彭磊;吴艳;刘小翠;杨鵾;范付华;文晓鹏;

• 1:贵州大学农业生物工程研究院/生命科学学院·山地植物资源保护与种质创新教育部重点实验室
• 2:贵州省安顺市农业科学院
• 3:贵州大学林学院

摘要(Abstract)：

【目的】克隆Ty3-gypsy类反转录转座子反转录酶(RT)序列并分析其特性,为火龙果遗传变异和进化研究提供新的信息。【方法】根据Ty3-gypsy类反转录转座子RT保守区设计简并引物,从红肉和白肉火龙果及其突变体中扩增出430 bp左右的目标片段,回收、克隆、测序获得RT序列,并进行生物信息学分析;采用RT-PCR检测其转录活性。【结果】共获得82条RT序列,其中有55条序列发生终止密码子突变,5条序列发生移码突变;经RT序列对比,火龙果与水稻、拟南芥有较高的同源性;3条RT序列具有转录活性。【结论】火龙果Ty3-gypsy类反转录转座子RT序列具有高度异质性,部分序列具有转录活性,与其他物种存在反转录转座子的横向传递。

关键词(KeyWords)： 火龙果;Ty3-gypsy类反转录转座子;反转录酶;异质性;转录活性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31560549,31260464)

作者(Author): 彭磊;吴艳;刘小翠;杨鵾;范付华;文晓鹏;

Email:

DOI: 10.13925/j.cnki.gsxb.20160286

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