陈健美;谢丽红;周娟;江小美;钟八莲;李淑惠;杨斌华;喻芳琴;

• 1:赣南师范大学·国家脐橙工程技术研究中心
• 2:于都县农业与粮食局
• 3:于都县果茶局

摘要(Abstract)：

【目的】明确早熟品种‘赣南早’脐橙(wild type,WT)与其早熟性状回复型突变体(mutant type, MT)在生理和转录水平的差异,探究柑橘果实成熟的调控机制。【方法】测定MT和WT的果实品质及成熟相关生理指标,采用RNASeq分析MT和WT果实的转录差异。【结果】MT具有稳定的早熟性状回复现象。MT和WT果皮的各可溶性糖含量差异显著。MT和WT有机酸含量在果肉间及果皮间差异均显著。MT果皮的赤霉素(gibberellin,GA)、吲哚乙酸(indole acetic acid,IAA)和茉莉酸(jasmonic acid,JA)显著高于WT,脱落酸(abscisic acid,ABA)则相反。转录组测序分析表明,MT与WT果皮间DEGs数量为980个,果肉间为289个。在果皮DEGs中,有38种GO分类、6个KEGG代谢通路被显著性富集,而果肉中未见GO分类和KEGG代谢通路的显著性富集。ABA合成基因CsNCED1在MT果皮和果肉中均下调表达,而分解基因CsCYP707A1在MT果皮中上调表达。【结论】MT成熟期比WT推迟约30 d。MT果皮GA含量及GA合成基因CsCPS1、CsKAO表达量均高于WT,可能与果皮的褪绿延迟相关。MT果皮ABA积累抑制可能受合成基因CsNCED1下调表达及分解基因CsCYC707A1上调表达的影响。MT和WT果皮的生理和转录组水平差异均比果肉间差异大,说明果皮在柑橘果实成熟过程中具有重要作用。

关键词(KeyWords)： ‘赣南早’脐橙;回复型突变;果实成熟;果实品质;转录组

Abstract:

Keywords:

基金项目(Foundation): 江西省科技重大项目(20152ACF60007);; 江西省青年基金项目(20132BAB2014018);; 江西省科技支持项目(20121BBF60023,20132BBF60026);; 2013年中央财政农业科技推广专项资金

作者(Author): 陈健美;谢丽红;周娟;江小美;钟八莲;李淑惠;杨斌华;喻芳琴;

Email:

