陈健美;谢丽红;周娟;江小美;钟八莲;李淑惠;杨斌华;喻芳琴;

• 1:赣南师范大学·国家脐橙工程技术研究中心
• 2:于都县农业与粮食局
• 3:于都县果茶局

摘要(Abstract)：

【目的】明确早熟品种‘赣南早’脐橙(wild type,WT)与其早熟性状回复型突变体(mutant type, MT)在生理和转录水平的差异,探究柑橘果实成熟的调控机制。【方法】测定MT和WT的果实品质及成熟相关生理指标,采用RNASeq分析MT和WT果实的转录差异。【结果】MT具有稳定的早熟性状回复现象。MT和WT果皮的各可溶性糖含量差异显著。MT和WT有机酸含量在果肉间及果皮间差异均显著。MT果皮的赤霉素(gibberellin,GA)、吲哚乙酸(indole acetic acid,IAA)和茉莉酸(jasmonic acid,JA)显著高于WT,脱落酸(abscisic acid,ABA)则相反。转录组测序分析表明,MT与WT果皮间DEGs数量为980个,果肉间为289个。在果皮DEGs中,有38种GO分类、6个KEGG代谢通路被显著性富集,而果肉中未见GO分类和KEGG代谢通路的显著性富集。ABA合成基因CsNCED1在MT果皮和果肉中均下调表达,而分解基因CsCYP707A1在MT果皮中上调表达。【结论】MT成熟期比WT推迟约30 d。MT果皮GA含量及GA合成基因CsCPS1、CsKAO表达量均高于WT,可能与果皮的褪绿延迟相关。MT果皮ABA积累抑制可能受合成基因CsNCED1下调表达及分解基因CsCYC707A1上调表达的影响。MT和WT果皮的生理和转录组水平差异均比果肉间差异大,说明果皮在柑橘果实成熟过程中具有重要作用。

关键词(KeyWords)： ‘赣南早’脐橙;回复型突变;果实成熟;果实品质;转录组

Abstract:

Keywords:

基金项目(Foundation): 江西省科技重大项目(20152ACF60007);; 江西省青年基金项目(20132BAB2014018);; 江西省科技支持项目(20121BBF60023,20132BBF60026);; 2013年中央财政农业科技推广专项资金

作者(Author): 陈健美;谢丽红;周娟;江小美;钟八莲;李淑惠;杨斌华;喻芳琴;

Email:

DOI:

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