RAPD标记鉴定果树芽变的可行性分析The Possibility of Using RAPD Marker for the Identification of Fruit Sport
房经贵,章镇,周兰华,陈崇顺,王三红
摘要(Abstract)：
在综合分析果树芽变的遗传特点以及RAPD标记技术的原理与存在问题的基础上，结合已有的研究报道，对RAPD标记应用于果树芽变鉴定中的可行性进行了探讨。对于不同的果树芽变类型，RAPD标记技术表现出不同的鉴定可行性。同时，对某些有关的问题提出了相应的建议。
关键词(KeyWords)： 果树芽变;RAPD标记;鉴定
基金项目(Foundation): 江苏省科技攻关!(BE99360);;国家留学基金资助项目!(G200002)
作者(Author): 房经贵,章镇,周兰华,陈崇顺,王三红
Email:
参考文献(References)：
- 1 沈德绪主编.果树育种学M.北京中国农业出版社,1997.55～64.
- 2 沈德绪,王元裕,陈力耕编著.柑橘遗传育种学M.北京科学出版社,1998.159～192.
- 3 刘春林,官春云,李恂.关于植物随机引物扩增多态性DNA标记的可靠性问题J.植物生理学通讯,2000,36(1):56～59.
- 4 Debener T Janakiram T Mattiesch L. Sports and seedlings of rose varieties analysed with molecular markersJ. Plant Breeding 2000 11971～74 .
- 5 Kaemmer D Afza R Weising K et al. Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana Musa spp. J.Bio/Technogy 1992101 030～1 035.
- 6 王三红,陈力耕,章镇,等.RAPD在柑橘品系鉴别上的应用J. 果树科学,2000,17(1):70～72.
- 7 Nybom H. DNA fingerprints in sports of ‘Red Delicious’ applesJ. Hortscience 1990 251 641～1 642.
- 8 Nybom H. DNA fingerprinting—A useful tool in fruit breedingJ. Euphytica 1994 77 59～64.
- 9 Hong Yuyang Hanna Schmidt. Selection of a mutant from adventitious shoot formed in X ray treated cherry leaves and differentiation of standard and mutant with RAPDsJ. Euphytica 1994 7789～92.
- 10 Ye GN Hemmat MA Lohdi NF et al. Long primers for RAPD mapping and fingerprinting of grape and pearJ. Bio Techniques 1996 20368～371.
- 11 Ye GN Soylemezoglu G Weeden NF. Analysis of the relationship between grapevine cultivarssports and clones via DNA fingerprintJ. Vitis 1998 3733～38.
- 12 Wolf K Peters-van-Rhijn J. Rapid detection of genetic variability in chrysanthemum Dendrathema grandiflora Tzveleo using random primersJ. Heredity 1993 71335～341.
- 13 Weising K Nybom H Wolff K. DNA fingerprinting in plants and fungiM. Boca RatonCRC Press 1995.
- 14 Trigiano RN Scott MC Caetano-Anolles G. Genetic signatures from amplification profiles characterise DNA mutation in somatic and radiation induced sports of chrysanthemumJ. J Amer Soc Hort Sci 1998 123642～646.
- 15 Williams JG Kubelik AR Livak KJ et al. DNA polymorphism amplified by arbitrary primers are useful genetic markersJ. Nucl Acids Res 1990186 531～6 535.
- 16 Welsh J Peter C McClelland M. Polymorphisms generated by arbitrarily primed PCR in the mouse application to strain identification and genetic mappingJ. Nucleic Acid Res 1990 19303～306.
- 17 刘春林.植物RAPD标记的可靠性研究J.生物技术通报,1999, (2):31～34.
- 18 盖树鹏,孟祥栋.利用RAPD技术进行植物性状标记及辅助选择J.生物技术通报,1999,(6):33～38.
- 19 Gustavo CA Peter MG. DNA markers—Protocols Applications and OverviewsM. New York Wiley-Vch Press 1998.
- 20 刘大钧主编.细胞遗传学M.北京:中国农业出版社,1999.
- 21 浙江农业大学主编.遗传学M.北京:农业出版社,1986.
- 22 沈珝琲,方德福. 真核基因表达调控M. 北京:高等教育出版社,1997.
- 23 Margaret GKDamon L.Transposable elements as sources of variation in animals and plantsJ. Proc Natl Acad Sci USA,1997 947 704～7 711.
- 24 Asins MJ Monforte AJ Merstre PF. Citrus and prunus copia-like retrotransposonsJ. Theor Appl Genet 1999 99 503～510.
- 25 Henning W. GenetikM. Springer-Verlag Berlin Heidelberg 1995.
- 26 Pieter V Rene H Marjo B et al. AFLP a new technique for DNA fingerprintingJ. Nucleic Acids Res 1995 2314 407～4 414.
- 27 房经贵,刘大钧,章镇,等.两个芒果品种的AFLP指纹图谱J.南 京农业大学学报,1999,22(2):25～27.
- 28 Richard GHC. Detection of mutations in DNAJ. Current Opinion in Biotechnology 1992 324～30.