李洪雯;刘建军;陈克玲;关斌;王建辉;何建;何礼;

• 1:四川省农业科学院园艺研究所
• 2:四川省农业科学院

摘要(Abstract)：

【目的】探明欧洲甜樱桃砧木‘SL64’离体叶片再生技术体系。【方法】以‘SL64’的组培苗叶片为试材,从基本培养基、激素配比、暗培养和叶片生理状态等方面对影响离体叶片再生的关键因素进行了研究,建立了SL64砧木离体叶片的高效再生体系。【结果】‘SL64’采用改良DKW培养基为基本培养基再生效果最好,显著优于MS、WPM和QL培养基;最佳激素组合为6-BA 2.5 mg·L-1+IBA 0.05 mg·L-1;接种后5℃下暗培养5 d、然后23℃下暗培养10 d可明显提高愈伤组织发生率和不定芽再生率。【结论】通过以上条件的优化,建立了‘SL64’的高效离体再生技术体系,离体叶片不定芽再生率为90%左右,平均每叶再生不定芽数达5~6芽。

关键词(KeyWords)： 欧洲甜樱桃;‘SL64’;砧木;离体叶片;再生体系

Abstract:

Keywords:

基金项目(Foundation): 四川省财政基因工程专项(2011JYGC05-018-06)

作者(Author): 李洪雯;刘建军;陈克玲;关斌;王建辉;何建;何礼;

Email:

DOI:

参考文献(References)：

• [1]WERTHEIM S J.Cherry rootstocks[M].Rootstock Guide,1998:95-96.
• [2]LI Hong-wen,LIU Jian-jun,CHEN Ke-ling,LI Xu-feng,YANG Yi.Studies on in vitro rapid propagation of the sweet cherry rootstockof CAB series[J].Southwest China Journal of Agricultural Sciences,2007(3):481-485.李洪雯,刘建军,陈克玲,李旭锋,杨毅.CAB系列甜樱桃砧木离体快繁技术研究[J].西南农业学报,2007(3):481-485.
• [3]MURASHIGE T,SKOOG F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Physiol Plant,1962,15:473-497.
• [4]LLOYD G,MCCOWN B.Commercially feasible micropropagation of mountain laurel,Kalmia latifolia,by use of shoot-tip culture[J].Int Plant Prop Soc Proc,1980,30:421-427.
• [5]QUOIRIN M,LEPOIVRE P.Improved media for in vitro culture of Pranus spp.[J].Acta Horti,1977,78(6):437-442.
• [6]TEWARY P K,OKA S.Simplified clonal multiplication of mulberry using liquid shake culture[J].Plant cell,Tissue and Organ Culture,1999,3(4):223-226.
• [7]DUCHEFA BIOCHEMIE B V.Biochemicals plant cell and tissue culture[M].Catalogue,2003-2005:136.
• [8]GAO Xi-ye,LI Ming,LI Xiao-yan,ZHAO Gai-rong,HAN Lixing,HUANG Zhen-guang,QI Xiu-juan,LI Yu-hong.Optimization of a high efficient regeneration system from leaves in vitro in cherry rootstock CAB-6p[J].Journal of Fruit Science,2008,25(4):589-592.高喜叶,李明,李晓燕,赵改荣,韩礼星,黄贞光,齐秀娟,李玉红.樱桃砧木CAB-6p离体叶片再生体系的优化[J].果树学报,2008,25(4):589-592.
• [9]HUANG Wen-jiang,LIU Qing-zhong,FAN Sheng-hua,ZHAO Hong-jun,MA Feng-wang.Studies on system of plant regeneration from leaf explant of cherry rootstocks[J].Acta Horticulturae Sinica,2004,31(2):221-223.黄文江,刘庆忠,樊圣华,赵红军,马锋旺.甜樱桃砧木吉塞拉(Gisela)叶片再生体系研究[J].园艺学报,2004,31(2):221-223.
• [10]WEI Hai-rong,ZONG Xiao-juan,WANG Jia-wei,LIU Qingzhong.Studies on highly frequent and efficient plant regeneration system of sweet cherry tetraploid rootstocks Y1[J].Journal of Fruit Science,2012,29(1):49-52.魏海蓉,宗晓娟,王甲威,刘庆忠.甜樱桃四倍体杂种砧木Y1高频、高效离体再生体系研究[J].果树学报,2012,29(1):49-52.