彭宇鸿;傅敏;胡红菊;黄新忠;洪霓;王国平;

• 1:华中农业大学植物科学技术学院·湖北省作物病害监测与安全控制重点实验室
• 2:湖北省农业科学院果树茶叶研究所
• 3:福建省农业科学院果树研究所

摘要(Abstract)：

【目的】明确我国南方梨产区造成早期落叶的果生炭疽菌(Colletotrichum fructicola)的致病力分化状况并建立其室内快速测定方法。【方法】以梨的枝条、叶片和果实为材料,采用不同方法造成伤口后接种果生炭疽菌的强致病力菌株PAFQ32,通过比较各处理的测定效果筛选其室内快速测定方法,并对供试菌株的致病力进行观测和致病类型划分,分析不同菌株致病力分化与其地理来源之间的相关性。【结果】采用梨枝条、叶片和果实对果生炭疽菌致病力的观测结果显示,梨叶片经针刺后接种菌丝块的测定效果明显优于其他处理。供试菌株致病力的测定结果表明,来源于我国南方梨产区的111个果生炭疽菌菌株其致病力可划分为强、中、弱3个类型,其中强致病力菌株17个(15.3%);中等致病力菌株89个(80.2%);弱致病力菌株5个(4.5%)。不同地理来源的果生炭疽菌菌株,其致病类型的分布比例有异。【结论】果生炭疽菌的菌丝块针刺接种梨叶片的方法,可用于其致病力的室内快速测定。来源于我国南方梨产区导致早期落叶的果生炭疽菌存在明显的致病力分化,以中等致病力菌株为优势群体。

关键词(KeyWords)： 梨;果生炭疽菌;菌株;室内测定方法;致病力分化

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发项目(2018YFD0201406）;; 国家梨产业技术体系（CARS-28-16）

作者(Author): 彭宇鸿;傅敏;胡红菊;黄新忠;洪霓;王国平;

Email:

