宣利利;欧春青;王斐;王志刚;程飞飞;李振茹;姜淑苓;

• 1:中国农业科学院果树研究所
• 2:农业部果树种质资源利用重点开放实验室
• 3:北京市大兴区林业局

摘要(Abstract)：

中矮1号是中国农业科学院果树研究所选育的梨优良矮化砧木,为研究其矮化机理,利用RT-PCR结合RACE方法克隆了梨矮化砧木中矮1号的1个GA20-氧化酶基因,并对该基因及其氨基酸序列以及该基因在不同生长类型梨品种中不同时期新梢叶片中的表达进行了分析。结果表明,该基因全长1 179 bp,推导编码392个氨基酸,其编码的蛋白质具有GA20-氧化酶基因家族所具有的功能活性位点和特征保守单元,该基因与苹果GA20-氧化酶基因氨基酸序列一致性达91.58%,因此确定该基因为中矮1号GA20-氧化酶基因,将其命名为PcGA20ox1(Gen-Bank登录号为HQ833589)。PcGA20ox1基因在中矮1号、亲本锦香和对照早酥3个品种不同发育阶段新梢叶片中的表达强度均为中矮1号<锦香<早酥,与植株高矮表现一致,表明PcGA20ox1基因的表达强弱与植株高矮相关。

关键词(KeyWords)： 梨;矮化砧木;赤霉素;GA20-氧化酶基因;基因表达

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项;; 院区合作大兴区西洋梨产业升级关键技术研究与示范项目;; 农业科技成果转化资金项目(2010GB23260565)

作者(Author): 宣利利;欧春青;王斐;王志刚;程飞飞;李振茹;姜淑苓;

