陈延惠,谭彬,李洪涛,胡青霞,叶霞,孟海军,冯建灿

• 1:河南农业大学园艺学院
• 2:河南省果树瓜类生物学重点实验室
• 3:郑州市城市园林科学研究所

摘要(Abstract)：

【目的】为了利用‘突尼斯软籽’石榴开展遗传转化研究,【方法】分别以叶片和茎段为外植体,探讨了不同灭菌时间和不同植物生长调节物质对2种外植体再生的影响,并对2种愈伤组织再生体系进行了比较。【结果】结果表明,茎段比叶片更容易灭菌,但叶片更容易长出绿色致密愈伤组织并分化更多的不定芽,而茎段分化的愈伤相对较为疏松,分化的不定芽也相对较少;来自茎段愈伤组织的不定芽比来自叶片的更容易长高;叶片比茎段再生体系建立所需时间要短;少量翠绿的叶片刀口处可以直接产生不定芽,可以直接利用叶片进行遗传转化的侵染,简化愈伤形成这一步骤,而茎段未发现此现象。在遗传转化试验中,茎段和叶片2种外植体愈伤组织抗生素敏感性试验结果均无显著性差异。【结论】研究结果认为,2者相比,叶片愈伤组织再生体系更适宜于石榴的遗传转化研究。

关键词(KeyWords)： ‘突尼斯软籽’石榴;再生体系;遗传转化

Abstract:

Keywords:

基金项目(Foundation): 河南省科技攻关项目(072102150001);; 郑州市科技攻关项目(083SGYG24123-6)

作者(Author): 陈延惠,谭彬,李洪涛,胡青霞,叶霞,孟海军,冯建灿

DOI: 10.13925/j.cnki.gsxb.2012.04.021

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