王西成;宋长年;张彦苹;冷翔鹏;孙欣;房经贵;

• 1:南京农业大学园艺学院

摘要(Abstract)：

【目的】为鉴定葡萄果实赤霉素诱导响应基因,【方法】应用cDNA-RAPD技术,以鲜食葡萄品种‘藤稔’为研究对象,对赤霉素处理后果实发育过程中基因表达进行了mRNA指纹分析。【结果】通过16条随机引物的筛选,共分离得到61个差异表达的转录衍生片段(TDF),其中增强表达或赤霉素诱导下特异性表达TDF42条,抑制表达19条。对其中18个TDF进行了克隆、测序和序列分析发现,17个TDF与NCBI已有序列同源,其中10个为已知功能基因,另有1个TDF无同源序列。对选取的6个TDF进行半定量RT-PCR验证,结果表明3个基因片段在处理条件下均特异表达或上调表达,另外3个基因片段在处理条件下均未见表达或下调表达,这与cDNA-RAPD表达谱结果相一致。【结论】利用cDNA-RAPD技术成功分离了部分受赤霉素诱导的差异表达基因。

关键词(KeyWords)： 葡萄;‘藤稔’;赤霉素;cDNA-RAPD;差异表达基因

Abstract:

Keywords:

基金项目(Foundation): 江苏省高校优势学科项目;; 教育部新世纪优秀人才支持计划项目(NCET-08-0796)

作者(Authors): 王西成;宋长年;张彦苹;冷翔鹏;孙欣;房经贵;

DOI: 10.13925/j.cnki.gsxb.2012.05.016

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