崔镁沙;庄艳;申飞;杨丽丽;王忆;吴婷;赵永波;李春敏;韩月澎;陈东玫;杨凤秋;张新忠;韩振海;

• 1:中国农业大学园艺植物研究所
• 2:河北省农林科学研究院昌黎果树研究所
• 3:中国科学院武汉植物园

摘要(Abstract)：

【目的】苹果果实轮纹病是我国苹果的重要病害之一,研究果实相关抗病性基因对苹果育种有重要意义。【方法】以苹果(Malus domestica)‘红玉’ב金冠’杂交实生树为材料,对果实接种5个轮纹病菌(Botryosphaeria dothidea)后调查其潜伏期、病斑大小、发病率、病斑扩展速率等指标,运用已有的SSR图谱和苹果基因组注释信息,对苹果果实轮纹病进行QTL(Quantitative Trait Loci)分析并对苹果轮纹病抗病基因进行预测。【结果】得到与苹果果实轮纹病抗病性相关的QTLs共29个,分布在LG5、LG10、LG12、LG15、LG17等5个连锁群上。在QTLs位点的23个侧翼SSR标记中,有18个可以从苹果基因组上相对应的contig位置截取到6个区间的序列信息共35 Mb,共含5 620个基因。最终根据苹果基因注释预测得到与抗病相关的基因107个,其中与抗病蛋白相关的基因60个,编码亮氨酸结构域的基因3个;编码防御酶的基因41个;编码与病程相关蛋白有关的基因3个。【结论】预测到与苹果轮纹病相关的基因107个,为进一步进行抗病性基因挖掘提供数据。

关键词(KeyWords)： 苹果;苹果轮纹病;QTL;SSR;抗病基因

Abstract:

Keywords:

基金项目(Foundation): 国家863课题(2011AA001204);; 农业部园艺作物营养与生理重点实验室;; 北京市果树逆境生理与分子生物学重点实验室

作者(Author): 崔镁沙;庄艳;申飞;杨丽丽;王忆;吴婷;赵永波;李春敏;韩月澎;陈东玫;杨凤秋;张新忠;韩振海;

Email:

DOI: 10.13925/j.cnki.gsxb.20140145

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