DOI: 10.13925/j.cnki.gsxb.20170463

参考文献(References)：

• [1] PAN X,ZHU B,ZHU H,CHEN Y,TIAN H,LUO Y,FU D.iTRAQ protein profile analysis of tomato green-ripe mutant reveals new aspects critical for fruit ripening[J]. Journal of Proteome Research,2014,13(4):1979.
• [2] ZENG J,GAO C,DENG G,JIANG B,YI G,PENG X,ZHONG Y,ZHOU B,LIU K. Transcriptome analysis of fruit development of a Citrus late-ripening mutant by microarray[J].Scientia Horticulturae,2012,134(2):32-39.
• [3] SUN L,WANG Y P,CHEN P,REN J,JI K,LI Q,LI P,DAI S J,LENG P. Transcriptional regulation of SlPYL,SlPP2C,and SlSnRK2 gene families encoding ABA signal core components during tomato fruit development and drought stress[J]. Journal of Experimental Botany,2011,62(15):5659.
• [4] JIA H F,LU D,SUN J H,LI C L,XING Y,QIN L,SHEN Y Y.Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening[J]. Journal of Experimental Botany,2013,64(6):1677-1687.
• [5] NICOLAS P,LECOURIEUX D,KAPPEL C,CLUZET S,CRAMER G R,DELROT S,LECOURIEUX F. The bZIP transcription factor VvABF2 is an important transcriptional regulator of ABA-dependent grape berry ripening processes[J]. Plant Physiology,2013,35(5):523-525.
• [6] KARPPINEN K,HIRVEL?E,NEVALA T,SIPARI N,SUOKAS M,JAAKOLA L. Changes in the abscisic acid levels and related gene expression during fruit development and ripening in bilberry(Vaccinium myrtillus L.)[J]. Phytochemistry,2013,95(6):127.
• [7] JIA H,LI C,CHAI Y,XING Y,SHEN Y. Sucrose promotes strawberry fruit ripening by stimulation of abscisic acid biosynthesis[J]. Pakistan Journal of Botany,2013,45(1):169-175.
• [8] CONCHA C M,FIGUEROA N E,POBLETE L A,O?ATE F A,SCHWAB W,FIGUEROA C R. Methyl jasmonate treatment induces changes in fruit ripening by modifying the expression of several ripening genes in Fragaria chiloensis fruit[J]. Plant Physiology&Biochemistry,2013,70(1):433-444.
• [9]蒋天梅,殷学仁,王平,孙崇德,徐昌杰,李鲜,陈昆松.乙烯调控非跃变型果实成熟衰老研究进展[J].园艺学报,2011,38(2):371-378.JIANG Tianmei,YIN Xueren,WANG Ping,SUN Chongde,XU Changjie,LI Xian,CHEN Kunsong. Research advance in regulation of ethylene during ripening and senescence of non-climacteric fruit[J]. Acta Horticulturae Sinica,2011,38(2):371-378.
• [10] IRELAND H S,GUNASEELAN K,MUDDUMAGE R,TACKEN E J,PUTTERILL J,JOHNSTON J W,SCHAFFER R J. Ethylene regulates apple(Malus′domestica)fruit softening through a dose′time-dependent mechanism and through differential sensitivities and dependencies of cell wall-modifying genes[J]. Plant&Cell Physiology,2014,55(5):1005.
• [11] LIU Y Z,TANG P,TAO N G,XU Q,PENG S A,DENG X X,XIANG K S,HUANG R H. Fruit coloration difference between Fengwan,a late-maturing mutant and its original cultivar Fengjie72-1 of navel orange(Citrus sinensis Osbeck)[J]. Journal of Plant Physiology&Molecular Biology,2006,32(1):31-36.
• [12] ZHANG Y J,WANG X J,WU J X,CHEN S Y,CHEN H,CHAI L J,YI H L. Comparative transcriptome analyses between a spontaneous late-ripening sweet orange mutant and its wild type suggest the functions of ABA,sucrose and JA during citrus fruit ripening[J]. Plos One,2014,9(12):e116056.
• [13] WU J,XU Z,ZHANG Y,CHAI L,YI H,DENG X. An integrative analysis of the transcriptome and proteome of the pulp of a spontaneous late-ripening sweet orange mutant and its wild type improves our understanding of fruit ripening in Citrus[J]. Journal of Experimental Botany,2014,65(6):1651-1671.
• [14]钟八莲,赖晓桦,杨斌华,米兰芳,谢上海,黄彩英,杨文侠,张湟.纽荷尔脐橙芽变早熟品种—赣南早脐橙[J].中国南方果树,2013,42(2):48-51.ZHONG Balian,LAI Xiaohua,YANG Binhua,MI Lanfang,XIE Shanghai,HUANG Caiying,YANG Wenxia,ZHANG Huang.‘Gannanzao’,a new early-ripening navel orange cultivar of‘Newhall’[J]. South China Fruits,2013,42(2):48-51.
• [15]米兰芳.橙汁加工品种综合品质分析与评价[D].武汉:华中农业大学,2009.MI Lanfang. Comprehensive analysis evaluation of quality of orange juice processing cultivars[D]. Wuhan:Huazhong Agricultural University,2009.
• [16] PAN X,WELTI R,WANG X. Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry[J]. Nature Protocols,2010,5(6):986-992.
• [17] LIU Y,LIU Q. Efficient isolation of RNA from fruit peel and pulp of ripening navel orange(Citrus sinensis Osbeck)[J]. Journal of Huazhong Agricultural University,2006,25(3):300-304.
• [18] XU Q,CHEN L L,RUAN X,CHEN D,ZHU A,CHEN C,BERTRAND D,JIAO W B,HAO B H,LYON M P. The draft genome of sweet orange(Citrus sinensis)[J]. Nature Genetics,2013,45(1):59.
• [19]中国国家标准化管理委员会.脐橙:GB/T 21488—2008[S].北京:中国标准出版社,2008.China National Standardization Management Committee. Navel orange:GB/T 21488—2008[S]. Beijing:China Standards Press,2008.
• [20]续丽红,陈健美,谢丽红,钟八莲,米兰芳.赣南早脐橙果实成熟过程中主要品质指标的变化[J].中国南方果树,2016,45(2):65-68.XU Lihong,CHEN Jianmei,XIE Lihong,ZHONG Balian,MI Lanfang. Changes of main quality Indexes of‘Gannanzao’navel orange during fruit ripening[J]. South China Fruits,2016,45(2):65-68.
• [21] ENRIQUETA A,MANUEL C,MARíAJESúS R,LORENZO Z A,MANUEL T. Regulation of color break in citrus fruits.Changes in pigment profiling and gene expression induced by gibberellins and nitrate,two ripening retardants[J]. Journal of Agricultural&Food Chemistry,2006,54(13):4888.
• [22] WANG Y,WANG Y,JI K,DAI S,HU Y,SUN L,LI Q,CHEN P,SUN Y,DUAN C. The role of abscisic acid in regulating cucumber fruit development and ripening and its transcriptional regulation[J]. Plant Physiology&Biochemistry,2013,64(5):70.
• [23] WANG X,YIN W,WU J,CHAI L,YI H. Effects of exogenous abscisic acid on the expression of citrus fruit ripening-related genes and fruit ripening[J]. Scientia Horticulturae,2016,201:175-183.
• [24] MA Y,SZOSTKIEWICZ I,KORTE A,MOES D,YANG Y,CHRISTMANN A,GRILL E. Regulators of PP2C phosphatase activity function as abscisic acid sensors[J]. Science,2009,324(5930):1064.
• [25] FUJII H,SHIMADA T,SUGIYAMA A,NISHIKAWA F,ENDO T,NAKANO M,IKOMA Y,SHIMIZU T,OMURA M. Profiling ethylene-responsive genes in mature mandarin fruit using a citrus 22K oligoarray[J]. Plant Science,2007,173(3):340-348.
• [26] RODRIGO M J,ZACARIAS L. Effect of postharvest ethylene treatment on carotenoid accumulation and the expression of carotenoid biosynthetic genes in the flavedo of orange(Citrus sinensis L. Osbeck)fruit[J]. Postharvest Biology&Technology,2007,43(1):14-22.
• [27] KOMATSU A,TAKANOKURA Y,MORIGUCHI T,OMURA M,AKIHAMA T. Differential expression of three sucrose-phosphate synthase isoforms during sucrose accumulation in citrus fruits(Citrus unshiu Marc.)[J]. Plant Science,1999,140(2):169-178.