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参考文献(References)：

• [1]JIA Jing-xian.Pear dwarfing and compact planting[M].Beijing:Jin-dun Press,1995.贾敬贤.梨矮化密植栽培[M].北京:金盾出版社,1995.
• [2]JIANG Shu-ling,JIA Jing-xian,JI Bao-sheng,MA Li.A dwarfrootstock of pear Zhongai 1[J].China Fruits,2000(3):1-3.姜淑苓,贾敬贤,纪宝生,马力.梨矮化砧木中矮1号[J].中国果树,2000(3):1-3.
• [3]JIANG Shu-ling,JIA Jing-xian,WANG Fei,OU Chun-qing,WANG Zhi-gang,XUAN Li-li,CHENG Fei-fei,MA Li.Study on the dwarfing effect of three pear dwarfing inter-stocks on graftingtrees[J].Scientia Agricultura Sinica,2010,43(23):4886-4892.姜淑苓,贾敬贤,王斐,欧春青,王志刚,宣利利,程飞飞,马力.三个梨树中间砧木对嫁接树的矮化效应[J].中国农业科学,2010,43(23):4886-4892.
• [4]CHEN Chang-lan.Study on the biological characteristics and dwarf-ing mechanism of dwarf types of pears[D].Shenyang:Shenyang Agri-culture University,1996.陈长兰.梨树矮型的生物学特性及其致矮机理的研究[D].沈阳:沈阳农业大学,1996.
• [5]LEE D J,ZEEVAART J A.Regulation of gibberellin 20-oxidase1expression in spinach by photoperiod[J].Planta,2007,10:425-436.
• [6]RADI A,LANGE T,KOSHIKA M,PIMENTA LANGE M J.Ectopicexpression of pumpkin gibberellin biosynthesis and development oftransgenic Arabidopsis plants[J].Plant Physiology,2006,140:528-536.
• [7]ANDREW P,PETER H.lsolation and expression of three gibberellin20-oxidase cDNA clones from Arabidopsis[J].Plant Physiol,1995,108:1049-1057.
• [8]XIAO Jing-hua.Cloning and functional analysis of GA 20-oxidaseand GA 2-oxidase genes in tomato[D].Wuhan:Central China Agri-cultural University,2005.肖景华.番茄GA20-氧化酶和GA20-氧化酶基因的克隆与功能分析[D].武汉:华中农业大学,2005.
• [9]HYTONEN T,ELOMAA P,MORITZ T,JUNTTILA O.Gibberellinmediates day length-controlled differentiation of vegetative meris-tems in strawberry(Fragaria×ananassa Duch)[J].BMC Plant Biology,2009,9(18):1-12.
• [10]KUSABA S,HONDA C,KANO MURAKAMI Y.Isolation and ex-pression analysis of gibberellin 20-oxidase homologous gene in ap-ple[J].Journal of Experimental Botany,2001,52(355):375-376.
• [11]CHEN Chang-lan,JIA Jing-xian,HOU Xiao,GONG Xin,JIANGShu-ling,LI Sheng-ben.Study on the anatomy and enzyme activityassay of dwarfing inter-stocks on grafting trees[J].Chinese Agricul-tural Science Bulletin,2000,16(2):20-21.陈长兰,贾敬贤,侯潇,龚新,姜淑苓,李盛本.梨树矮化中间砧嫁接树的解剖及酶活性测定[J].中国农学通报,2000,16(2):20-21.
• [12]JIA Jing-xian,CHEN Chang-lan.Study on the growth,anatomy andbiochemistry of some compact-type pear selections[J].Acta Horti-culturae Sinica,1985,12(4):228-231.贾敬贤,陈长兰.紧凑型梨的生长、解剖和生化特性研究[J].园艺学报,1985,12(4):228-231.
• [13]YAMAGUCHI S,SUN T P,KAWAIDE H,KAMIYA Y.The GA2locus of Arabidopsis thaliana encodes ent-kaurene synthase of gib-berellin biosynthesis[J].Plant Physiol,1998,116:1271-1278.
• [14]SANDRA E D,JENNIFE J S,CHRIS A H,ANDREW T P,JAMESB R.The pea gene LH encodes ent-kaurene oxidase[J].Plant Physi-ology,2004,134:1123-1134.
• [15]UEGUCHI-TANAKA M,AAHIKARI M,NAKAJIMA M,ITOH H,KATOH E,KOBAYASHI M,CHOW T Y,HSING Y I,KITANOH,YAMAGUCHI I,MATSOUKA M.Gibberellin insensitive dwarfencodes a soluble receptor for gibberellins[J].Nature,2005,437:693-698.
• [16]YU Hui-fang,CAO Jia-shu,WANG Yong-qin.Hormones regula-tion in plant dwarfing mutants[J].Chinese Bulletin of Life Sciences,2002,14(2):85-88.虞慧芳,曹家树,王永琴.植物矮化突变体的激素调控[J].生命科学,2002,14(2):85-88.
• [17]DAYAN J,SCHWARZKOPF M,AVNI A,ALONI R.Enhancingplant growth and fiber production by silencing GA 2-oxidase[J].Plant Biotechnology Journal,2010,8:425-435.
• [18]MONNA L,KITAZAWA N,YOSHINO R,SUZUKI J,MASUDAH,MAEGARA Y,TANJI M,SATO M,NASU S,MINOBE Y.Po-sitional cloning of rice semi-dwarfing gene,sd-1:rice“Green Revo-lution Gene”encodes a mutant enzyme involved in gibberellin syn-thesis[J].DNA Research,2002,9(1):11-17.
• [19]TAN Xin,YANG Hong,QIAO Ding-jun,MA Xin-rong.Construc-tion of siRNA plant expression vector interfered with GA 20-oxidaseand production of dwarf tobacco[J].Chinese Journal of Applied andEnvironmental Biology,2008,14(1):48-52.谈心,杨宏,乔定君,马欣荣.干扰烟草GA20-氧化酶siRNA植物表达载体的构建及矮化烟草的产生[J].应用与环境生物学报,2008,14(1):48-52.
• [20]WU Jing,KONG Xiu-ying,GAO Li-feng,REN Zheng-long,JIAJi-zeng.Isolation and analysis of TaGA20ox2 genes in wheat[J].Sci-entia Agricultura Sinica,2009,42(10):3405-3412.武晶,孔秀英,高丽峰,任正隆,贾继增.小麦TaGA20ox2基因的克隆及分析[J].中国农业科学,2009,42(10):3405-3412.
• [21]ZHANG Zhi-hua,LIU Xin-cai,LIU Yan-hong,GAO Yi,WANGHong-xia.Study on relationship between hormone and growth vigorof young Juglans regia[J].Scientia Silvae Sinicae,2006,42(9):131-133.张志华,刘新彩,刘彦红,高仪,王红霞.核桃幼树内源激素与生长势的关系[J].林业科学,2006,42(9):131-133.