchard in weibei dryland farming areas[J].Yangling:Northwest A&F University,2008:62-67.
• [7]惠竹梅,岳泰新,张瑾,程建梅,李华.西北半干旱区葡萄园生草体系中土壤生物学特性与土壤养分的关系[J].中国农业科学,2011,44(11):2310-2317.XI Zhumei,YUE Taixin,ZHANG Jin,CHENG Jianmei,LI Hua.Relationship between soil biological characteristics and nutrient content under grass intercropping system of vineyard in northwestern semiarid area[J].Scientia Agricultura Sinica,2011,44(11):2310-2317.
• [8]钱进芳,吴家森,黄坚钦.生草栽培对山核桃林地土壤养分及微生物多样性的影响[J].生态学报,2014,34(15):4324-4332.QIAN Jinfang,WU Jiasen,HUANG Jianqin.Effects of sod-cultural practices on soil nutrients and microbial diversity in the Carya cathayensis forest[J].Acta Ecologica Sinica,2014,34(15):4324-4332.
• [9]李国怀,章文才,刘继红,胡德文.柑桔园生草栽培的生态效应研究[J].生态学杂志,1997,16(6):6-11.LI Guohuai,ZHANGWencai,LIU Jihong,HU Dewen.Studies of ecological effects of sod culture in citrus orchard[J].Chinese Journal of Ecology,1997,16(6):6-11.
• [10]霍颖,张杰,王美超,姚允聪.梨园行间种草对土壤有机质和矿质元素变化及相互关系的影响[J].中国农业科学,2011,44(7):1415-1424.HUO Ying,ZHANG Jie,WANG Meichao,YAO Yuncong.Effects of inter-row planting grasses on variations and relationships of soil organic matter and soil nutrients in pear orchard[J].Scientia Agricultura Sinica,2011,44(7):1415-1424.
• [11]王艳廷,冀晓昊,吴玉森,毛志泉,姜远茂,彭福田,王志强,陈学森.我国果园生草的研究进展[J].应用生态学报,2015,26(6):1892-1900.WANG Yanting,JI Xiaohao,WU Yusen,MAO Zhiquan,JIANG Yuanmao,PENG Futian,WANG Zhiqiang,CHEN Xuesen.Research progress of cover crop in chinese orchard[J].Chinese Journal of Applied Ecology,2015,26(6):1892-1900.
• [12]孙计平,张玉星,吴照辉,李英丽,张召.生草对梨园土壤物理特性的影响[J].水土保持学报,2015,29(5):194-199.SUN Jiping,ZHANG Yuxing,WU Zhaohui,LI Yingli,ZHANG Zhao.Effects of planting herbage on soil physical properties of pear orchard[J].Journal of Soil and Water Conservation,2015,29(5)194-199.
• [13]吴玉森,张艳敏,冀晓昊,张芮,刘大亮,张宗营,李文燕,陈学森.自然生草对黄河三角洲梨园土壤养分、酶活性及果实品质的影响[J].中国农业科学,2013,46(1):99-108.WU Yusen,ZHANG Yanmin,JI Xiaohao,ZHANG Rui,LIU Daliang,ZHANG Zongying,LI Wenyan,CHEN Xuesen.Effects of natural grass on soil nutrient,Enzyme activity and fruit quality of pear orchard in yellow river delta[J].Scientia Agricultura Sinica,2013,46(1):99-108.
• [14]付学琴,刘琚珥,黄文新.南丰蜜橘园自然生草对土壤微生物和养分及果实品质的影响[J].园艺学报,2015,42(8):1551-1558.FU Xueqin,LIU Juer,HUANG Wenxin.Effects of natural grass on soil microbiology,nutrient,fruit quality of Nanfeng Tangerine yard[J].Acta Horticulturae Sinica,2015,42(8):1551-1558.
• [15]中国科学院南京土壤研究所微生物室.土壤微生物研究法[M].北京:科学出版社,1985:54-59.Microbiology Laboratory of Nanjng Institute of soil science,Chinese Academy of Sciences.Soil microbial research method[M].Beijing:Science Press,1985:54-59.
• [16]周礼恺.土壤酶学[M].北京:科学出版社,1987.ZHOU Likai.Soil enzyme[M].Beijing:Science Press,1987.
• [17]KUMADA K,SATO O,OHSUMI Y.Humus composition of mountain soils in central Japan with special reference to the distribution of P type humic acid[J].Soil Science and Plant Nutrition,1967,13(5):151-158.