DOI: 10.13925/j.cnki.gsxb.20200030

参考文献(References)：

• [1]刘坤.‘汉水砂梨’早期落叶的诱因调查及初步分析[J].中国果树，2016(2):91-93.LIU Kun. Investigation and preliminary analysis of the causes of early leaves in‘Han Shui Sandy Pear’[J]. China Fruits, 2016(2):91-93.
• [2]柯冠武，郭林榕.南方早熟梨早期落叶、二次开花和果实偏小现象原因及对策[J].中国南方果树，2007,36(1):77-79.KE Guanwu, GUO Linrong. Causes and countermeasures of early defoliation, secondary flowering and fruit smallness in earlymaturing pears in southern China[J]. South China Fruits, 2007,36(1):77-79.
• [3]张桂池.福建梨“二次花”发生情况及其原因研究[D].福州：福建农林大学，2016.ZHANG Guichi. Study on the occurrence and causes of the“reflorescnce”of pear in Fujian province[D]. Fuzhou:Fujian Agricultural and Forestry University, 2016.
• [4]黄新忠，陈义挺，雷龑，蔡盛华，陈小明.福建梨早期大量落叶诱因与防控策略[J].中国农学通报，2010,26(2):91-95.HUANG Xinzhong, CHEN Yiting, LEI Yan, CAI Shenghua,CHEN Xiaoming. Causes and control strategies of a large number of early falling leaves of pear in Fujian[J]. Chinese Agricultural Science Bulletin, 2010, 26(2):91-95.
• [5]陈义挺，刘鑫铭，陈小明，陈婷，王卫，张长和，包建平，黄新忠.福建省梨异常早期落叶初步调查与分析[J].龙岩学院学报，2011,29(5):43-45.CHEN Yiting, LIU Xinming, CHEN Xiaoming, CHEN Ting,WANG Wei, ZHANG Changhe, BAO Jianping, HUANG Xinzhong. Reliminary investigation and analysis of early falling leaves in pear abnormal in Fujian province[J]. Journal of Longyan University, 2011, 29(5):43-45.
• [6] ZHANG P F, ZHAI L F, ZHANG X K, HUANG X Z, HONG N,XU W X, WANG G P. Characterization of Colletotrichum ffructicola, a new causal agent of leaf black spot disease of sandy pear(Pyrus pyrifolia)[J]. European Journal of Plant Pathology,2015, 143(4):651-662.
• [7] FU M, CROUS P W, BAI Q, ZHANG P F, XIANG J, GUO Y S,ZHAO F F, YANG M M, HONG N, XU W X, WANG G P. Colletotrichum species associated with anthracnose of Pyrus spp. in China[J]. Persoonia, 2019, 42(1):1-35.
• [8] PENG L J, YANG Y L, HYDE K D, BAHKALI A H, LIU Z Y.Colletotrichum species on citrus leaves in Guizhou and Yunnan provinces, China[J]. Cryptogamie Mycoscience, 2012, 33(3):267-283.
• [9] WEIR B S, JOHNSTON P R, DAMM U. The Colletotrichum gloeosporioides species complex[J]. Studies in Mycology, 2012,73(1):115-180.
• [10] MANAMGODA D S, UDAYANGA D, CAI L, CHUKEATIROTE E, HYDE K D. Endophytic Colletotrichum from tropical grasses with a new species C. endophytica[J]. Fungal Diversity, 2013, 61(1):107-115.
• [11] VIEIRA W A S, MICHEREFF S J, DE M J M A, HYDE K D,C?MARA M P S. Endophytic species of Colletotrichum associated with mango in northeastern Brazil[J]. Fungal Diversity,2014, 67(1):181-202.
• [12] VELHO A C, ALANIZ S, CASANOVA L, MONDINO P,STADNIK M J. New insights into the characterization of Colletotrichum species associated with apple diseases in southern Brazil and Uruguay[J]. Fungal Biology, 2015, 119(4):229-244.
• [13] WANG Y C, HAO X Y, WANG L, BIN X, WANG X C, YANG Y J. Diverse Colletotrichum species cause anthracnose of tea plants(Camellia sinensis(L.)O. Kuntze)in China[J]. Scientific Reports, 2016, 6:35287.
• [14] PRIHASTUTI H, CAL L, CHEN H, MCKENZIE E H C,HYDE K D. Characterization of Colletotrichum species associated with coffee berries in northern Thailand[J]. Fungal Diversity,2009, 39:89-109.
