核桃基腐病拮抗细菌发酵条件的优化Condition optimization for fermentation of antagonistic strain of walnut stalk rot disease
朱银飞;郭玉;刘晓琳;阿地力·沙塔尔;马荣;
摘要(Abstract):
【目的】探讨对核桃基腐病(Pythium oligandrum Drechsler)有较好抑制效果的菌株Gy-11的最优发酵条件,提高其次生代谢产物的产量。【方法】开展了最适培养基和最优碳氮源的筛选,以菌体生物量和发酵液对病原菌的抑菌活性为指标,采用单因素方法对菌株的发酵条件进行优化。【结果】菌株Gy-11在LB培养基和NB培养基中生长量最大;生长所需的最佳碳源为葡萄糖,最佳氮源为蛋白胨;菌株Gy-11最佳发酵条件为温度25℃,p H 7.0,装液量50 m L/250m L,接种量体积分数为6%,转速为180 r·min~(-1)。【结论】明确了菌株Gy-11的最适碳氮源和最优发酵条件为进一步研发生防制剂提供理论基础。
关键词(KeyWords): 核桃基腐病菌;枯草芽孢杆菌;发酵条件;筛选优化
基金项目(Foundation): 新疆维吾尔自治区科技厅计划项目(201231110);; 森林培育重点学科项目
作者(Author): 朱银飞;郭玉;刘晓琳;阿地力·沙塔尔;马荣;
Email:
DOI: 10.13925/j.cnki.gsxb.20150053
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