朱银飞;郭玉;刘晓琳;阿地力·沙塔尔;马荣;

• 1:新疆农业大学林业研究所
• 2:新疆农业大学林学与园艺学院

摘要(Abstract)：

【目的】探讨对核桃基腐病(Pythium oligandrum Drechsler)有较好抑制效果的菌株Gy-11的最优发酵条件,提高其次生代谢产物的产量。【方法】开展了最适培养基和最优碳氮源的筛选,以菌体生物量和发酵液对病原菌的抑菌活性为指标,采用单因素方法对菌株的发酵条件进行优化。【结果】菌株Gy-11在LB培养基和NB培养基中生长量最大;生长所需的最佳碳源为葡萄糖,最佳氮源为蛋白胨;菌株Gy-11最佳发酵条件为温度25℃,p H 7.0,装液量50 m L/250m L,接种量体积分数为6%,转速为180 r·min~(-1)。【结论】明确了菌株Gy-11的最适碳氮源和最优发酵条件为进一步研发生防制剂提供理论基础。

关键词(KeyWords)： 核桃基腐病菌;枯草芽孢杆菌;发酵条件;筛选优化

Abstract:

Keywords:

基金项目(Foundation): 新疆维吾尔自治区科技厅计划项目(201231110);; 森林培育重点学科项目

作者(Author): 朱银飞;郭玉;刘晓琳;阿地力·沙塔尔;马荣;

Email:

DOI: 10.13925/j.cnki.gsxb.20150053

参考文献(References)：

• [1]XI Rongting,ZHANG Yiping.Chinese walnut[M].Beijing:China Forestry Publishing House,1992.郗荣庭,张毅萍.中国核桃[M].北京:中国林业出版社,1992.
• [2]LI Zhongxin,YANG Liling,ABULIZI·Basiti,YAN Shengkun,LIU Kui,ZHU Zhanjiang,YANG Zhongqiang,LIU Jia,SHEN Xiaohe,Maihemujiang,MA Wenqiang.The Development of walnut mechanization of production equipment in Xinjiang[J].Xinjiang Agricultural Sciences,2014,51(5):973-980.李忠新,杨莉玲,阿布力孜·巴斯提,闫圣坤,刘奎,朱占江,杨忠强,刘佳,沈晓贺,买合木江,马文强.新疆核桃产业化发展研究[J].新疆农业科学,2014,51(5):973-980.
• [3]SHANG Jing,LIU Xuefeng,ADILI·Shataer,PAN Cunde,ZHAO Zhenyu,MA Hongxia.Pathogen identification of Walnut Stalk rot disease[J].Scientia Silvae Sinicae,2010,46(12):97-100+192.商靖,刘雪峰,阿地力·沙塔尔,潘存德,赵震宇,马红霞.核桃基腐病的病原鉴定[J].林业科学,2010,46(12):97-100+192.
• [4]SHANG Jing,WANG Gang,NUREMAN·Kebier,ADILI·Shataer,PAN Cunde.A preliminary report on the survey and control test of surroundings of occurrence of walnut stalk rot[J].Journal of Xinjiang Agricultural University,2010,33(4):329-334.商靖,王刚,努热曼·克比尔,阿地力·沙塔尔,潘存德.核桃基腐病发病环境调查及防治试验初报[J].新疆农业大学学报,2010,33(4):329-334.
• [5]DINUR,BUZAYNAPGUL·Esak,WANG Xiaowei.The kinds and activities of cell wall degrading enazmes produced by Pythium oligandrum Drechsler[J].Forest by Product and Speciality in China,2013(5):28-30.迪丽努尔,布再纳普古丽·依萨克,王晓炜.核桃基腐病菌产生细胞壁降解酶的种类及其活性分析[J].中国林副特产,2013(5):28-30.
• [6]TURHONGJIANG·Aierken,XU Fangyuan,ADILI·Shataer,YAN Jun,AI li.Occurrence law and control of walnut stalk rot disease in moyu country,Xinjiang[J].Journal of Xinjiang Agricultural University,2014,37(5):393-398.吐尔洪江·艾尔肯,徐方媛,阿地力·沙塔尔,闫军,艾力.新疆墨玉县核桃基腐病的发生规律与防治研究[J].新疆农业大学学报,2014,37(5):393-398.
• [7]GAO Fen,LI Jinghong,ZHANG Nasha,WANG Junhong,WANG Mengliang.In vitro screening of antagonistic Bacillus against Alternaria alternata and optimization of fermentation factors[J].Plant Protection,2014,40(3):70-75.高芬,李静虹,张娜莎,王俊宏,王梦亮.‘赞皇枣’黑腐病菌拮抗芽胞杆菌的筛选及发酵条件优化[J].植物保护,2014,40(3):70-75.
• [8]YANG Hua,JI Mingshan,LI Guangxu,ZHANG Guangren.Influence of different fermentable conditions on growth of antagonistic Bacillus sp.for Botryosphaeria berengeriana f.sp.piricola[J].Journal of Fruit Science,2007,24(6):799-802.杨华,纪明山,李广旭,张广仁.不同发酵条件对苹果轮纹病拮抗细菌生长的影响[J].果树学报,2007,24(6):799-802.
• [9]TIAN Chengming,HUANG Huayi,MA Rong,LIANG Yingmei,YOU Chongjuan.An antagonistic strains of poplar anthracnose fungs Colletotrichum gloeosporooides and its application:China,CN103333844A[P].20131002.田呈明,黄华毅,马荣,梁英梅,游崇娟.一株拮抗杨树炭疽病菌的菌株及其应用:中国,CN103333844A[P].20131002.
• [10]CHAI Haiyun.The study on fermentation technology、characterization and cloning of the fibrinolytic enzyme from Bacillus amyloliquefaciens[D].Guangzhou:South China University of Technology,2013.柴海云.解淀粉芽孢杆菌纤溶酶的发酵优化、酶学性质及其基因的表达[D].广州:华南理工大学,2013.
• [11]HUANG Yiying,LIU Runye,CUI Jia,LI Huifen,LONG Zhangfu.Optimization of fermentation conditions and antibacterial substances of antagonistic bacteria Q6 against ginger bacterial[J].Journal of Sichuan University(Natural Science Edition),2008,45(6):1504-1508.黄译影,刘润叶,崔佳,李惠芬,龙章富.姜瘟病拮抗细菌Q6发酵条件的优化及抑菌物质的初步研究[J].四川大学学报(自然科学版),2008,45(6):1504-1508.
• [12]LIN Tianxing,GUAN Qinlan,GONG Mingfu.Optimization of fermentation conditions for endophytic bacteria strains YBS106[J].Science&Technology Information,Science&Technology Information,2014(6):140-141.林天兴,管芩澜,龚明福.内生细菌YBS106菌株发酵条件优化[J].科技资讯,2014(6):140-141.
• [13]CHE Xiaoxi,LI Shezeng,LI Xiaokun.Research on the design of the medium for the amyloliquefaciens bacillus fermentation and optimization of its fermentation condition[J].Journal of Anhui Agricultural Sciences,2010,38(18):9402-9405.车晓曦,李社增,李校堃.1株解淀粉芽孢杆菌发酵培养基的设计及发酵条件的优化[J].安徽农业科学,2010,38(18):9402-9405.
• [14]XIN Shanshan,QI Gaofu,ZHU Fayin,LU Jinzhong,LI Qingqing,WANG Shengying,LIU Yanjun,CHEN Jingyuan,WANG Yixun,ZHAO Xiuyun.Optimization of fermentation condition for Bacillus amyloliquefaciens WH1 and its biological control effect on Colletotrichum gloeosporioides[J].Journal of Huazhong Agricultural University,2011,30(4):411-415.信珊珊,祁高富,朱发银,逯晋忠,李菁菁,王圣英,柳艳军,陈京元,王义勋,赵秀云.1株解淀粉芽孢杆菌发酵条件的优化及其对油茶炭疽病的防效[J].华中农业大学学报,2011,30(4):411-415.
• [15]CHEN Xuewu,HU Bohan,LIU Suchun,REN Zhilu,JIANG Jiala,LI Jialian.Screening of the high lipid production strains and optimizing its fermented conditions in liquid state[J].Journal of Hunan Agricultural University(Natural Sciences),2009,35(4):437-440.陈学武,胡博涵,刘素纯,任智璐,蒋加拉,李佳莲.产油脂菌株的筛选及其液态发酵条件的优化[J].湖南农业大学学报(自然科学版),2009,35(4):437-440.
• [16]YI Youjin,LUO Kun,BO Lianyang,LIU Erming,LUO Kuan.The optimal fermentative condition of endophytic bacteria B001 in tobacco in 5 L fermentation container[J].Journal of Hunan Agrieultural University(Natural Sciences),2007,33(6):747-750.易有金,罗坤,柏连阳,刘二明,罗宽.烟草内生细菌B001菌株5 L发酵罐最佳发酵条件[J].湖南农业大学学报(自然科学版),2007,33(6):747-750.
• [17]HONG Peng,AN Guodong,HU Meiying,GENG Peng,SANG Song.Optimizing fermentation condition for Bacillus amyloliquefaciens HF01[J].Chinese Journal of Biological Control,2013,29(4):569-578.洪鹏,安国栋,胡美英,耿鹏,桑松.解淀粉芽孢杆菌HF01发酵条件优化[J].中国生物防治学报,2013,29(4):569-578.
• [18]ZHU Maoshan,ZHANG Zehua,LYU Zhenhuan.Fermentation condition of antagonistic Actinomycete S091[J].Journal of Shenyang Agricultural University,2014,45(2):209-212.朱茂山,张泽华,吕振环.拮抗放线菌S091菌株的发酵条件[J].沈阳农业大学学报,2014,45(2):209-212.
• [19]GE Pinghua.Screening of marine antagonistic bacteria and its inhibition of Sclerotinia sclerotiorum[D].Nanjing:Nan Jing Agricultural University,2013.葛平华.海洋颉颃菌的筛选及其对核盘菌抑制作用的研究[D].南京:南京农业大学,2013.
• [20]DUFFY B K,OWNLEY B H,WELLER D M.Siol chemical and physicalproperties associated with suppression of taleall of wheat by Trichoderma koningii[J].Phytopahtoloy,1997,87:1118-1124.