• [15] LIAO C Y, CHEN M Y, CHEN Y K, WANG T C, SHEU Z M,KUO K C, CHANG P F L, CHUNG K R, Lee M H. Characterization of three Colletotrichum acutatum isolates from Capsicum spp.[J]. European Journal of Plant Pathology, 2012, 133(3):599-608.
• [16] LIMA N B, LIMA W G, TOVAR-PEDRAZA J M, MICHEREFF S J, C?MARA M P S. Comparative epidemiology of Colletotrichum species from mango in northeastern Brazil[J].European Journal of Plant Pathology, 2015, 141(4):679-688.
• [17]王薇.苹果炭疽叶枯病病原学及苹果炭疽病侵染来源研究[D].杨凌：西北农林科技大学，2017.WANG W. Etiology of apple Glomeralla leaf spot caused by Colletotrichum and infection sources of apple bitter rot[D]. Yangling:Northwest Agricultural and Forestry University, 2017.
• [18]李河，周国英，徐建平，朱丹雪.一种油茶新炭疽病原的多基因系统发育分析鉴定[J].植物保护学报，2014,41(5):602-607.LI He, ZHOU Guoying, XU Jianping, ZHU Danxue. Pathogen identification of a new anthracnose of Camellia oleifera in China based on multiple-gene phylogeny[J]. Acta Phytophylacica Sinaca, 2014, 41(5):602-607.
• [19] DIAO Y Z, ZHANG C, LIU F, WANG W Z, LIU L, CAI L, LIU X L. Colletotrichum species causing anthracnose disease of chili in China[J]. Persoonia, 2017, 38:20-37.
• [20] PERES N A, TIMMER L W, ADASKAVEG J E, CORRELL J C. Life styles of Colletotrichum acutatum[J]. Plant Disease,2005, 89(8):784-796.
• [21] ALKAN N, FRIEDLANDER G, MENT D, PRUSKY D,FLUHR R. Simultaneous transcriptome analysis of Colletotrichum gloeosporioides and tomato fruit pathosystem reveals novel fungal pathogenicity and fruit defense strategies[J]. New Phytologist, 2015, 205(2):801-815.
• [22] DE SILVA D D, CROUS P W, ADES P K, HYDE K D, TAYLOR P W J. Life styles of Colletotrichum species and implications for plant biosecurity[J]. Fungal Biology Reviews, 2017, 31(3):155-168.
• [23] JIANG J J, ZHAI H Y, LI H N, WANG Z H, CHEN Y S,HONG N, WANG G P, CHOFONG G N, XU W X. Identification and characterization of Colletotrichum fructicola causing black spots on young fruits related to bitter rot of pear(Pyrus bretschneideriRehd.)inChina[J].CropProtection,2014,58:41-48.
• [24]韦洁玲，黄丽丽，郜佐鹏，柯希望，康振生.苹果树腐烂病室内快速评价方法的研究[J].植物病理学报，2010,40(1):14-20.WEI Jieling, HUANG Lili, GAO Zuopeng, KE Xiwang, KANG Zhensheng. Laboratory evaluation methods of apple Valsa canker disease caused by Valsa ceratosperma sensu Kobayashi[J].Acta Phytopathologica Sinica, 2010, 40(1):14-20.
• [25]孙洁莹，陈出新，谷超，殷豪，齐开杰，张绍铃，吴俊.梨品种资源抗炭疽病鉴定与筛选[J].果树学报，2016,33(增刊):184-195.SUN Jieying, CHEN Chuxin, GU Chao, YIN Hao, QI Kaijie,ZHANG Shaoling, WU Jun. The anthracnose-resistance identification and screening of pear cultivar resources[J]. Journal of Science Fruit, 2016, 33(Suppl.):184-195.
• [26]张蕊，杨石有，刘晓妹，蒲金基，张贺.芒果炭疽菌致病力室内快速测定方法研究[J].黑龙江农业科学，2016(8):51-53.ZHANG Rui, YANG Shiyou, LIU Xiaomei, PU Jinji, ZHANG He. A rapid laboratory determining methods of the pathogenicity of mango anthracnose pathogen[J]. Heilongjiang Agricultural Sciences, 2016(8):51-54.