• [18]TANG Q Y,ZHANG C X.Data Processing System(DPS)software with experimental design,statistical analysis and data mining developed for use in entomological research[J].Insect Science,2013,20(2):254-260.
• [19]谢凯,李元军,乐文全,王少敏,李俊才,董彩霞,徐阳春.环渤海湾地区主要梨园土壤养分状况及养分投入研究[J].土壤通报,2013,44(1):132-137.XIE Kai,LI Yuanjun,LE Wenquan,WANG Shaomin,LI Juncai,DONG Caixia,XU Yangchun.A study on soil nutrient status and nutrients input in the main pear orchards around Bohai bay region[J].Chinese Journal of Soil Science,2013,44(1):132-137.
• [20]龙妍,惠竹梅,程建梅,庞学良.生草葡萄园土壤微生物分布及土壤酶活性研究[J].西北农林科技大学学报(自然科学版)2007,35(6):99-103.LONG Yan,XI Zhumei,CHENG Jianmei,PANG Xueliang.Ecological distributing of soil microorganisms and activity of soil enzymes in vineyard green covering[J].Journal of Northwest A&F University,2007,35(6):99-103.
• [21]李艳丽,赵化兵,谢凯,宋晓晖,徐阳春,董彩霞.不同土壤管理方式对梨园土壤微生物及养分含量的影响[J].土壤,2012,44(5):788-793.LI Yanli,ZHANG Huabing,XIE Kai,SONG Xiaohui,XU Yangchun,DONG Caixia.Effects of different soil managements on pear orchard soil biological properties and nutrient contents[J].Soils,2012,44(5):788-793.
• [22]刘亚柏,王敬根,付反生,储国良.果园生草栽培对土壤生态环境的影响[J].扬州大学学报(农业与生命科学版),2015,36(4):110-113.LIU Yabo,WANG Jinggen,FU Fansheng,CHU Guoliang.Effect of grass planting in orchard on soil ecological environment[J].Journal of Yangzhou University(Agricultural and Life Science Edition),2015,36(4):110-113.
• [23]王灿,王德建,孙瑞娟,林静慧.长期不同施肥方式下土壤酶活性与肥力因素的相关性[J].生态环境,2008,17(2):688-692.WANG Can,WANG Dejian,SUN Ruijuan,LIN Jinghui.The relationship between soil enzyme activities and soil nutrients by longterm fertilizer experiments[J].Ecology and Environment,2008,17(2):688-692.
• [24]BANDICK A K,DICK R P.Field management effects on soil enzyme activities[J].Soil Biology and Biochemistry,1999,31:1471-1479.
• [25]龚伟,颜晓元,王景燕,胡庭兴,宫渊波.长期施肥对小麦-玉米作物系统土壤腐殖质组分碳和氮的影响[J].植物营养与肥料学报,2009,15(6):1245-1252.GONG Wei,YAN Xiao,WANG Jingyan,HU Tingxing,GONG Yuanbo.Effects of long-term fertilization on soil humus carbon and nitrogen fractions in a wheat-maize cropping system[J].Plant Nutrition and Fertilizer Science,2009,15(6):1245-1252.
• [26]张晓曦,刘增文,朱博超,邴塬皓,于齐,Luo Nhu Trung.半干旱黄土丘陵区纯林土壤腐殖质异化特征及与其他性质的关系[J].生态学报,2015,35(6):1779-1787.ZHANG Xiaoxi,LIU Zengwen,ZHU Bochao,BING Yuanhao,YU Qi,Luo Nhu Trung.Humus differentiation and correlation with other soil properties under pure forest in semi-arid Loess Hilly Area[J].Acta Ecologica Sinica,2015,35(6):1779-1787.
• [27]李海波,韩晓增,王风,乔云发.不同土地利用下黑土密度分组中碳、氮的分配变化[J].土壤学报,2008,45(1):112-119.LI Haibo,HAN Xiaozeng,WANG Feng,QIAO Yunfa.Distribution of soil organic carbon and nitrogen in density fractions on black soil as affected by land use[J].Acta Pedologica Sinica,2008,45(1):112-119.
• [28]褚慧,宗良纲,汪张懿,谢少华,杨旎,罗敏.不同种植模式下菜地土壤腐殖质组分特性的动态变化[J].土壤学报,2013,50(5):931-939.CHU Hui,ZONG Lianggang,WANG Zhangyi,XIE Shaohua,YANG Ni,LUO Min.dynamic changes in humus composition in vegetable soils different in cultivation mode[J].Acta Pedologica Sinica,2013,50(5):931-939.