不同来源猕猴桃雄株花粉特性遗传差异及聚类分析Genetic differences and cluster analysis of pollens from different male kiwifruit germplasm resources
齐秀娟;徐善坤;钟云鹏;陈锦永;林苗苗;孙雷明;方金豹;
摘要(Abstract):
【目的】探究不同来源猕猴桃雄株花粉特性遗传差异。【方法】以美味猕猴桃(Actinidia diliciosa)杂交F1代、中华猕猴桃(A.chinensis)‘琼浆’种子航天搭载实生后代等不同来源的100份猕猴桃雄性单株为研究对象,探讨其花粉粒大小、花粉量及其萌发特性等遗传差异。【结果】美味猕猴桃花药数变异系数大于中华猕猴桃,单药花粉量变异系数小于中华猕猴桃;美味猕猴桃种内同一杂交组合的F1代,除了单花花粉量以外,其他几个性状变异系数都大于不同来源的美味猕猴桃单株;中华猕猴桃种内同一个品种‘琼浆’种子航天搭载的实生后代除了花粉粒直径以外,其他几个性状变异系数均小于不同来源的单株。对上述各项因子分别进行聚类分析,美味猕猴桃花药数明显多于中华猕猴桃和软枣猕猴桃;单花花粉量、萌发率、花粉管长度在中等以上分类中美味猕猴桃占绝对数量;筛选出7株花粉萌发率高且花粉管生长速度快的优株,暗培养3 h平均花粉萌发率73.93%,平均花粉管长度408.49μm。【结论】明确了不同来源猕猴桃雄性单株花粉特性的遗传差异,可以为雄性品种选育及开发利用提供科学依据。
关键词(KeyWords): 猕猴桃;花粉量;萌发率;花粉管长度;遗传差异;聚类分析
基金项目(Foundation): 中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2016-ZFRI);; 河南省现代农业产业技术体系(S2014-11)
作者(Author): 齐秀娟;徐善坤;钟云鹏;陈锦永;林苗苗;孙雷明;方金豹;
Email:
DOI: 10.13925/j.cnki.gsxb.20160039
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