• [27]刘威，叶乃兴，刘伟，金珊，连玲丽，赖建东，谢运海.茶树炭疽菌的鉴定及致病力分析[J].福建农大学学报(自然科学版)，2015,44(6):581-586.LIU Wei, YE Naixing, LIU Wei, JIN Shan, LIAN Lingli, LAI Jiandong, XIE Yunhai. Identification and pathogenic analysis of the pathogen causing tea plant(Camellia sinensis)anthracnose[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2015, 44(6):581-586.
• [28]李杨，李河，周国英，蒋越西，刘君昂.海南省3种油茶炭疽病菌的致病力及其生物学特性研究[J].热带作物学报，2016,37(10):1956-1961.LI Yang, LI He, ZHOU Guoying, JIANG Yuexi, LIU Jun’ang.Virulence and biological characteristics of three pathogens causing oil tea anthracnose in Hainan province[J]. Chinese Journal of Tropical Crops, 2016, 37(10):1956-1961.
• [29]李江华，杨石有，张蕊，张贺，蒲金基.杧果炭疽菌株对台农1号的致病力分化研究[J].中国南方果树，2017,46(2):77-80.LI Jianghua, YANG Shiyong, ZHANG Rui, ZHANG He, PU Jinji. Study on pathogenicity differentiation of mango Tainong 1anthracnose strains[J]. South China Fruits, 2017, 46(2):77-80.
• [30] BESSHO H, KOMORI S, SOEJIMA J. Simple excised twig assay of Malus species for determining resistance to Valsa canker[J]. Acta Horticulturae, 2004, 658(2):391-397.
• [31]臧睿，黄丽丽，康振生，王旭丽.陕西苹果树腐烂病菌(Cytospora spp.)不同分离株的生物学特性与致病性研究[J].植物病理学报，2007,37(4):343-351.ZANG Rui, HUANG Lili, KANG Zhensheng, WANG Xuli. Biological characteristics and pathogenicity of different isolates of Cytospora spp. isolated from apple trees in Shaanxi province[J].Acta Phytopathologica Sinica, 2007, 37(4):343-351.
• [32] ABE K, KOTODA N, KATP H, SOEJIMA J. Resistance sources to Valsa canker(Valsa ceratosperma)in a germplasm collection of diverse Malus species[J]. Plant Breeding, 2007, 126(4):449-453.
• [33]方敦煌，马永凯，孔光辉，王革，李天飞.云南烟草赤星病菌致病力分化研究[J].西南农业大学学报，2000(1):42-44.FANG Dunhuang, MA Yongkai, KONG Guanghui, WANG Ge,LI Tianfei. An investigation differentiation of pathogenicity in tobacco brown spot(Alternaria alternata)in Yunnan[J]. Journal of Southwest Agricultural University, 2000(1):42-44.
• [34]吴桥，赵秀香，吴元华.辽、冀、鲁黄瓜褐斑病菌（Corynespora cassiicola）培养性状及致病力分化研究[J].沈阳农业大学学报，2017,48(2):207-212.WU Qiao, ZHAO Xiuxiang, WU Yuanhua. Culture traits and pathogenicity differentiation for Corynespora cassiicola, causal agent of cucumber in Liaoning, Hebei and Shandong Provinces[J]. Journal of Shenyang Agricultural University, 2017, 48(2):207-212.
• [35]张优，魏松红，王海宁，李帅，李思博，罗文芳.东北地区水稻纹枯病菌遗传多样性和致病性分析[J].沈阳农业大学学报，2017,48(1):9-14.ZHANG You, WEI Songhong, WANG Haining, LI Shuai, LI Sibo, LUO Wenfang. Genetic diversity and pathogenicity analysis of Rhizoctonia solani isolates causing sneath blight disease of rice in Northeast[J]. Journal of Shenyang Agricultural University, 2017, 48(1):9-14.
• [36]丛子文，钟步飞，黄东益，谢俊，吴文嫱，许云，夏薇，张荣萍，黄小龙.南方淮山炭疽病菌的致病力分化[J].南方农业学报，2018,49(3):501-507.CONG Ziwen, ZHONG Bufei, HUANG Dongyi, XIE Jun, WU Wenqiang, XU Yun, XIA Wei, ZHANG Rongping, HUANG Xiaolong. Pathogenicity differentiation of Dioscorea opposita Thunb. anthracnose pathogen in southern China[J]. Journal of Southern Agriculture, 2018, 49(3):501-507.
• [37]张美鑫，翟立峰，周玉霞，陈晓忍，贾娜娜，洪霓，王国平.我国梨树腐烂病菌致病力分化分析[J].果树学报，2013,30(4):657-664.ZHANG Meixin, ZHAI Lifeng, ZHOU Yuxia, CHEN Xiaoren,JIA Nana, HONG Ni, WANG Guoping. Pathogenicity differentiation of Valsa mali var. pyri causing pear stem canker in China[J]. Journal of Fruit Science, 2013, 30(4):657-664.